Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,302,662 G→A 7.0% R642C (CGC→TGC)  CJ019_01223 ← putative signaling protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,302,6620GA 7.0% 186.2 / 3.8 57R642C (CGC→TGC) CJ019_01223putative signaling protein
Reads supporting (aligned to +/- strand):  ref base G (21/32);  new base A (2/2);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

GAGGAACGCCTGCTGTTCGGCGGTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTGCGCTGGATATC  >  ACB143/1302528‑1302797
                                                                                                                                      |                                                                                                                                       
gAGGAACGCCTGCTGTTCGGCGGTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCgg                                                                                                                                        <  7:214219/136‑1 (MQ=255)
gAGGAACGCCTGCTGTTCGGCGGTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCgg                                                                                                                                        >  2:148694/1‑136 (MQ=255)
         ctgctgTTCGGCGGTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCtt                                                                                                                                >  7:63430/1‑135 (MQ=255)
                      gTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCg                                                                                                                  <  8:26158/136‑1 (MQ=255)
                      gTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCg                                                                                                                  <  5:131188/136‑1 (MQ=255)
                      gTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCg                                                                                                                  <  4:236576/136‑1 (MQ=255)
                      gTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCg                                                                                                                  <  4:124659/136‑1 (MQ=255)
                      gTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCg                                                                                                                  <  2:179872/136‑1 (MQ=255)
                            aCGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgc                                                                                                            <  6:244592/136‑1 (MQ=255)
                            aCGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgc                                                                                                            <  2:218470/136‑1 (MQ=255)
                            aCGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgc                                                                                                            <  8:158127/136‑1 (MQ=255)
                            aCGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgc                                                                                                            <  7:224267/136‑1 (MQ=255)
                              gCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgcgc                                                                                                          <  6:124719/136‑1 (MQ=255)
                                     cggACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGt                                                                                                   <  8:200504/134‑1 (MQ=255)
                                      tGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGt                                                                                                   >  1:13042/1‑135 (MQ=255)
                                        aCTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAg                                                                                                <  8:109121/136‑1 (MQ=255)
                                        aCTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAg                                                                                                <  2:69945/136‑1 (MQ=255)
                                            cccGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGcc                                                                                              <  1:210993/134‑1 (MQ=255)
                                               gCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGa                                                                                         >  6:164520/1‑136 (MQ=255)
                                               gCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGa                                                                                         >  6:31156/1‑136 (MQ=255)
                                                     gCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAg                                                                                     >  1:31214/1‑134 (MQ=255)
                                                                 ccATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCaa                                                                        >  7:88949/1‑135 (MQ=255)
                                                                      gCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgc                                                                                                            <  6:219296/94‑1 (MQ=255)
                                                                      gCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGgc                                                                                                            >  5:219296/1‑94 (MQ=255)
                                                                      gCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcat                                                                  >  1:283322/1‑136 (MQ=255)
                                                                      gCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcat                                                                  >  4:285546/1‑136 (MQ=255)
                                                                      gCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcat                                                                  >  5:179424/1‑136 (MQ=255)
                                                                      gCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcat                                                                  >  5:250329/1‑136 (MQ=255)
                                                                       cGATGATCGCCTGGACTATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcatc                                                                 <  4:15920/136‑1 (MQ=255)
                                                                        gatgatCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcatc                                                                 >  3:214481/1‑135 (MQ=255)
                                                                        gatgatCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAatcatc                                                                 >  2:93194/1‑135 (MQ=255)
                                                                          tgatCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCatcgat                                                              <  4:135575/136‑1 (MQ=255)
                                                                          tgatCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGGCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCatcgat                                                              <  8:210106/136‑1 (MQ=255)
                                                                                ccTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAg                                                        <  1:286501/136‑1 (MQ=255)
                                                                                 cTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGt                                                       <  1:84457/136‑1 (MQ=255)
                                                                                 cTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGAGCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGt                                                       <  6:222333/136‑1 (MQ=255)
                                                                                   ggACAATTTCGCGGTCTTTGTGGCTGCCCGGTTCATGCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGAAGAGTAGCCGGTACCTAAATCATCGTTCGAAAGTGc                                                     <  4:217557/136‑1 (MQ=255)
                                                                                   ggACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCATAATCATCGATCGAAAGTGc                                                     >  3:219013/1‑136 (MQ=255)
                                                                                            cccGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCAcccc                                               >  2:208986/1‑133 (MQ=255)
                                                                                                 tCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGccc                                       >  5:111346/1‑136 (MQ=255)
                                                                                                 tCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGccc                                       <  2:251925/136‑1 (MQ=255)
                                                                                                         gCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGt                               <  6:242869/136‑1 (MQ=255)
                                                                                                             cccGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGttt                             <  3:223692/134‑1 (MQ=255)
                                                                                                             cccGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGttt                             <  2:31214/134‑1 (MQ=255)
                                                                                                              ccGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAg                          >  1:161356/1‑136 (MQ=255)
                                                                                                                     aTCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCACGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTg                   <  4:239572/136‑1 (MQ=255)
                                                                                                                           cAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGt              <  5:257168/135‑1 (MQ=255)
                                                                                                                           cAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGt              <  1:248352/135‑1 (MQ=255)
                                                                                                                              gAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTgcgc          >  1:213926/1‑136 (MQ=255)
                                                                                                                              gAAGGTGCAGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGt                                                                                                   >  7:120293/1‑47 (MQ=255)
                                                                                                                              gAAGGTGCAGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGt                                                                                                   >  7:124204/1‑47 (MQ=255)
                                                                                                                              gAAGGTGCAGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGt                                                                                                   <  8:124204/47‑1 (MQ=255)
                                                                                                                              gAAGGTGCAGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGt                                                                                                   <  8:120293/47‑1 (MQ=255)
                                                                                                                               aaGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTGCGCt         <  3:239720/136‑1 (MQ=255)
                                                                                                                               aaGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTGCGCt         >  4:227287/1‑136 (MQ=255)
                                                                                                                               aaGGTGCGGTCGATCTTCAGCACATACAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGATAGTGCCCCCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGCCCGTGCGCt         >  2:58519/1‑136 (MQ=255)
                                                                                                                                 ggTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCCTCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTGCGCTgg       <  6:129545/136‑1 (MQ=255)
                                                                                                                                    gCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCAccc                                                <  6:58733/92‑1 (MQ=255)
                                                                                                                                    gCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCAccc                                                >  5:58733/1‑92 (MQ=255)
                                                                                                                                      ggTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCTGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTGCGCTGGATATc  >  6:72998/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GAGGAACGCCTGCTGTTCGGCGGTTTCCACGCCCTCGCTGACTACCCGCAGGTGCAGGGTATGCGCCATGGCGATGATCGCCTGGACAATTTCCCGGTCTTTCTGGCTGCCCGGTACATCCTGCAGGAAGGTGCGGTCGATCTTCAGCACATCCAGCGGCAGGCGCTTGAGGTAAGCCAGTGACGAGTAGCCGGTACCAAAATCATCGATCGAAAGTGCCACCCCCAGAGCCCTGACGCGTTTGAGCAGGCTGACCGTGCGCTGGATATC  >  ACB143/1302528‑1302797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑