| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 1,141,856 | C→G | 49.3% | D97H (GAC→CAC) | CJ019_01079 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,141,856 | 0 | C | G | 49.3% | 1.5 / 8.3 | 10 | D97H (GAC→CAC) | CJ019_01079 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base G (2/3); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-02 | |||||||||||
CAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGT > ACB143/1141734‑1141988 | cAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTAGGCCTGCAGgcgc > 5:287120/1‑136 (MQ=255) aCCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGCCCCCCGGTAGGTGGTGGGCCTGGAGGGGCCTGGGGAggg > 7:87118/1‑135 (MQ=255) cgcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTTGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGtt < 6:280765/135‑1 (MQ=255) ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTAACGACGGTGAATAGCCCGACCCGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAg > 7:238398/1‑136 (MQ=255) cgcCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCCCCCACCCGAACGTGGGGGGCCGGCACGCCCATGGGCCCGGTACCCACCGTGAAAAACCCCCCCCACACCCGCGCGGCGGTGGGCCCGGGGTTcccggga > 6:54554/1‑130 (MQ=255) gcgcgggcgctCGCCCCCCCCCTACTGGGGGTGGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCAt < 8:231388/136‑1 (MQ=255) gcgcgcgcCCACCCCCGTAGGGGGGGGGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTg < 1:69566/136‑1 (MQ=255) gcgcgcCCCGCCCACGGTAGGGGGGTGGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTg < 8:147958/136‑1 (MQ=255) cgggggtCGGCCTGCAGGCGCATGGGCAGGTAACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGGTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGGTGCTGCTGGTGCAt < 5:215259/130‑1 (MQ=255) ggtCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGGTGGTGCATGTGCGt > 6:35842/1‑136 (MQ=255) | CAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGT‑GGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGT > ACB143/1141734‑1141988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |