Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,423,0830TG38.0% 48.4 / 10.6 50V51G (GTG→GGG) fabG_63‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base T (21/10);  new base G (0/19);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 7.40e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACA  >  ACB143/4422948‑4423214
                                                                                                                                       |                                                                                                                                   
tGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGt                                                                                                                                     >  3:294666/1‑136 (MQ=255)
  ttCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTg                                                                                                                                    <  3:189738/135‑1 (MQ=255)
          cGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACg                                                                                                                             >  7:64924/1‑134 (MQ=255)
               gCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCcatca                                                                                                                       >  6:71821/1‑135 (MQ=255)
                   gATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTgc                                                                                                                   >  8:236317/1‑135 (MQ=255)
                    aTTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTgcc                                                                                                                  >  2:396449/1‑135 (MQ=255)
                         cTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGCCGGGGGCCAGCAGGGGGCCGACGCCATCATTGCCGCcggc                                                                                                            <  4:84634/136‑1 (MQ=255)
                          tttGTTTCCGGCGCCCGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGAAAGCTTGCCGGGTGCCCGCAGGTGGCCGACGCCATCATTGCCGCcggc                                                                                                            <  6:241369/135‑1 (MQ=255)
                          tttGTTTCCGGCGCCAGCGGGGGCAGCGGCGCGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCCGCCGCAAGCTTCCCGGGGGCCCGCAGGTGGCCGACGCCATCATTGCCGCcggc                                                                                                            <  2:95569/135‑1 (MQ=255)
                          tgtGTTGCCGGCGCCCGCCGGGGCAGCGGCGAGGCCATCGCCCACGTGCGCGCGCAGCACGGGGCCCATGTGATCGTGTCCAGCCGCAAGCTTGACGGGGGCCCGCCGGGGGCCGCCGCCATCATGGCCGCcggc                                                                                                            <  7:276092/133‑1 (MQ=255)
                           ttGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCcggcg                                                                                                           >  7:67532/1‑135 (MQ=255)
                                tCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAgg                                                                                                     >  3:13093/1‑136 (MQ=255)
                                tCCGGCGCCAGCCGTGGCATCGGCGAGGCCATAGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCACGTGGCCGACGCCATCATTCCCGCCGGCGGCaac                                                                                                      >  1:179879/1‑134 (MQ=255)
                                 ccGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTGGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCCGCCGCACGCTTGACGGGGGCCCGCCGGGGGCCGACGCCACCATTGCCGCCGGCGGCAAgg                                                                                                     <  4:182292/135‑1 (MQ=255)
                                  cGGCGCACGCCGTGGCAGAGGCGCGGCCTTCGCCAACTTGCGCGCGCAGCATGGGGCCCTTGTGATCGTTTCCAGCCGCACGCTTGACGGGTGCCCGCAGGGGGCCGCCGCCATCATTGCCGCCGGCGGCAAgg                                                                                                     <  6:174054/134‑1 (MQ=255)
                                            cGTGGCATCGGCGCGGCCAGCGCCCACTTGCGCGCGCAGCAAGGGGCCCATGTGAGCGTTTCCCGCCGCAAGCTTGAGGGGTGCCCGCAGGGGGCCCCCGCCATCATGGCCGCCGGGGGCAAGGCCACGGCtgtg                                                                                          <  2:28109/135‑1 (MQ=255)
                                                   gcGGCGCGGCAAGCGCCCACTTGCGCGCGCAGAATGGGGCCCTTGTGAGGGTTGCCAGCCGCACGGTTGAGGGGTGCCAGCTGGGGGCCGACGCCATCATGGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGcc                                                                                   <  8:27800/134‑1 (MQ=255)
                                                       cgcggCCATCGCCAACTTGCTCGCGCAGCAAGGGGCCCATGTTAGCGTTTCCCGCCGCACGCTTGCCGGGGGCCAGCAGGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACAt                                                                               <  8:124144/132‑1 (MQ=255)
                                                        gAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACAtt                                                                              >  5:84616/1‑135 (MQ=255)
                                                           gCCAGCGCCCACTTGCGCCCGCAGCAAGGGGCCCATGTGAGCGTTTCCAGCCGCAAGCTTGCCGGGTGCCAGCCGGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGt                                                                           <  5:366867/135‑1 (MQ=255)
                                                               tCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCCCGGGTGTGGCCTGCCACATTGGGGAGc                                                                       >  7:138352/1‑135 (MQ=255)
                                                                cGCCCACTTGCTCGCGCACCATGGGGCCCAGGTGAGCGTTACCAGCCGAACGCTTGCGGGGGCCCAGCAGGTGGCCGCCGCCATCATTGCCGCGGGGGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTg                                                                     <  3:296786/136‑1 (MQ=255)
                                                                  ccccgttGCCCGCGCAGCATGGGGCCCTTGTGAGGTTTCCCCCCCGCACGGTTGCGGGGGGCCCGCGGGGGGCCGCCCCAATATTTGCCGCGGGCGCCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGa                                                                   <  4:181932/131‑1 (MQ=255)
                                                                   ccACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCCCCGCTGTGGCCTGCCACATTGGTGAGCTGGa                                                                   >  5:30482/1‑135 (MQ=255)
                                                                   ccACTTGCTCGCGCAGCAAGGGGCCCAGGTGCTCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCCGGTGTCCGACTCCATCATTGACGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGGTAGATGGa                                                                   >  4:288031/1‑135 (MQ=255)
                                                                     aCTTGCTCGCGCAGCATGGGGCCCATGTGATCTTTTCCAGCCCCAAGCTTGACGGGGGCCAGCTGGTGGCCGACGCCATCTTTGCCGCGGGGGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAAc                                                                 <  6:200391/135‑1 (MQ=255)
                                                                         gCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGAt                                                             >  2:193955/1‑135 (MQ=255)
                                                                         gCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCGGGAACAGAt                                                             >  5:340542/1‑135 (MQ=255)
                                                                             gcgcAGCAAGGGGCCCATGTGATCGTTTCCCGCCGCAAGCTTGAGGGGTGCCAGCAGGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTcag                                                         <  8:92373/135‑1 (MQ=255)
                                                                               gcagcaAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCCCGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGAGTcagca                                                       >  8:89464/1‑135 (MQ=255)
                                                                               gcagcaAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTcagca                                                       >  2:379628/1‑135 (MQ=255)
                                                                                    aaGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGgtgt                                                  >  8:27289/1‑135 (MQ=255)
                                                                                             ttgtgATGGTTTCCAGCCGCAAGGTTGACGGGTCCCCGCAGGGGGCCGACGCCATCATTGCCGCGGGGGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCa                                         <  2:108939/134‑1 (MQ=255)
                                                                                                tgttCGTTACCCGCCGCACGCTTGCGGGGTGCCCCCCGGGGGCCGCCGCCATCTTTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTc                                      <  4:113220/132‑1 (MQ=255)
                                                                                                tgATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTc                                      >  8:237716/1‑135 (MQ=255)
                                                                                                tgATCGTATCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAACGCCACGGCTGTGGCCTGCCACATTGCTGAGCAGGAACAGATTCAGCAGGTGTTCGCAGGCATTc                                      >  2:185049/1‑135 (MQ=255)
                                                                                                  agcGTTCCCCGCCCCACGCTTGCCGGGTGCCAGCAGGGGGCCGACGCCATCATTCCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgc                                    <  3:23685/133‑1 (MQ=255)
                                                                                                  aTCGTTCCCAGCCGCAAGCTTGACGGGTGCCAGCAGGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgc                                    <  4:296928/135‑1 (MQ=255)
                                                                                                   tCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCCGGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgc                                    <  8:248227/134‑1 (MQ=255)
                                                                                                   tCGTTGCCAGCCGCACGCTTGACGGGTGCCAGCTGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgc                                    <  2:86059/134‑1 (MQ=255)
                                                                                                   tCGTTCCCACCCCCACGCTTGCCGGGGCCCCGCAGGGGGCCTCCGCCATCATTGCCGCGGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTcgc                                    <  6:234964/134‑1 (MQ=255)
                                                                                                     gTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGa                                  >  1:191922/1‑134 (MQ=255)
                                                                                                      tttCCAGCCGCAAGCTTGACGGGTGCCAGCAGGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACa                               <  4:279395/136‑1 (MQ=255)
                                                                                                       ttgacgCCGCATGTTTGACGGGGGCCTGCCGGTGGCCGACGCATTAATTGCGGCCGGGGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACa                               <  4:95004/130‑1 (MQ=255)
                                                                                                          cAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAACGCCACGGCTGTTGCCTGCCACAATGGTGAGCTGGAACAGAATCAGCAGGAGTTCGCCGGCATTCGCGAACATTTc                           >  3:372839/1‑136 (MQ=255)
                                                                                                                   gCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACt                   >  6:39426/1‑135 (MQ=255)
                                                                                                                        cggggTGCCTGCTGGTGCCCGACGCCATCATTGCGGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGAc               <  1:296413/132‑1 (MQ=255)
                                                                                                                        cggggTGCCCGCTGGGGGCCGACCCAATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGAc               <  8:180181/132‑1 (MQ=255)
                                                                                                                               cccgcagGGGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGt       <  6:191649/132‑1 (MQ=255)
                                                                                                                                cagccgGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTc      <  7:236317/135‑1 (MQ=255)
                                                                                                                                    tggTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTcaaca  <  5:204051/134‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGGTGTTCGCCGGCATTCGCGAACAGTTCGGGCGACTGGACGTGCTGGTCAACA  >  ACB143/4422948‑4423214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑