| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,251,184 | 0 | C | A | 56.2% | 7.8 / 16.1 | 16 | intergenic (‑64/+80) | CJ019_04948/fabV | hypothetical protein/Enoyl‑[acyl‑carrier‑protein] reductase [NADH] |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base A (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGAC > ACB143/5251057‑5251275 | cgAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGa < 3:317877/136‑1 (MQ=255) gAGGCTGTATCCGTCGGCCGCGGGTGGGCGAAATGGTGTAGCGGGTTTGATGAGTCTACTCATGGCGAATGTCCTGTTGTTGTTCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGTGTGa < 6:97614/135‑1 (MQ=255) gAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTGGTAGCGGGTgg > 1:327692/1‑134 (MQ=255) ccGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCATAGCGGGGGTAcccccgcc > 2:92295/1‑129 (MQ=255) ggCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTATCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAggg < 4:220803/136‑1 (MQ=255) ggCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAggg < 4:81366/136‑1 (MQ=255) ccGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAAACCCCTCCGACAgggg > 8:44157/1‑135 (MQ=255) cgGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGgag < 8:27171/135‑1 (MQ=255) gTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTAGCGGGGGAACCCCCTCCGACAGGGGGAGCACCGGTGGGTGCGGGCTTGCCcg > 5:317093/1‑135 (MQ=255) aTGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTAGCGGGTGAACCCGCTCCTACAGGGGGAGAAACTGTGGGAGCGGGCTTGCCCGCGa > 8:103082/1‑133 (MQ=255) aTGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTAGCGGGTGAACCCCCTCCTAAAGGGGGTGAACCTGTGGGGGCGGGGTTTGCCGCGa > 8:210298/1‑133 (MQ=255) aTGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTGATAGCGGGTGGACCCGCGGCTAAAGGGGGTGGACCGGTGGGGGGGGGGTTGGCCGCGAATGGGCCGGGACAg > 1:87887/1‑135 (MQ=255) tgttgttgtGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTAGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTTCCCGCGAAGAGGCCGGTACAGGCTTACCCGAtt > 2:320038/1‑134 (MQ=255) gtgCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTAGCGGGTGAACCCGCTCCGAAAGGGGGAGAACCAGTGGGAGCGGGCTTGCCCCCGGAGAGGCCGGTACAGGCTTACCCCATTAAAGCTCg > 7:258018/1‑135 (MQ=38) cgcCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCAGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGAc > 7:370734/1‑134 (MQ=255) gcCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTAGCGGGTGAACCCCCGCCTACAGGGGGAGAACCGGTGGGAGCGGGCTTGCCCGCGAAGATGCCCGTTCAGGCTTAACCCATTACATCTAGaa > 7:287147/1‑132 (MQ=37) | CGAGGCTGTATCCGTCGGCCGCGGGGTGGCGAAATGGGGTAGCGGGTTGGATGAGGCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGAC > ACB143/5251057‑5251275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |