| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,390,258 | Δ2 bp | 100% | coding (352‑353/1666 nt) | ligH → | 10‑formyltetrahydrofolate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,390,258 | 0 | G | . | 93.8% | 128.5 / 3.5 | 32 | coding (352/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (7/23); total (8/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | ACB143 | 5,390,259 | 0 | T | . | 93.7% | 125.6 / 4.8 | 32 | coding (353/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (7/23); total (8/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCT > ACB143/5390133‑5390373 || aagaccacgaccaccgtgggcctggccgacggcctcaaccgcatcggcaagaagaccgtggcggcgctTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAt < 2:218559‑M2/68‑1 (MQ=255) aCGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACcttcact < 5:233302/136‑1 (MQ=255) aCGGCCTC‑ACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACcttcactt < 4:32501/136‑1 (MQ=255) cTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCAcc < 5:49870/136‑1 (MQ=255) gCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACt < 3:121752/136‑1 (MQ=255) cAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCCATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCCCTTCACCGGTGCCTTCCATGc > 8:49578/1‑136 (MQ=255) gTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCg < 3:296899/136‑1 (MQ=255) gcgAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGc < 3:18604/136‑1 (MQ=255) ccTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTcgcc < 8:215393/136‑1 (MQ=255) ctTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCg < 1:130656‑M2/134‑1 (MQ=255) ggCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATc < 1:277924/136‑1 (MQ=255) cccTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGAc < 5:49351/136‑1 (MQ=255) cccTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGAc < 1:83849/136‑1 (MQ=255) cccTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGAc < 5:111585/136‑1 (MQ=255) tGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAAc < 6:189780/136‑1 (MQ=255) tttCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAAcc < 7:208318/135‑1 (MQ=255) tCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACca > 1:83109/1‑136 (MQ=255) cGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTa < 6:77533/136‑1 (MQ=255) gTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAAc < 1:112280/58‑1 (MQ=255) gTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAAc > 2:112280/1‑58 (MQ=255) gTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGCCAACCACATCTAc > 1:125186/1‑136 (MQ=255) gTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTACATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTAc > 6:1085/1‑136 (MQ=255) aGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTggg > 6:71032/1‑136 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTgggg > 3:253941/1‑135 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGc < 8:7135/136‑1 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGc < 6:247952/136‑1 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGc < 4:286907/136‑1 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGc < 4:15818/136‑1 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGc < 2:223066/136‑1 (MQ=255) cgcggcCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACGTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGcaacaa < 3:237941/136‑1 (MQ=255) cgcggcCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGcaacaa < 2:38411/136‑1 (MQ=255) ggccggcGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCt > 4:263462/1‑136 (MQ=255) || CCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCT > ACB143/5390133‑5390373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |