Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,422,6300TG56.2% 3.4 / 14.9 17K153T (AAG→ACG) CJ019_01343hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/7);  new base G (9/0);  total (10/7)
Fisher's exact test for biased strand distribution p-value = 8.74e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCC  >  ACB122/1422504‑1422702
                                                                                                                              |                                                                        
cGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTTCGCGTATGCGCAACCCCCGTGGTtgtg                                                                  >  6:42882/1‑135 (MQ=255)
cGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCGTGGTtgtg                                                                  >  6:109346/1‑135 (MQ=255)
      cAACCCTGACAACCACATGGCGCTGTAATCGGCCATTTTTGTTAAACAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGAAGGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCa                                                           <  7:110582/136‑1 (MQ=25)
               gaACGACATGGCGCTGTAAGCGGCCATTGTTGGGAACGAAAGCCACGGGGGAGACCGCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGa                                                  <  8:160103/136‑1 (MQ=255)
                 aCGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGGTGGGCGTATGCGCCAACCCCCTGGGTGTGGGGGCCCAGTGGGGGACt                                                >  2:22936/1‑136 (MQ=25)
                     cATGGCGCTGTAATCGGCCTTTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                             <  7:111898/135‑1 (MQ=255)
                      aTGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTTAGCGCCCCCCGCGTGGTTGTGGGGGGCACGTGGGTGACTTGGAg                                           >  8:242187/1‑136 (MQ=21)
                           gcTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGGGGATGCGCCACCCGCGTTGTTGTGGTGGCCCAGTGGGGGCCTTGGAGGTgt                                       >  8:139300/1‑134 (MQ=21)
                             tGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCCCACCGCGTGGGTGTGGTGGCCAAGTGGGTGCCTTGGTGGTGaaa                                     >  6:48817/1‑135 (MQ=21)
                             tGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCGTGGTTTTGGTGGCCAAGTGGGTAACTTTGAGGTGAAAg                                    >  2:53237/1‑136 (MQ=255)
                                aaTCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTTCGCGGATGCGCCACCCGCGTGGTTGTGGTGGGCCACGGGGTGACTTGGAGGGGTAAGTc                                  >  6:88347/1‑135 (MQ=21)
                                  tCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTTCGCGTTTGCGGCACCCGCGTGGGTGTGGTGGCCCACTGGGTGTCGTGGTGGTGTAAGtgcga                               >  3:79209/1‑132 (MQ=21)
                                  tCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGGTTGCGCAACCCGCGTGGGTGTGGTGGCCAAGTGGGTGACTTTGTGGTGAAATTActc                               >  8:111898/1‑136 (MQ=21)
                                                 aaagaatgCCTCGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACGCGCTTGGATGTGGGGGCCAAGTGGGGGACTTTGAGGTGAAAGTCATCTACACACCCGGCAAg                <  3:46255/136‑1 (MQ=17)
                                                 aaagaaagCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCAGGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAg                <  4:50757/136‑1 (MQ=255)
                                                             gggggAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTa     <  5:109346/135‑1 (MQ=255)
                                                               gggAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGcc  <  1:12890/136‑1 (MQ=40)
                                                                                                                              |                                                                        
CGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCC  >  ACB122/1422504‑1422702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑