Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,725,6390TG46.5% 5.7 / 13.1 43V640G (GTG→GGG) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (7/16);  new base G (4/16);  total (11/32)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAA  >  ACB122/2725511‑2725769
                                                                                                                                |                                                                                                                                  
ggTGCACTACGGGCGCTTCCTGCGGCCCACCCCCCGCGGCCCCGACGCGGGCAGCAAGACGCGCTATGCGGGCGGGGGGCCGGGGGCCGCCGGTGCTGAAGAAATGGTTTACACGGGCGGGGAGGCGGGGCGCAc                                                                                                                              <  8:95952/135‑1 (MQ=255)
ggTGCACTACGGGCGCTGCCTGATCCCCCCCATCCGCGCCCCCGCCTCGGGCAGCAAGCAGCGGTAGGCCGGCCTGGTGCAGCGGGCGGTCGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGCCGGTGCGCAc                                                                                                                              <  4:88489/135‑1 (MQ=255)
ggTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGGGCTGAAGAAATGGTTTACAAGGGCCTGGAGTGGGGgcgcgc                                                                                                                              >  7:485776/1‑133 (MQ=255)
   gCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGGGCCCCgc                                                                                                                           >  3:429530/1‑134 (MQ=255)
               cTTCCTGATGCCCACCAACCGCGGCACCGACCATGGCAGCAAGAAGCGCAATGCCGGCCTGGTGCAGCGGGCCGACGGTGCAGAAGAAATGGATTACAAGGGCCTGGAGTAGGTTCGCCCCGAAAGGTCACCCCt                                                                                                               >  4:53825/1‑135 (MQ=255)
                   ctcacgCCCACCATCCGCGGCACCGCGGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGGGCAGCGGGCCGACGGTGCTTAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGc                                                                                                            <  3:327462/129‑1 (MQ=255)
                   cTGACGCCCACCACCCGCGGCACCGCCGCGGGCATAAAGAAGCGCTTGGCCGGCCGGGTGCCGCGGGCCGACGGTGCTGAAGAAATGGTTAACACGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGc                                                                                                            <  8:76196/134‑1 (MQ=255)
                       tGCCCCCCATCCGCGCCCCCGCCGCGGGCACCAACACGCGCTATGCCGGCCTGGTGCCGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGCGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGc                                                                                                       <  2:301004/135‑1 (MQ=255)
                        gCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCa                                                                                                      <  8:103930/135‑1 (MQ=255)
                         cccACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGGGCGCCGACGGGGCACCGCGGGCCCGGccg                                                                                                     >  3:163919/1‑133 (MQ=255)
                               aTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCCCTGGCCCGGCACTTCcac                                                                                               >  6:279018/1‑134 (MQ=255)
                                tCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcagc                                                                                              <  2:365538/135‑1 (MQ=255)
                                gccGCGGCACCGACGCGGGCAGCAACACGCGCTAGGCCGGCCGGGGGCCGCGGCCCGACGGTGCTGAAGAACTGGTTTACAAGGGCCTGGAGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcagc                                                                                              <  8:76288/134‑1 (MQ=255)
                                cccGGGGCACCGCGGAGGGCAGCAAGAAGGGCTTGGGGGGCCTGGGGCTGGGGGCCGACGGTGATGAAGAAGTGGTTTACATGGGCCTGGAGTCGGTGCGCCCCGACTGGTCACCGCTGGCCCGGCAGTTCcagc                                                                                              <  6:467946/134‑1 (MQ=255)
                                cccGCGGCCCCGCGGCGGGCTGCACGAGGGGCTATGCGGGGGGGGGGCTGGGGGCGGCGGGGGCTTATGAATTGGTATCCACGGGCCTGGTGGCGGGGGGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcagc                                                                                              <  2:496988/134‑1 (MQ=255)
                                cccGCGCCCCCGCCGAGGCCAGCAAGACGCGCTAGGCCGGCCGGGGCCCGCGGGCCGCCGGGCCTCAAGAACTGGTTAACACGGGCCGGGAGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCGGTTCcagc                                                                                              <  1:162246/134‑1 (MQ=255)
                                       cACCGACGCGGGCAGCAAGACGCGCTAGGCCGGCCTGGGGCAGCGGGCCGACGGTGCTGACTAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAAct                                                                                       <  8:257471/135‑1 (MQ=255)
                                       cACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAAct                                                                                       <  8:142987/135‑1 (MQ=255)
                                        aCCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGCCTGGTCACCGCTGGCCCGGCAGTTCCCGCAGGAAct                                                                                       >  4:4270/1‑134 (MQ=255)
                                           gacgaGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGAGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGCCTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                   >  6:152695/1‑135 (MQ=255)
                                              gtgggCAGCAACACGCGCTAGGCCGGCCTGGGGCCGCGGGCCGACGGTGCTGAAGAACGGGTTTACACGGGCCTGGCGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGc                                                                                <  4:3414/133‑1 (MQ=255)
                                              gcgggCTGCAAGACGGGCTAGGCGGGCCGGGGGCCGCGGGCCGACGGTGCTCAAGAAATGTTTAACACGGGCCTGGAGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGc                                                                                <  6:517112/133‑1 (MQ=255)
                                              gaGGGCAGCAAGACGCGCTATGCCGGCCTGGTGCCGCGGGCCGACGGGGTTGAAGAAATGGTTTACAAGGGCCTGGAGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGc                                                                                <  8:134083/135‑1 (MQ=255)
                                                    agctataagGGCTAGGCGGGCCTGGTGCAGCGGGCCGACGGTGCTGAATAAATGTTTTACAAGGGCCTGGAGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgtgtg                                                                          <  6:132018/129‑1 (MQ=255)
                                                          acgggcTATGCCGGCCGGGGCCCGCGGGCCGCCGGGGCTGAAGAAATGTTTTCCATGGGCGGGGCGTGGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGc                                                                    <  6:507977/131‑1 (MQ=255)
                                                          acgcgcTTTGCGGGCCTGGGCCCGGGGGCCGACGGGGCTGAAGAAAGGGTTTACACGGCCGTGGAGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGc                                                                    <  3:26456/133‑1 (MQ=255)
                                                          acgcgcTATGCCGCCCGGGGGCAGCGGGCCGACGGTGCTGAAGAAATGGTTAACAAGGGCCTGGAGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGc                                                                    <  1:333368/133‑1 (MQ=255)
                                                               cTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGGGCGCCCCGACTGGTCACCGCTGGCCCGGCCGGTGCCGCAGGGCCTCTACGGCCGTGTGTTGCGCcgcgc                                                               >  4:514485/1‑134 (MQ=255)
                                                                tctGCGGGCGTGGGGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAg                                                              <  7:304614/133‑1 (MQ=255)
                                                                          tGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGGCGCGAGCCCGACCGTg                                                    >  4:154722/1‑135 (MQ=255)
                                                                          gggTGCCGGGGGCCGACGGTGCTGAAGAAATGGTTTACACGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTg                                                    <  4:417881/134‑1 (MQ=255)
                                                                                 gCGGGCCGACGGTGCTGAATAATTGGTTTACACGGGCCTGGAGGCGGGGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGt                                             <  7:399681/135‑1 (MQ=255)
                                                                                   gggCCGAGGGTGCTTAATAATTGGTTTACAAGGGCCTGGCGTGGGGGCGCCCCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGt                                             <  7:148005/133‑1 (MQ=255)
                                                                                   ggcccGAGGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGGCGGGGCGCACCGACTGGTCACCGCGGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTcc                                           <  8:249989/132‑1 (MQ=255)
                                                                                    ggCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGGTGCGCAGCGCGGCCGACCGGGATTTTGTCcg                                          >  8:520806/1‑135 (MQ=255)
                                                                                      ccGACGGTGCTCAAGAAATGTTTAACACGGGCCGGGAGGCGGGGCCCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCcgcg                                        <  5:196612/135‑1 (MQ=255)
                                                                                           ggTGCTCAACAAATGGTTAACACGGGCCGGGCGCGGGGGCGCCCCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTAt                                   <  1:30271/135‑1 (MQ=255)
                                                                                             tGCTGAACAAAGGGTTCACAAGGGCCTGGAGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGt                                 <  1:416088/135‑1 (MQ=255)
                                                                                               cttaataaaTGTTTTACCTGGGCTTGGTGGCGGGGCCCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTAc                               <  7:196668/129‑1 (MQ=255)
                                                                                                            ttACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGGGTGTTGCGCCGCGCGCCCGACCGGGATTTTGTGCGCGAGTATGTTCGGCGCCCGGTggc                  >  2:467432/1‑135 (MQ=255)
                                                                                                                     ccTGGAGTCGGGGTGCACCGCCTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCa         <  8:86301/135‑1 (MQ=255)
                                                                                                                     ccTGGAGGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCa         <  4:429530/135‑1 (MQ=255)
                                                                                                                            gcGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGaa  <  2:296668/134‑1 (MQ=255)
                                                                                                                                |                                                                                                                                  
GGTGCACTACCGGCGCTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAA  >  ACB122/2725511‑2725769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑