Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,587,077 A→G 9.9% intergenic (‑219/+82) CJ019_01495 ← / ← kgtP_2 hypothetical protein/Alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,587,0770AG 9.9% 117.9 / 3.9 42intergenic (‑219/+82)CJ019_01495/kgtP_2hypothetical protein/Alpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base A (18/18);  new base G (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

ATTTGTTCGATGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTC  >  ACB143/1586959‑1587204
                                                                                                                      |                                                                                                                               
aTTTGTTCGATGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGaaa                                                                                                                <  4:190943/136‑1 (MQ=255)
aTTTGTTCGATGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGaa                                                                                                                 >  1:103486/1‑135 (MQ=255)
     ttCGATGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCt                                                                                                           >  1:284112/1‑136 (MQ=255)
             aTTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGa                                                                                                    >  6:179608/1‑135 (MQ=255)
                tGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGaaaa                                                                                                 <  4:91754/135‑1 (MQ=255)
                tGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGaaaa                                                                                                 <  1:26598/135‑1 (MQ=255)
                tGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGAGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGaaaa                                                                                                 <  7:40680/135‑1 (MQ=255)
                  ggCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAggg                                                                                              >  2:247595/1‑136 (MQ=255)
                       gTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCt                                                                                         >  8:199089/1‑136 (MQ=255)
                       gTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAAATAATAGGGAGTTAAGAAGGAAAAGTCTTAATAGAAAAGGGGAGCt                                                                                         >  3:167168/1‑136 (MQ=255)
                       gTATGTCGTGCTGCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCt                                                                                         <  8:116324/136‑1 (MQ=255)
                         aTGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTc                                                                                        >  8:108576/1‑135 (MQ=255)
                           gTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGGGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCAt                                                                                     >  8:2102/1‑136 (MQ=255)
                           gTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCAt                                                                                     >  8:51045/1‑136 (MQ=255)
                                    cAGGCCGCAAGGCTGGCTTGGGCGCGGTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAACGGGGCGCTCCATGCAATggg                                                                             <  7:271095/135‑1 (MQ=255)
                                     aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGgc                                                                           <  8:62323/136‑1 (MQ=255)
                                     aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGCAATTAATAGGGCGTTAAGAAGCAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGgc                                                                           <  5:154522/136‑1 (MQ=255)
                                     aGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTGCTGCCAGCCATTGCAGCTGGATAAGGTGGAATTAATAGGGCGTTAATAAGGAAAAGTCTGAATAGAAACGGGGCGCTCCATGCAATGGGgc                                                                           <  5:124050/136‑1 (MQ=255)
                                              ggcgggctTGGGAGCGTGCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATCGGGCGTTAAGAAGCAAAAGTCTGAATACAAAAGGGGCGCTCCATGCAATGGGGCGCCCCtttt                                                                  <  6:173312/136‑1 (MQ=255)
                                                   gctTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTAc                                                             <  4:251601/136‑1 (MQ=255)
                                                   gctTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTAc                                                             <  7:193078/136‑1 (MQ=255)
                                                      tGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTGCATGCAATGGGGGGCCCCTTTTAGTACTgc                                                          >  3:12686/1‑136 (MQ=255)
                                                      tGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgc                                                          >  1:110613/1‑136 (MQ=255)
                                                           gCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTGCATGCAATGGGGCGCCCCTTTTAGTACTgccaggc                                                     >  4:249810/1‑136 (MQ=255)
                                                             gTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccaggccc                                                   >  7:173589/1‑135 (MQ=255)
                                                             gTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTgccaggcca                                                   <  7:62345/136‑1 (MQ=255)
                                                                cgggggggCGCCAAGTTTCTGCCAGCCATTGCAGCTTTATATGGTGGAATTAATTGGGCGTTAAGAAGTAAAAGTCTTAATATAAACGGGGCGCTCCATGCATTGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCg                                                <  6:25962/131‑1 (MQ=255)
                                                                cgcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCg                                                >  2:52001/1‑136 (MQ=255)
                                                                 gcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATGGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGt                                               >  6:84867/1‑136 (MQ=255)
                                                                 gcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATGGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGt                                               >  2:249766/1‑136 (MQ=255)
                                                                 gcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGt                                               >  4:91335/1‑136 (MQ=255)
                                                                 gcgcgGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGt                                               >  4:184820/1‑136 (MQ=255)
                                                                        cgcCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAg                                        <  2:11543/136‑1 (MQ=255)
                                                                                     ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGa                           <  3:80938/136‑1 (MQ=255)
                                                                                     ccagccaTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGa                           <  7:199089/136‑1 (MQ=255)
                                                                                           aTTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGtgctg                     >  6:125028/1‑136 (MQ=255)
                                                                                               cAGCTTGATAAGGTGTAATTAATGGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                 <  1:249766/136‑1 (MQ=255)
                                                                                               cAGCTTGATAAGGTGGAATTAATGGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                 <  5:84867/136‑1 (MQ=255)
                                                                                               cAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTg                 <  3:91335/136‑1 (MQ=255)
                                                                                                aGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTgg                >  8:41288/1‑136 (MQ=255)
                                                                                                         aGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCa       <  3:250261/136‑1 (MQ=255)
                                                                                                              gAATTAATAGGGCGTTAAGAAGGAAAAGTCTTAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTc  <  3:194517/136‑1 (MQ=255)
                                                                                                                      |                                                                                                                               
ATTTGTTCGATGTATTTGGGCGCGTATGTCGTGCTTCAGGCCGCAAGGCTGGCTTGGGAGCGTTCGCGCGGGCGCCAACGTTCTGCCAGCCATTGCAGCTTGATAAGGTGGAATTAATAGGGCGTTAAGAAGGAAAAGTCTGAATAGAAAAGGGGCGCTCCATGCAATGGGGCGCCCCTTTTAGTACTGCCAGGCCAGCGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTC  >  ACB143/1586959‑1587204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑