Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	ACB143	5,390,191	Δ10 bp	16.8%	coding (285‑294/1666 nt)	ligH →	10‑formyltetrahydrofolate synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB143	= 5390190	15 (0.340)	3 (0.070)	3/238	NT	16.8%	coding (284/1666 nt)	ligH	10‑formyltetrahydrofolate synthetase
	?	ACB143	5390201 =	15 (0.350)					coding (295/1666 nt)	ligH	10‑formyltetrahydrofolate synthetase

TTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5390055‑5390190 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCG  >  ACB143/5390201‑5390336                                                                                                                                                                                                                                                                                  TTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCT                                                                                                                                          <  1:198724/136‑1    AGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCgccg                                                                                                                                       >  3:264049/1‑132    AGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCcgcg                                                                                                                                       >  1:189055/1‑132    AGGGCAACCAGGACCGCAAGCTGAACCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAACGCAAGACCACGACCACCGTGGGCCTCGCCGACGGCCTCAACCGCATCGCCAAGAAGACCGTGGCGGCGCagcg                                                                                                                                       >  1:6190/1‑132                                                    CGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTAT                                                                                       <  1:146728/136‑1                                                     GCCGGCCGGCGAAGGCAAGACCACGACCCCCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATG                                                                                      <  8:218880/136‑1                                                                     AAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGAT                                                                      <  2:218559/136‑1                                                                                                                                       CTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCG  <  1:130656/136‑1                                                                                                                                                                                                                                                                                  TTCAGGGCAACCAGGACGGCAAGCTGATCCTCGTCACCGCGATCAACCCGACGCCGGCCGGCGAAGGCAAGACCACGACCACCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5390055‑5390190 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCG  >  ACB143/5390201‑5390336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.