Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,503	C→G	intergenic (‑512/+1139)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,503	0	C	G	100.0%	81.3 / NA	22	intergenic (‑512/+1139)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/11);  total (11/11)

GCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑A‑‑TC‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT  >  ACB143/5386371‑5386607                                                                                                                                       |                                                                                                                  tcaagcagTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCGTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg                                                                                                                <  4:97687/133‑2 (MQ=255) gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt                                                                                                                 <  7:340722/135‑1 (MQ=255) gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt                                                                                                                 >  2:318237/1‑135 (MQ=255) gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCACGGCCGGCTTGTCGACGACTGCGGTCTCagt‑‑ggt                                                                                                                 >  8:269641/1‑135 (MQ=255) gCCAGCAGTCTAACTAAAAAGGCCTCACAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg                                                                                                                <  4:198214/136‑2 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGCCGGCGGTCTCagt‑‑ggtg                                                                                                                >  4:107832/1‑134 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg                                                                                                                <  2:223091/135‑2 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg                                                                                                                <  6:372559/135‑2 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg                                                                                                                >  5:27011/1‑134 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt                                                                                                                 >  8:253399/1‑134 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt                                                                                                                 >  2:397788/1‑134 (MQ=255)  ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATAGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg                                                                                                                >  3:35307/1‑134 (MQ=255)       agTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtgtacgg                                                                                                           >  4:254812/1‑129 (MQ=255)                                            ttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGcccc                                                                 >  8:102260/1‑135 (MQ=255)                                                    aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGa                                                          <  8:262590/134‑1 (MQ=255)                                                     tAACAAAAATTGATCGAATTCTTTCATTAAATACCCTAATATCACAGCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATc                                                        <  5:32403/135‑1 (MQ=255)                                                         aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                    >  8:110250/1‑135 (MQ=255)                                                                 aTCGAATTCTTTCATTTACGACCCTAATATGACAGCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGAACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                            <  4:185565/135‑1 (MQ=255)                                                                             cATTAAAGACCCTAATATGCCATCTCGGCCGGCTGGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGAAC‑‑AACCC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                  <  1:371333/133‑1 (MQ=255)                                                                              aTTTAAGACCCTAATATCACATCTCGGCCGGCTGGGCGACGACGGCGGTCTCAGG‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                              <  4:366827/136‑1 (MQ=255)                                                                                 tAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑AC‑GGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTg                            <  3:296930/135‑1 (MQ=255)                                                                                          aaTATGACATCTCGGCCGGCTTGTCGACGAAGGCGTTCTCAGT‑‑GGTGTACG‑GTACA‑‑A‑‑ACACCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCATCCTGGGGATTTTTTATTTCTCTGCGGGTGCCAg                  >  4:125691/1‑136 (MQ=255)                                                                                           atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                 <  4:112251/136‑1 (MQ=255)                                                                                            tatGACATCTCGGCCGGCTTGTCGACGAGGGCGGGCTCTGTGGGGT‑‑‑AC‑GGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                 <  7:82993/135‑1 (MQ=255)                                                                                                   tctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                  <  5:369642/111‑1 (MQ=255)                                                                                                   tctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                  >  6:369642/1‑111 (MQ=255)                                                                                                    ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTAC‑‑AACCC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGTGTGCCAGGGCGTGccc         >  8:154/1‑135 (MQ=255)                                                                                                       ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt       <  4:311429/134‑1 (MQ=255)                                                                                                       ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg     >  4:377128/1‑136 (MQ=255)                                                                                                       ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg     >  4:362330/1‑136 (MQ=255)                                                                                                       ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg      >  1:199257/1‑135 (MQ=255)                                                                                                        gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCttcg     >  7:258659/1‑133 (MQ=255)                                                                                                        gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt       <  5:66238/133‑1 (MQ=255)                                                                                                         ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg    >  2:284182/1‑135 (MQ=255)                                                                                                           ggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt  >  2:375146/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                  GCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑A‑‑TC‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT  >  ACB143/5386371‑5386607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑