Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,515,1120TG48.9% 13.9 / 17.6 45intergenic (+36/+365)CJ019_03333/vldHhypothetical protein/Validoxylamine A 7'‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (22/1);  new base G (22/0);  total (44/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCTTCGCTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAAGCGCAGCGCCTTGCAACGGCCGCTGGATCAGGCGCC  >  ACB122/3514979‑3515241
                                                                                                                                     |                                                                                                                                 
ggCTTCGCTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACACCGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATCACCTGCCCCGGCgttt                                                                                                                                  >  7:391722/1‑135 (MQ=255)
      gCTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCgtttcaggt                                                                                                                             >  6:273095/1‑132 (MQ=255)
       cTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTCCAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCgtgtcaggtt                                                                                                                            >  1:306038/1‑134 (MQ=255)
                cgccAAGGCCTTGCGCCGGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGt                                                                                                                  <  2:275533/132‑1 (MQ=255)
                   cAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACTCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTGATGTTGAAAGACGGTGGc                                                                                                               >  5:455320/1‑135 (MQ=17)
                    aaGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACACGGCGTTTAAGTTTCAACTTCGGTGGcc                                                                                                              >  6:373205/1‑135 (MQ=255)
                     aGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGccc                                                                                                             >  8:340725/1‑134 (MQ=255)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGCACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTGAGTTTGAACCTCGGTGGCCCtctc                                                                                                         >  8:397904/1‑135 (MQ=18)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGAACACCGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATACCCTGACCCGGCGTGTCAGGTTCGACGTCGGTGGCCAtctc                                                                                                         >  8:3030/1‑135 (MQ=17)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTGAGTTTCAACCTCGGTGGCCCtctc                                                                                                         >  7:57765/1‑135 (MQ=255)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGGTTCAACGTCGGTGGcccactc                                                                                                         >  5:232595/1‑130 (MQ=255)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGGTTCAACGTCGGTGGCCAtctc                                                                                                         >  3:508726/1‑135 (MQ=255)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTGAGTTTCAACGTCGGGGGGCCtctc                                                                                                         >  5:326889/1‑135 (MQ=18)
                         cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCCACGTCGGTGGCCAtctc                                                                                                         >  8:192656/1‑135 (MQ=255)
                           tGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTGAGTTTGAACGGGGGGGGGCCTCTcgc                                                                                                       >  3:23021/1‑135 (MQ=18)
                                    ccGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCACTTTCAACCTCGGTGGCCATCTCGCGCGGCtgtg                                                                                              >  2:104183/1‑135 (MQ=255)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGATCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCCTCTCGCGCGGCTGTgc                                                                                             >  6:401538/1‑135 (MQ=255)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTGAACGTCGGTGGCCATCTCGCGCGGCGGTgc                                                                                             >  5:380746/1‑135 (MQ=255)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCCTCTCGCGCGGCGGTgc                                                                                             >  8:449490/1‑135 (MQ=255)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTgc                                                                                             >  4:320549/1‑135 (MQ=255)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTGAGTTTCAACGACCGTGGCCCACTCGCGCGGCggtgg                                                                                             >  1:414575/1‑134 (MQ=18)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCCGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTgc                                                                                             >  2:516979/1‑135 (MQ=255)
                                     cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCCACGTCGGTGGCCCTCTCGCGCGGCTGTgc                                                                                             >  3:150803/1‑135 (MQ=255)
                                          tGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGGCCTCTAGCGCGGCGGTGcgcgcg                                                                                        >  1:423328/1‑135 (MQ=21)
                                          tGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTAAAAGTCGGTGGCCAAATCGCGCGGCTGTGcgcccg                                                                                        >  3:104988/1‑135 (MQ=21)
                                                    gaACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTGAACCGAGGTGGGCATAACGCGCCGCAGTGGGCGGgccgccgggtg                                                                              >  5:32559/1‑130 (MQ=17)
                                                         acGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTAGCGCGGCTGTGCGCGCGCCGCCTGTTAGGTGa                                                                         >  1:8151/1‑135 (MQ=255)
                                                             cACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCg                                                                      >  8:17202/1‑134 (MQ=255)
                                                                         tGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCCACGTCGGGGGCCCTCTCGCGCGGCTGTTCGCGCGGCGCCTGTTGGGTGCCCGCCAGGTGCGttt                                                          >  1:1483/1‑134 (MQ=25)
                                                                         tGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCTGTTCGGTGCCCGCCAGGTGCgggt                                                          >  1:10606/1‑131 (MQ=25)
                                                                            cTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAg                                                      >  3:125815/1‑135 (MQ=255)
                                                                                   gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGcc                                               >  8:353608/1‑135 (MQ=255)
                                                                                   gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGcc                                               >  4:248258/1‑135 (MQ=255)
                                                                                   gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCTGGTAGGGGACCGCCAGGTGGGGTTCAAGCGACg                                                 >  4:528891/1‑133 (MQ=25)
                                                                                   gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGGGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCGGGTCGGTGACCGCCAGTTGCGTTTCAAGCgccgcc                                               >  1:322688/1‑135 (MQ=37)
                                                                                   gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGGCGGTGGCCCTCTCGCGCGGCGGTGCGCGCGGCGCCGGTTAGGTGACCGCCCGTTGCGGTTCAAGCgccgcc                                               >  2:86291/1‑135 (MQ=25)
                                                                                   gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTAAAAGTCGGTGGCCATCTATCGCGGCGGTGCGCGCGCCGCCGGGTCGGTGTCCGCCCGTTGCGTTTCAACCgccgcc                                               >  5:440405/1‑135 (MQ=25)
                                                                                        gACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTGAGTTTAAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGGCCGCCAGTTGCGTTTGAAGCGCCGCCtggg                                           >  2:49837/1‑133 (MQ=37)
                                                                                        gACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGGCGGTGGCCCTCTCGCGCGGCTGTGCGCGCGCCGCCTGTTCGGTGCCCGCCAGTTGCGGTTCAAGCGACGCCggggg                                          >  6:257992/1‑133 (MQ=37)
                                                                                                 aGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCGGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAagcgca                                 >  8:55396/1‑135 (MQ=255)
                                                                                                      agaagTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTGAACGTCGGTGGCCCTCTCGCCCGGCGGTGCGCGCGACGCCTGGTCGGGGGCCGCCCGTTTCGGTTCAAGCGCCGGCGGGTGGAAAgcgccgcgcc                            >  4:243707/1‑135 (MQ=25)
                                                                                                              cGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAAGCGCCGCGCCTTGCAACg                    >  7:92211/1‑135 (MQ=255)
                                                                                                              cGGTGGATAACCTGACCCGGCGTGTCAGTTTACACCGCGGTGGCCCTCTCGCGCGGCTGTGGGCGCGCCGCCGGGTCGGTGACCGCCACTTGCGTTTCAAGCGCCGCCGGGTGGATAGCGCCGCGCCCTGCCAcc                    >  2:451399/1‑134 (MQ=255)
                                                                                                                gTGGATAACCTGACCCGGCGTTTGAGTTTGAACGTCGGTGGGCATAAAGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCACTTTCGTTTAAAGCGACCCCGGGTGGAAACCCCAGCGCCGTGCAACGGc                  >  8:258599/1‑135 (MQ=255)
                                                                                                                   gATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGCCGCCGGGTGGAAAGCGCAGCGCCATGCAACGGCCGc               >  3:199115/1‑135 (MQ=255)
                                                                                                                     tAACCTGACCCGGCGTTTGAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCGGTTCGGTGACCGCCAGTTGGGGTTGAAGCGACGCCTGGTGGTAAGCGCAGCGCCTTGGAACGGCCGCTg             >  2:460990/1‑135 (MQ=255)
                                                                                                                              ccGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCCCGGCTGTGCGCCCGACGCCTGTTCGGTGCACGCCCGTTGCGTTTCAAGCGCCGCCTGGGGGAAAGCGCAGCGCCTTGCAACCGCCGCTGGATCAGgcg    >  8:447132/1‑135 (MQ=255)
                                                                                                                                ggCGTGTCAGTTTCAACGTCGGTGGCCCTCTCGCGCGGCTGTGCGCGCGCCGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGCCGCCTGGTGGAAAGCGCAGCGCCTTGCAACGGCCGCTGGATCAGGCGcc  >  8:346263/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GGCTTCGCTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAAGCGCAGCGCCTTGCAACGGCCGCTGGATCAGGCGCC  >  ACB122/3514979‑3515241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑