Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,310,036:1 +C 100% coding (401/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,310,0361.C82.8% 185.0 / 32.3 58coding (401/1989 nt)aaeB_2p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base . (4/6);  new base C (28/20);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CCTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAG  >  ACB143/1309902‑1310169
                                                                                                                                       |                                                                                                                                      
ccTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCGACACCGCAGCGGTCATCGCCTTGCCGGCCATTGcccac                                                                                                                                       >  6:136289/1‑136 (MQ=255)
     ttCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCAcccccc                                                                                                                                 >  3:229869/1‑135 (MQ=255)
      tCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTTTGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGTCCATTGACCAcgcccc                                                                                                                                 <  6:67140/135‑2 (MQ=255)
      tCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCAcccccc                                                                                                                                 <  4:196932/135‑2 (MQ=255)
       cTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCAcccccct                                                                                                                                <  8:138118/135‑3 (MQ=255)
       cTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCGATGCCTTCGTGCTGGCCGGCGACACCTCAGCGAGCATCGCCTGGCCGGCCATTGACCAcaccccgg                                                                                                                               >  2:89413/1‑133 (MQ=255)
         gCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCa                                                                                                                              >  1:295794/1‑135 (MQ=255)
               cTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTc                                                                                                                       <  5:29092/136‑1 (MQ=255)
                 tGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGCCCACCCCCCTGCAAGTATTc                                                                                                                       >  4:17621/1‑135 (MQ=255)
                 tGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGCCCACCCCCCTGCAAGTATTc                                                                                                                       >  3:15126/1‑135 (MQ=255)
                 tGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCAGGCCATTGCCCACCCCCCTGCAAGTATTc                                                                                                                       >  6:175908/1‑135 (MQ=255)
                          ggctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                              <  1:298566/135‑1 (MQ=255)
                          ggctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                              <  5:71046/135‑1 (MQ=255)
                              ggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                         <  1:135796/136‑1 (MQ=255)
                               gcgcTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                         <  2:170439/135‑1 (MQ=255)
                               gcgcTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                         <  4:88097/135‑1 (MQ=255)
                               gcgcTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                         <  7:251533/134‑1 (MQ=255)
                                  cTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGc                                                                                                     >  6:105914/1‑136 (MQ=255)
                                     tgtACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTg                                                                                                   <  2:295794/134‑1 (MQ=255)
                                        aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTc                                                                                                                       >  3:65295/1‑112 (MQ=255)
                                        aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTc                                                                                                                       <  4:65295/112‑1 (MQ=255)
                                             aGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCgaga                                                                                           >  5:242206/1‑135 (MQ=255)
                                               cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                        <  3:125835/136‑1 (MQ=255)
                                               cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                        <  6:242206/136‑1 (MQ=255)
                                               cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                        >  5:273906/1‑136 (MQ=255)
                                                  ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGcc                                                                                     >  3:105842/1‑136 (MQ=255)
                                                  ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGcc                                                                                     >  3:188473/1‑136 (MQ=255)
                                                  ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGcc                                                                                     >  8:13983/1‑136 (MQ=255)
                                                    aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGAGCTGCCTgg                                                                                  >  8:77259/1‑136 (MQ=255)
                                                                aGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTCCCGAGATCTGCCTGGGTATGTTCtgt                                                                       >  6:153442/1‑136 (MQ=255)
                                                                aGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCtgt                                                                       >  8:26416/1‑136 (MQ=255)
                                                                aGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCtgt                                                                       <  8:100268/136‑1 (MQ=255)
                                                                     cTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCaa                                                                   >  3:173609/1‑135 (MQ=255)
                                                                       gggCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcag                                                                >  8:38258/1‑136 (MQ=255)
                                                                          ccTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca                                                             <  7:179719/136‑1 (MQ=255)
                                                                          ccTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca                                                             <  3:128506/136‑1 (MQ=255)
                                                                          ccTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca                                                             <  6:108160/136‑1 (MQ=255)
                                                                                ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGCCCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTg                                                       >  4:172091/1‑136 (MQ=255)
                                                                                ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                        <  1:107891/135‑1 (MQ=255)
                                                                                ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                        <  7:153606/135‑1 (MQ=255)
                                                                                cattCGTTATGGACTGCTAGAACGCAGCGATCATCGCCTTGCCGTCCATTGACCACCCACCTTATATTATTCGAGCAGGCCGTTGCTAGATGTAACGATATCTGCATGGGTATTTTATGTGCAAAAGCCATCAGt                                                        <  8:47554/133‑1 (MQ=255)
                                                                                 cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                        >  3:217853/1‑134 (MQ=255)
                                                                                 cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                        <  4:217853/134‑1 (MQ=255)
                                                                                 cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGc                                                      >  7:69240/1‑136 (MQ=255)
                                                                                 cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGc                                                      >  7:210264/1‑136 (MQ=255)
                                                                                 cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGcc                                                     >  1:194396/1‑136 (MQ=255)
                                                                                      tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc                                                 >  1:159033/1‑136 (MQ=255)
                                                                                      tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc                                                 >  4:55190/1‑136 (MQ=255)
                                                                                      tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                        <  3:190799/128‑1 (MQ=255)
                                                                                      tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                        >  4:190799/1‑128 (MQ=255)
                                                                                       gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTTTTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGct                                                >  5:219199/1‑136 (MQ=255)
                                                                                       gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGct                                                >  8:244380/1‑136 (MQ=255)
                                                                                       gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc                                                 >  4:285536/1‑135 (MQ=255)
                                                                                               gCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCa                                        <  3:276950/136‑1 (MQ=255)
                                                                                               gCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCa                                        <  5:98349/136‑1 (MQ=255)
                                                                                                   cacCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                              >  6:231364/1‑62 (MQ=255)
                                                                                                   cacCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                              <  5:231364/62‑1 (MQ=255)
                                                                                                   cacCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCg                                    >  2:275101/1‑136 (MQ=255)
                                                                                                       gCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTc                                <  4:173609/136‑1 (MQ=255)
                                                                                                        cAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCg                               >  2:96690/1‑136 (MQ=255)
                                                                                                           cGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGagc                            >  2:49478/1‑136 (MQ=255)
                                                                                                                           ggCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTgggcggg              >  3:199931/1‑134 (MQ=255)
                                                                                                                               aTTGACCACCCCCTGCAAGTAATCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCAGTGCAACAGCCAGCAGTGCCTTTCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGgcgc       <  4:137369/136‑1 (MQ=255)
                                                                                                                                ttGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGgcgc       <  2:187789/136‑1 (MQ=255)
                                                                                                                                    ccaccCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAg  <  3:13232/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCGCGGCAG  >  ACB143/1309902‑1310169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: