Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,413,5730GC22.6% 103.0 / 11.1 53intergenic (‑439/‑591)lexA_2/CJ019_03236LexA repressor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/12);  new base C (0/12);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.01e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGGATTACACCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAACTACTGTCCTTGAACCCGGGGGTCCAGGCACAAGTGCCTGGGTG  >  ACB143/3413441‑3413703
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aCGGATTACACCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGcca                                                                                                                                >  2:227988/1‑135 (MQ=255)
       acacCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAGAGGCGACCCGaaa                                                                                                                         >  6:192017/1‑136 (MQ=255)
       acacCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAg                                                                                                                         >  8:261067/1‑137 (MQ=255)
       acacCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAg                                                                                                                         >  1:336593/1‑137 (MQ=255)
       acacCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAg                                                                                                                         >  8:214353/1‑137 (MQ=255)
       acacCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAg                                                                                                                         >  6:279130/1‑137 (MQ=255)
       acacCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAg                                                                                                                         >  6:211544/1‑137 (MQ=255)
                aaaCGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTgg                                                                                                                >  8:249019/1‑137 (MQ=255)
                 aaCGAATTATGCAGAGCGTTTAGCGAATGCGGCTGGACAAAAAGGTAATACATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGCCGACCCGAAGGTCTCCTTGt                                                                                                               >  2:146856/1‑137 (MQ=255)
                   cGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGttt                                                                                                             >  8:167095/1‑137 (MQ=255)
                        tATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTccccc                                                                                                        >  8:160780/1‑137 (MQ=255)
                        tATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTccccc                                                                                                        <  7:180789/137‑1 (MQ=255)
                          tGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCag                                                                                                      >  1:240139/1‑137 (MQ=255)
                          tGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCag                                                                                                      >  1:240140/1‑137 (MQ=255)
                          tGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCACAGACAAACGCCGACATAGAAAAAAGGCGACCCGAAGGGCGCATCGTTTcccccca                                                                                                      >  2:180951/1‑135 (MQ=255)
                           ccAGAGCGTTTAGCTAATGCGGCTGGACAAAAAGTTAATCCATTTGAAGCGTCCTGGTGGCTTGAAGTGGCGGCTCCTCAGACACAAAGGCCGACAAAGAAAAACGGCGACCCGAAGGTCGCCTGGTTTCCCCCagt                                                                                                     <  7:327162/136‑1 (MQ=255)
                             agagGGTTTAGCGATTGGTGTGGTAAAAAACGGTAACCATTTTTAAGCGGCGGGTTTGGTTGATGGGGCGGCTCCGCACACAAAAAGCCCGAAATATAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCagtag                                                                                                   <  6:291297/137‑1 (MQ=255)
                                  ttttAGCTATTGTTGCTTCACAAAAAGTTAAACATATTGATGGGCCGTGTTTGTTGAAAGTGGGGCACCCTCAGAGAAAAAGGCAGAAAAAGAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTacca                                                                                              <  2:240139/136‑1 (MQ=255)
                                  ttttAGCTATTGGTGATGGAAAAACTGGTATTCAATTTTCAGCGTAGTGGGTGGTTGAGGTGGCGGGTCCGCCTACACAACGGCCCACATACAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTacca                                                                                              <  2:240140/136‑1 (MQ=255)
                                  gTTTAGCGAATGCTGCTTCACAAAAACGTACACCATTTGAACCGCCCGGGTTGATGCAAGTGGCGGCCCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTacca                                                                                              <  6:175398/137‑1 (MQ=255)
                                  gTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTacca                                                                                              >  2:296559/1‑137 (MQ=255)
                                    ttAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTACCCCAGTAGTaccacc                                                                                            >  7:277926/1‑137 (MQ=255)
                                              ctgctgGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGTCGACCCGAAGGTCGCCTGGTTTCCCCCACTAGTACCACCACAGTGCTAc                                                                                  >  7:179413/1‑137 (MQ=255)
                                                gctgGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCg                                                                                >  8:248968/1‑137 (MQ=255)
                                                gctgGACAAAAAGGTAATCCAGTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCACCTGGTTTCCCCCAGTAGTACCCCCACAGTGCTACCg                                                                                >  6:364177/1‑137 (MQ=255)
                                                 ttgtaaaaaaaGTTAATCCTTTTTATGCGGCCTGGTTGCTTGAAGTGGCGGCGCCTCAGAGACAACGGCCTAAATAGAAAAGACGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCgg                                                                               <  5:192017/131‑1 (MQ=255)
                                                 ttgaaCAAAATGGTATTCATTTTGAGGGGTCCTGGTTGATTGATGTGGCGGCGCCTCATCCAAAACCGCCGACATATAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCgg                                                                               <  6:91659/133‑1 (MQ=255)
                                                 ctgGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCgg                                                                               <  7:248968/137‑1 (MQ=255)
                                                 ctgGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCgg                                                                               <  7:261067/137‑1 (MQ=255)
                                                      aaaaaaGTAAATCAATTTCAAGCGCCCTGGTTGCTTCAAGGGGCGGCGCCTCACACAAAACGCCCCAAAAACAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCcgc                                                                          <  3:127631/136‑1 (MQ=255)
                                                       aaaaaGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCcgcg                                                                         >  5:91659/1‑137 (MQ=255)
                                                           aGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTACTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGATTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCa                                                                     >  3:47495/1‑137 (MQ=255)
                                                            ggTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCaa                                                                    >  1:14010/1‑137 (MQ=255)
                                                              tAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTACCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTAAAgg                                                                  >  1:341949/1‑137 (MQ=255)
                                                                   aaTTTTAAGCGTCCGGTTTTCTTCACGTGGCGGCACCTCATAAAAAACGGCCTACATATAAAAAACGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGAtgtg                                                             <  8:277926/136‑1 (MQ=255)
                                                                    aTTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGGTCATGGATgtgg                                                            >  4:221064/1‑137 (MQ=255)
                                                                     tttcacgCGTCGTGGTGGCTTGACGGGGCGCCCCCTCACACAAAACCCCCCAAATACAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATgtggt                                                           <  5:211544/131‑1 (MQ=255)
                                                                     tttGACGGGGCCTGGTTGCGTGATGGGGCGGCGCCTCAGACACAACGGCCGACATAGAAAACCGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATgtggt                                                           <  6:113959/137‑1 (MQ=255)
                                                                     tttGAAGCGTCTTGTTTGATTGATGTGGCGGCCCCTCACACACAACGGCCTACTTACAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATgtggt                                                           <  7:167095/137‑1 (MQ=255)
                                                                     tttCAAGCGTCCTGGTTGCTTGAAGTGGCGGCGCCTCAGAGACAAAGCCCGACATACAAAAAACGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATgtggt                                                           <  5:279130/137‑1 (MQ=255)
                                                                        cacgCGTCCTGGTGGCTTCAAGGGGCGGCTCCTCACACACAAAGCCCCACATAGAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGca                                                        <  5:117241/134‑1 (MQ=255)
                                                                            cGTCCTGGGTGCTTCAAGGGGCGCCTCCTCAGACACAACGCCCGACATAGAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCAcacc                                                    <  6:142475/137‑1 (MQ=255)
                                                                                 tGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGaa                                               >  8:265430/1‑137 (MQ=255)
                                                                                  ggTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTCCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGaaa                                              >  8:232045/1‑137 (MQ=255)
                                                                                    ttGCTTTAAGGGGCGGCTCCCCCTACACAACGCCCCAAATATAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAact                                            <  5:302696/137‑1 (MQ=255)
                                                                                    gtGCTTGACGTCGCGCCTCCTCACAACCAAACCCCCACATATAAAAACCGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAact                                            <  7:354185/136‑1 (MQ=255)
                                                                                          gAAGTGGCGGCTCCTAATAGAAAAATTAAGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATTTGGTGCACACCACGAAACTACGGTc                                      >  3:14209/1‑137 (MQ=255)
                                                                                           aaGTGGCGGCTCCTAAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTACCCCAGTACTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACAACGAAACTACTGTcc                                     >  6:324187/1‑137 (MQ=255)
                                                                                                          agagACAAAGGCCGACATAGAAAAAAGGCGCCCCGAAGGTCGCCTGGTTTCCCACAGTAGTACCACCACAGTGCTACCGGGCCGCGTTGAAGCATGTTGGGCACACCCCCAAACAAATGTGCATGAACCCGGGGGtg                      >  3:330517/1‑136 (MQ=255)
                                                                                                          acacaCAACGCCCTACAAAGAAAAAACGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAACTACTGTCCTTGAACCCGGGGGTc                      <  5:192737/133‑1 (MQ=255)
                                                                                                           cagaCAAAGCCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAACTACTGTCCTTGAACCCGGGGGTcc                     <  8:92887/136‑1 (MQ=255)
                                                                                                              aCAAACGCCGAAATAGAAAAACGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAACTACTGTCCTTGAACCCGGGGGTCCAgg                  <  6:303694/137‑1 (MQ=255)
                                                                                                                              aaaaaaGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAACTACTGTCCTTGAACCCGGGGGTCCAGGCACAAGTGCCTGGGTg  <  7:160780/137‑1 (MQ=255)
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ACGGATTACACCGGCGAAACGAATTATGCAGAGCGTTTAGCGAATGCTGCTGGACAAAAAGGTAATCCATTTGAAGCGTCCTGGTTGCTTGAAGTGGCGGCTCCTCAGAGACAAAGGCCGACATAGAAAAAAGGCGACCCGAAGGTCGCCTGGTTTCCCCCAGTAGTACCACCACAGTGCTACCGGGCCGCGTTCAAGGATGTGGTGCACACCACGAAACTACTGTCCTTGAACCCGGGGGTCCAGGCACAAGTGCCTGGGTG  >  ACB143/3413441‑3413703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑