| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,510 | 2 bp→TA | intergenic (‑519/+1131) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,510 | 0 | A | T | 86.4% | 41.9 / ‑1.3 | 22 | intergenic (‑519/+1132) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (10/9); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | ACB143 | 5,386,511 | 0 | T | A | 95.5% | 62.8 / ‑5.4 | 22 | intergenic (‑520/+1131) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (10/11); minor base C (1/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑A‑‑TC‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT > ACB143/5386414‑5386607 || ttttATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGcccc > 8:102260/1‑135 (MQ=255) aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGa < 8:262590/134‑1 (MQ=255) tAACAAAAATTGATCGAATTCTTTCATTAAATACCCTAATATCACAGCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATc < 5:32403/135‑1 (MQ=255) aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCt > 8:110250/1‑135 (MQ=255) aTCGAATTCTTTCATTTACGACCCTAATATGACAGCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGAACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 4:185565/135‑1 (MQ=255) cATTAAAGACCCTAATATGCCATCTCGGCCGGCTGGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGAAC‑‑AACCC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 1:371333/133‑1 (MQ=255) aTTTAAGACCCTAATATCACATCTCGGCCGGCTGGGCGACGACGGCGGTCTCAGG‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 4:366827/136‑1 (MQ=255) tAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑AC‑GGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTg < 3:296930/135‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGAAGGCGTTCTCAGT‑‑GGTGTACG‑GTACA‑‑A‑‑ACACCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCATCCTGGGGATTTTTTATTTCTCTGCGGGTGCCAg > 4:125691/1‑136 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 4:112251/136‑1 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGAGGGCGGGCTCTGTGGGGT‑‑‑AC‑GGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 7:82993/135‑1 (MQ=255) tctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 5:369642/111‑1 (MQ=255) tctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt > 6:369642/1‑111 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTAC‑‑AACCC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGTGTGCCAGGGCGTGccc > 8:154/1‑135 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 4:311429/134‑1 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 4:362330/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg > 4:377128/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg > 1:199257/1‑135 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCttcg > 7:258659/1‑133 (MQ=255) gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 5:66238/133‑1 (MQ=255) ccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 2:284182/1‑135 (MQ=255) ggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAAA‑‑CC‑CCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt > 2:375146/1‑135 (MQ=255) || TTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑A‑‑TC‑CGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT > ACB143/5386414‑5386607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |