Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,641,8260AC41.2% 53.4 / 17.7 51Q36P (CAG→CCG) CJ019_02499hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (5/25);  new base C (20/1);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.28e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCC  >  ACB143/2641693‑2641954
                                                                                                                                     |                                                                                                                                 
ttGCGCCCCGCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGCCCCGCCAg                                                                                                                                  >  1:149933/1‑135 (MQ=255)
ttGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCa                                                                                                                                   >  6:86266/1‑134 (MQ=255)
      cccccGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGGCCGCCcgcgcggcc                                                                                                                           >  3:355653/1‑133 (MQ=255)
      cccccGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGcgcccg                                                                                                                            >  5:229053/1‑130 (MQ=255)
      cccccGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCGCGAGc                                                                                                                           >  4:52224/1‑136 (MQ=255)
      cccccGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGGCCACCTCGTGGCCCGCCCGCTGGAGc                                                                                                                           >  4:315465/1‑136 (MQ=255)
           gTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCACGCGCggggg                                                                                                                      >  4:165598/1‑135 (MQ=255)
                 aGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTTCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGcc                                                                                                                 <  6:272736/135‑1 (MQ=255)
                 aGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGcc                                                                                                                 <  1:222615/135‑1 (MQ=255)
                     cTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTc                                                                                                             <  4:172761/135‑1 (MQ=255)
                     cTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCg                                                                                                            <  4:9660/136‑1 (MQ=255)
                        cAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCGCGCCCTGGGCGTGCCGGGCggc                                                                                                          >  1:58262/1‑133 (MQ=255)
                            tGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTAc                                                                                                     <  4:205705/136‑1 (MQ=255)
                                   cccTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTc                                                                                                                               >  1:157267/1‑103 (MQ=255)
                                   cccTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTc                                                                                                                               <  2:157267/103‑1 (MQ=255)
                                   cccTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCGCGAGCGGGGCGGGCCGGGGGCCCGGACATtgtg                                                                                               >  7:146965/1‑135 (MQ=255)
                                         ctgGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTggc                                                                                           <  1:6259/133‑1 (MQ=255)
                                         ctgGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTggc                                                                                           <  5:41382/133‑1 (MQ=255)
                                             tCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCctgc                                                                                    <  3:349361/136‑1 (MQ=255)
                                             tCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCct                                                                                      <  5:21255/134‑1 (MQ=255)
                                                    aTGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCACGACCTAGGCCGGCCGGGCGACCGAGCTTTTTGTGGCCGCCGCCTGcccc                                                                              >  2:370240/1‑133 (MQ=255)
                                                     tGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGGCGACCGTACTTTGTGTGGCGGCCTGCGTCCAg                                                                              >  5:74916/1‑134 (MQ=255)
                                                     tGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCACGACCTGGGCGTGCCCGGCGCCCGTACTTTGTTTGGGGGCCGGCTGCCAGc                                                                             >  2:132462/1‑135 (MQ=255)
                                                     tGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCTCGAGCGGGGGGGGGCGGTCGCCCGGACTTTTTGTGGCGGCCTGCTGCCAGc                                                                             >  6:196858/1‑135 (MQ=255)
                                                        cTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTc                                                                           <  7:92851/134‑1 (MQ=255)
                                                          aTCAACTGAGCGCGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGGCCACCTCGGGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGcc                                                                       <  3:211178/136‑1 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGCGCGGGGCGTGCCGGGCGCCCGGACTTTGTGTGGCGGCCGGCTGCCAGCTCCGCcgc‑g                                                                   >  6:214215/1‑135 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGCGGGGGGCGTGGCGGGGGGCCCGACTTTGTGTGGCGGCCGGCGGCCAGCGCCGCcgc‑g                                                                   >  1:23548/1‑135 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCGCCGCTCGAGCTGGGGGGGGCGGTGGAGCGGACTTTTTGTGGCGGGCTGGTGGCCGCTGATCcgc‑g                                                                   >  1:14456/1‑135 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCACCCGCGGGGGGGGCCGGGCGCCCGGACGTTTTGTTGAGGCCGGGGGCCCGCTCACCcgc‑g                                                                   >  6:378857/1‑135 (MQ=255)
                                                              aCTGATAGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCACCACCACCTGGGCGTGCAGGTCGCACGGACCTTGTGTTGCGGCCAGCTGCCAGCACAGCcgcc                                                                    >  2:57973/1‑134 (MQ=255)
                                                                  aTCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGCGCGGGGGGGGGCGGGGGGCCGGACTTTGTGTGGGGGGCGGCGGCCCGCTCCGCCCC‑GGTgg                                                               >  8:44760/1‑135 (MQ=255)
                                                                     gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCGGGGCGGGCCGGTGGGCCGGACTTTGTGTGGCGGGCGGCGGCCAGCACCGCCGC‑GGTGGGCg                                                            >  7:222543/1‑135 (MQ=255)
                                                                     gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCGCGAGCGGGGGGTGGCGGGCGCCCGGACTTTGTGTGGCGGGCTGCTGGCCGCGCAGCCGC‑ggtggtgg                                                            >  7:12271/1‑133 (MQ=255)
                                                                             cgaGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCcg                                                   <  4:69353/136‑1 (MQ=255)
                                                                              gaGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCcgc                                                  <  3:35088/136‑1 (MQ=255)
                                                                               agcagcTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCTGGGCGTGCCGGTCGACCGGACTTTGTGTGGCGGCCGGCTGCCCGCTCAGCCGC‑GGTGGTGGGGGGTGCcgc                                                  >  1:257491/1‑135 (MQ=255)
                                                                               agcagcTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCAGCTAGCGCTGGGCGAGGCGGTGGCCAGTAATTTTTGTGTCGGCCTGCTGCCCGCTCCGCCGCGGTTGTTCGGGGTGGCcg                                                   >  2:53823/1‑135 (MQ=255)
                                                                                               cGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGAt                                 <  4:21274/136‑1 (MQ=255)
                                                                                                gCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGAt                                 <  3:11019/135‑1 (MQ=255)
                                                                                                 ccTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCGCGCGCGGGGCGGGGCGGGGGGCCGGACGTTGTGTTGGGGCCTGCGGCCCGCGCCGCCGC‑GGGGGGGGGGGGGGCCGCGGGCGGTCGTCCGCGaag                                >  8:48857/1‑133 (MQ=255)
                                                                                                  cTGGCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGTGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                               <  6:360998/135‑1 (MQ=255)
                                                                                                  cTGCCAGGACAGCCGCGTCCACCTCGGGGCCCGCCAGCTCGAGCTGGGCGGGCCGGTCGACCGTACTGTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                               <  2:270635/135‑1 (MQ=255)
                                                                                                     ccAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCTGGGCGTGCCGGGCGCCCGGACTTTGTGTGGCGGCCTGCTGCCCGCTCAGCCGC‑GTTGGGCGGGGGTGCCGCGCTCGCTCTACCGCGAGGGGCg                            >  5:316566/1‑135 (MQ=255)
                                                                                                      cAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGcc                          <  3:228476/136‑1 (MQ=255)
                                                                                                         gAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCtt                        <  2:157966/135‑1 (MQ=255)
                                                                                                           caGCCGCGGCGACCTCGGGGCCCGCCCGCTGGAGCGGGGCGTGCCGGTCGACCGTAGTTTGTGTGGCGGCGTGCTGGCAGGTGAGGCGC‑GTTGGTCGGGGGTGCCGCTATCGATCTACTGCGATGGTCGCCTTtgt                     <  3:85298/135‑1 (MQ=255)
                                                                                                           aacgcgcgTCCTCCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGGCGGTCGTGCGTACTTTGTGTGGCGGCGTGCTGGCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTAGCGCGATGGTCGCCTTtgt                     <  3:85294/132‑1 (MQ=255)
                                                                                                                gcgTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCt                <  3:105649/136‑1 (MQ=255)
                                                                                                                   tCCACCTCGTGGCCCGCCAGCTCTAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCtgt              <  1:243833/135‑1 (MQ=255)
                                                                                                                        cTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgt         <  2:401083/135‑1 (MQ=255)
                                                                                                                            tGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACGTTGTGTTGCGGCCTGCTGCCCGCGCAGCCGC‑GGGGGTGGGGGGTGCCGCGCGCGCTCTACCGCGATGGTGGCCTTGGTGAGGTGTGCCGGGCCa     >  6:381492/1‑135 (MQ=255)
                                                                                                                               cccGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATcc  <  6:222249/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
TTGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGC‑GTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCC  >  ACB143/2641693‑2641954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑