Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3260AC53.1% ‑5.8 / 12.3 32S18R (AGC→CGC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (13/2);  new base C (14/3);  total (27/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  ACB143/3440205‑3440456
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gTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGcc                                                                                                                      >  3:264182/1‑136 (MQ=255)
     aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCggg                                                                                                                  >  3:402594/1‑135 (MQ=255)
               gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCGGGCCGCCTGGgtg                                                                                                       >  4:373020/1‑136 (MQ=255)
                cAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTggggg                                                                                                       >  5:382571/1‑133 (MQ=255)
                       cTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCCCAGGCGGCCCCCGGGCCGCCGGGGTGTGGCAgg                                                                                                >  6:63883/1‑135 (MQ=255)
                        tGGAGGCGCGCCAGGCCCTCTACAAGCGCGCGCTCGTGGTGCTGCAGTGAGCGCTTGGGCCCCCCGCCGGGGGGCGGGGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGc                                                                                               <  8:232275/135‑1 (MQ=255)
                         ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCCGGCGGCGGCCGGGCAGCCGGGGTGTGGCAGGCCa                                                                                             >  4:218284/1‑136 (MQ=255)
                                cGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGCAGGCCAACGcc                                                                                        >  1:149589/1‑134 (MQ=255)
                                  cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCCACGccga                                                                                      >  5:84729/1‑132 (MQ=255)
                                   aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCGGGCCAACGCCTATaa                                                                                   >  4:400473/1‑136 (MQ=255)
                                   aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCAGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCCGGCCACCCCCgata                                                                                    >  6:379018/1‑135 (MQ=255)
                                                    agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCACCCCCGATAAGGCGCAGCGCGCTGa                                                                    >  2:236654/1‑134 (MQ=255)
                                                       gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCCACGCCGATAAGGCGCCGCGCGGGGATCAgg                                                               >  4:98774/1‑136 (MQ=255)
                                                       gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCACGCCTATAAGGCGCAGCTCGCTGAGCAgg                                                               >  4:386359/1‑136 (MQ=255)
                                                       gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAg                                                                >  1:10433/1‑135 (MQ=255)
                                                         tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCACCCCGATAATGCGCAGCGCGCTGCTCAGGc                                                              >  8:118066/1‑135 (MQ=255)
                                                         tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGc                                                              >  1:69819/1‑135 (MQ=255)
                                                         tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCACGCCCACCCCTATAAGGCGCCCCTCGCTGATAAGGcc                                                             >  4:192211/1‑136 (MQ=255)
                                                         tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCCCCCCGGCAGCCTGGGTGTTGGAGGCCCACCCCAATAAAGCGCAGCTCGCTGATCAGGc                                                              >  5:101500/1‑135 (MQ=255)
                                                         tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGGGGGGGAGGCCACCCCCGATAATGCGCAGCGCGCGGGTCAGGc                                                              >  2:407137/1‑135 (MQ=255)
                                                         tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCACCGCTGGCGGCGCCCCGGCAGCCTGGGGGTGGGCGGCCCCCCCCGATAAGGCGCAGCGCGCTGGTCAGGc                                                              >  6:171729/1‑135 (MQ=255)
                                                           gAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCGGGCCGCCGGGGTGGGGCAGGCCACCGCCGATAATGCGCCGCTCGCGGATCAGGCCg                                                            >  5:232555/1‑135 (MQ=255)
                                                                    cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCAGCCGGGGGGTGGCAGGCCACCCCCTATAAGGCGCCCCGCGGCGATCACGCCGCGGACttag                                                   >  8:206246/1‑133 (MQ=255)
                                                                          gcgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATTAGGCGCAGCTCGCTGATCAGGCCGATGCCTTCGGCaa                                               >  8:46739/1‑133 (MQ=255)
                                                                            gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGGGGCGCCCGGGCCGCCGGGGGGTGGCACGGCAACCCCTCAAAGGCGCCGCGCGCGGCTCAGGCCGCTGACGTCGGCGAggcc                                           >  6:286988/1‑132 (MQ=255)
                                                                            gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGGCGGCCCACCCCTCTAAGGCGCCGCGCGCGGCTCAGGCCGCGGCCGTCGGCAAGCAg                                           >  6:152701/1‑135 (MQ=255)
                                                                            gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCGGCCTGGGTGTGGCAGGCCAACCCCAAAAAGGCGCAGCTCGCGGAGCAGGCCGATGACGTCGGCCCGCAg                                           >  7:327344/1‑135 (MQ=255)
                                                                             cgggggCCCCCGGCGGGGGCGGCGGGGCGGGGCGGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt                                          <  1:407137/132‑1 (MQ=255)
                                                                                                cGCCGTGGCGCTGCTGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  8:41625/135‑1 (MQ=255)
                                                                                                  ccGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGCAGGCCAACGCCGATAAGGCGCAGCGCGCGGCTCAGGCCGCTGGCTTCGGCAAGCAGTTGGGGGTGAAGGAGcgcgcgg                     >  6:198475/1‑134 (MQ=255)
                                                                                                                    ccgccAGCGCCGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa  <  4:300364/136‑1 (MQ=255)
                                                                                                                     cgccCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa  <  6:133231/135‑1 (MQ=255)
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GTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  ACB143/3440205‑3440456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑