Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,138,3500TG34.1% 44.4 / 10.3 44F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (14/15);  new base G (15/0);  total (29/15)
Fisher's exact test for biased strand distribution p-value = 4.86e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTG  >  ACB143/3138221‑3138481
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acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGt                                                                                                                                >  7:65031/1‑135 (MQ=255)
     aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggc                                                                                                                           >  5:341738/1‑135 (MQ=255)
     aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGGTgggcg                                                                                                                          >  3:357711/1‑136 (MQ=255)
      ttCGTCCTGCGGCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                          <  2:294514/135‑1 (MQ=255)
      ttCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                          <  8:29083/135‑1 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTaggcggggt                                                                                                                      >  2:327301/1‑132 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCt                                                                                                                      >  8:352604/1‑135 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCt                                                                                                                      >  2:297869/1‑135 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGGCt                                                                                                                      >  8:4686/1‑135 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCt                                                                                                                      >  2:386450/1‑135 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGCGGccg                                                                                                                      >  1:254317/1‑134 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGCGGccg                                                                                                                      >  2:181866/1‑134 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGGGGccg                                                                                                                      >  7:136971/1‑134 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGCCt                                                                                                                      >  7:18422/1‑135 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGGTGGGGGGCCt                                                                                                                      >  8:155686/1‑135 (MQ=255)
          gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGGTGTTGGGGGGccg                                                                                                                      >  6:255773/1‑134 (MQ=255)
                 tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAg                                                                                                               >  2:57013/1‑135 (MQ=255)
                   tGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCgg                                                                                                            <  3:74944/136‑1 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGGCGGGTTGAGCGgcagcc                                                                                                       >  3:83439/1‑135 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGCCTGGTTCAGCGgcagc                                                                                                        >  5:153758/1‑135 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTTTTGGGCGGCCCGGTTGAGCcgccgc                                                                                                        >  7:65921/1‑135 (MQ=255)
                         ccATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                      <  4:371423/136‑1 (MQ=255)
                             cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCGGGGTCAGCGGCAGCaccccc                                                                                                  >  4:303152/1‑136 (MQ=255)
                             cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGGGGGCTGGTTCAGCGGCAGcaccac                                                                                                   >  8:38486/1‑135 (MQ=255)
                             cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTGAGCGGCAGCcccacc                                                                                                  >  3:65356/1‑136 (MQ=255)
                               gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGGGGGCGGGGTCAGCGGCAGCaccaccc                                                                                                 >  6:104739/1‑134 (MQ=255)
                                       ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCgg                                                                                          >  7:3588/1‑134 (MQ=255)
                                          gAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCgag                                                                                      <  6:97548/135‑1 (MQ=255)
                                            ggcggcTAATGCCGCTGGCCTGGCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAt                                                                                    <  1:89237/135‑1 (MQ=255)
                                                             gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCCCCACCGGTTCCGGCGAGATGCTGCTGTCCGtggtgg                                                                   >  8:216599/1‑135 (MQ=255)
                                                                   cGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt                                                               <  1:47524/133‑1 (MQ=255)
                                                                      tGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                         <  4:191451/136‑1 (MQ=255)
                                                                      tGCGTGGCTACGTGTTCGCCAGCTACCTGAGCAGCGGTCTGTGCGGCAGCCGGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                         <  4:323750/136‑1 (MQ=255)
                                                                       gCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCttt                                                         <  8:371173/135‑1 (MQ=255)
                                                                          tGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGGGGGCGGGGTGAGCGGCAGCACCACCGGTTGCGGGGCGATTGTGCGGTCCGTGGTTGGGCTCGCTTTTGtt                                                     >  3:135411/1‑136 (MQ=255)
                                                                           ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCtt                                                     <  1:386450/135‑1 (MQ=255)
                                                                             cTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                   <  2:267338/135‑1 (MQ=255)
                                                                             cTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                   <  6:135201/135‑1 (MQ=255)
                                                                                               ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCTCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGt                                <  4:302826/136‑1 (MQ=255)
                                                                                               ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGGCTGGTTCAGCGGCCGCCCCCCCCGGTGCGGCGAGCTGCTGCGGTGCGGGGGGGCCAGCGCGTTTGTTGGGGTGATCTTCTCCCcgcgg                                >  4:253650/1‑135 (MQ=255)
                                                                                                         ggTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCTGGTTAAGCGGCAGCCCCACCGGGTGCGGCGAGATTCTGCTGTCCGTGGTGGGCATAGCATTTGTTGGGGTGATCTTCAAACCGCGTCTGCAGccc                       >  6:178799/1‑134 (MQ=255)
                                                                                                                     aGCCTGGCCGCGTTTTGATCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCggcggc           <  7:14803/135‑1 (MQ=255)
                                                                                                                            ccGCGTTTTGTTGGGCGGGCAGGGTCAGCCGCAGCCCCACCGGGTCCGGCGAGATGGTGGTGGCCGTGGTGGCCATCGCTTTTATTGGGGTGTTATTCTCCCACCGTCTGCCGCCGAGCATAGGCGGCCCgcggct   >  4:122647/1‑136 (MQ=255)
                                                                                                                              gcgTTTTGTTCGGCGGCCGGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCGTTTCTTGGGGTGATCTTCTCCCCGCGTCTGCAGCCGAGCATCGGCGGCAAGctgctg  >  7:263158/1‑135 (MQ=255)
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ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTG  >  ACB143/3138221‑3138481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑