Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,171,262 C→A 9.3% F147L (TTC→TTA CJ019_02060 → IS1182 family transposase ISPpu16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2620CA 9.3% 280.8 / 5.2 128F147L (TTC→TTACJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base C (60/54);  new base A (2/12);  total (62/66)
Fisher's exact test for biased strand distribution p-value = 9.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG  >  ACB143/2171128‑2171391
                                                                                                                                      |                                                                                                                                  
gATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCa                                                                                                                                   <  8:156687/136‑1 (MQ=255)
gATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCa                                                                                                                                   <  8:153257/136‑1 (MQ=255)
  tttCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCaaa                                                                                                                                 <  7:147840/136‑1 (MQ=255)
  tttCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCaaa                                                                                                                                 <  6:52077/136‑1 (MQ=255)
      aaaaCCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAg                                                                                                                            <  5:126276/136‑1 (MQ=255)
             tCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACaa                                                                                                                     >  8:238579/1‑136 (MQ=255)
              cGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACaa                                                                                                                     >  2:5723/1‑135 (MQ=255)
               gCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATc                                                                                                                   >  3:183407/1‑136 (MQ=255)
                ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATcg                                                                                                                  >  4:281511/1‑136 (MQ=255)
                ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATcg                                                                                                                  >  7:18508/1‑136 (MQ=255)
                ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTCAACCTTTTCTCTGAGTAGATCATTGCAATCGACGGCCGCAAGTTAAAACCAGTGAACAATcg                                                                                                                  >  2:109428/1‑136 (MQ=255)
                acGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTAAACATTTTCACTGATTCGATAATTGCCATCGACTGCAGCAAGTTCAAAGCAATGAACAAtac                                                                                                                  >  6:4365/2‑134 (MQ=255)
                    cTTCCGAAAGCACAACGTAAAACCCATTCGTAGCTGCTGCCTTCAGTTCGTTATCCTCTGCCTAAACCACAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAACGCAGTCAACAAGCGCGa                                                                                                               <  8:274384/135‑1 (MQ=255)
                         ccAAGGACACCGGAAAAGCCATTCGTAGCGTCTGCCGGCTGTTCGTTATCCTCTGCCTTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGAGGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTa                                                                                                          <  6:275806/134‑1 (MQ=255)
                             ggACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCTATCATTGCCATCGACGGCAGAAAGTTAAAAGCAGTGAACAATCGCGACCGTAACTTc                                                                                                     <  7:217998/136‑1 (MQ=255)
                             ggACAACGGTAAAGCAATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTc                                                                                                     <  7:213908/136‑1 (MQ=255)
                                    ttaaaaGCCATTCTTAGCTTCTGCAGTTTGTGCGTTATCTTAGCCCCAAACCAAAACCTTTTATCTTAGCCTATTATTGCCTTCGACGGCTGAATTTCAAATGCTGTGAACAATCGCGACCGTAACTTCACCCAgg                                                                                              <  2:32731/133‑1 (MQ=255)
                                    gtaaaaGCATTTCGTAGCGTCTGCCTTCTGTTCGTTATCCTCTGCCTAAACCTAAACTTTTTCTGTGCGTCTATCATTGCCATCGAGGGAAGCAAGTTCAACGCAGTTAACAAGCGCGACCGTAACTTCACCCAgg                                                                                              <  8:68002/133‑1 (MQ=255)
                                    gtaaaaCCCATTCTTCGCGTCTGCCGTCATTTCGTGATCCTCTGCCGAAACCTAAACCTTTTCTCTGAGTCTATCATTCCAATCGACGGCAGAATTTTCAAAGCTGTAAAAAACCGCGACCGTAACTTCACCCAgg                                                                                              <  2:18784/133‑1 (MQ=255)
                                         cgCCATTCGTCGCGTCGCCCGGCTGTTCGTGATCCTCTGCCGAACCCACAACGTTTTCTCTGAGTCGATCTTTCCCCGCCAGGGCAGCAATTACAAAGCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAg                                                                                         <  6:253444/135‑1 (MQ=255)
                                           ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                       >  1:245257/1‑136 (MQ=255)
                                            cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                       >  3:291990/1‑135 (MQ=255)
                                                 gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCCACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGACAAGGTGAAGGc                                                                                 >  5:120436/1‑136 (MQ=255)
                                                 gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGCACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc                                                                                 >  2:111223/1‑136 (MQ=255)
                                                 gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  8:197299/1‑136 (MQ=255)
                                                 gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  3:265994/1‑136 (MQ=255)
                                                 gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  3:204473/1‑136 (MQ=255)
                                                 gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  7:30053/1‑136 (MQ=255)
                                                   aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGCAGGccc                                                                               >  8:246045/1‑136 (MQ=255)
                                                   aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTATCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               >  1:20438/1‑136 (MQ=255)
                                                    gggTCGGCCTTCAGTTCGTCCACCTCTGCCGAAACCACAACCTTTTCGCTCTGCCCACAATGGCCACCGCCGCCAGCAAGTAAAACGCAGTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               <  4:9552/133‑1 (MQ=255)
                                                    gCGTCTGACGTCCTTTCGTGATCGAGTGCCGTACCCTCAACCTTTTCTCTGTGCCTATCATTGCCATCTACGGCAGAAAGTTCAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               <  6:137429/135‑1 (MQ=255)
                                                        cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                          >  6:280020/1‑136 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTTAAATCGACGGCAGCAAGTTCAAACCAGTGAACAAACGCGACCGTAACTTCACCCACGCCAAGGTTAAGGCCCGCATgaag                                                                       >  1:102292/1‑135 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  2:300399/1‑135 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCACGGTGAAGGCCCGCATgcag                                                                       >  6:163916/1‑135 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCACGTTCAAAGCAGTGAACAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgccg                                                                       >  8:67110/1‑133 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  4:168280/1‑135 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  5:283348/1‑135 (MQ=255)
                                                            cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  8:125555/1‑135 (MQ=255)
                                                              tCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca                                                                     >  5:271319/1‑135 (MQ=255)
                                                                 gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc                                                                 >  4:13468/1‑136 (MQ=255)
                                                                  tgcGTGAGCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGTCGGCAGCAAGTTCAACGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                <  2:191056/134‑1 (MQ=255)
                                                                    ggTGAGCCTCTGCCGAACCCTAAACTTTTTTTCTGTGTGTATAATTCCCATCGAGGGCAGAAATTAAAACGAAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                               <  5:88086/134‑1 (MQ=255)
                                                                    cGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAACCCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                               <  7:26899/135‑1 (MQ=255)
                                                                         tCCGCTGCCTTAACATCAAATTTTTCTGTGAGTGGATCATTCCCATCTACGGCAGAATTTACAACCCTTTTAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                          <  4:142153/135‑1 (MQ=255)
                                                                           ctctGCCTTACACACACCCTTTTCTCTGAGTCTATCATTGCCATCGCGGGCAGCAAGATAACACACGCCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca                                                       <  6:25316/136‑1 (MQ=255)
                                                                           ctctGCCTTAACCTCAACCTTTTCTCTTAGTCGTTCTTTCCCATCTCCGGCTGCATGTTCAATCCAGTCAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca                                                       <  7:157472/136‑1 (MQ=255)
                                                                            tctGCCGTAACCTCATCATTTTCTGTGTGTCTATCATTGCCAGCGAGGGCAGCAATTTCAACCCAGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAt                                                      <  1:79264/136‑1 (MQ=255)
                                                                             ctGCCTTAACCACAACTTTTAGTCTGAGACAATAATTCCCATCGACGGCAGAATTTTCAAACCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     <  1:152537/136‑1 (MQ=255)
                                                                             ctGCCGTAACCTCAACCTTTTCTCTGAGTCGATAATTGCCATCGAGGGCAGAAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     <  2:25/136‑1 (MQ=255)
                                                                               gcctaaaCCACAACGTTTTCTCTGAGTCCATCATTGCCATCGAGGGCAGAAAGTCAAACCCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:34387/131‑1 (MQ=255)
                                                                               gCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     >  8:132514/1‑134 (MQ=255)
                                                                               gCCGTAACCTCAACATTTTCTCTCAGTGTATCATGGACATCTAGGGCAGCATTTTCAAAGCATTAAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:251510/136‑1 (MQ=255)
                                                                               cccttAACCTCAACCTTTTCTCTTAGTCGATCATGCCCATCCACGGCAGCAATTCAAACCCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:66802/132‑1 (MQ=255)
                                                                                ccttAACCTCAACCTTTTCTCTGAGTCGATCATTCCAATCGAGGGCAGCAAGTTAAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:31127/132‑1 (MQ=255)
                                                                                ccttAACATCAACCTTTTCTCTGAGTCTATCATTCCAATCGACGGCACAAAGTTCAAAGAATTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:233041/132‑1 (MQ=255)
                                                                                ccgaacccTCAACCTTTCATCGGAGTGCTTAATTCCCATCTACGGCAGAATTTTAAACGCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:254011/129‑1 (MQ=255)
                                                                                ccGTAACCTCACCCTTTGCTCTGAGTCTATCATTCCCTGCGCGGGCAGCAATTGAAAAGCAGTCAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  1:79545/135‑1 (MQ=255)
                                                                                ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAACGCAGTCAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  8:177882/135‑1 (MQ=255)
                                                                                ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:212087/135‑1 (MQ=255)
                                                                                ccGTAACCTAAACCTTTTCTCTGAGCCTATAATTGCCATCGACGGCAGAAATTTAAAAGCAGTAAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  5:197819/135‑1 (MQ=255)
                                                                                ccGAAACCTCAACTTTTTCTCTGCGTCTATATTTGCCAGCGAGGGCAGCATTTTCAAACCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  5:233043/135‑1 (MQ=255)
                                                                                ccGAAACCTCAACCTTTTCTCTGCGGCTATCATGGCAATCGACGGAAGCAAGTTCAACGCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  2:185588/135‑1 (MQ=255)
                                                                                ccGAAACCTAAACCTTTTTTCTTTGTCGATCTTTCCCATGCCCGGCAGCAAGTTCAAACCTTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:105626/135‑1 (MQ=255)
                                                                                 cGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc                                                 >  8:215153/1‑136 (MQ=255)
                                                                                 cGTAACCTCAACCTTTTATCTGAGTCGATCATTGCCATCGCCGGCACCAAGTTCAAAGCATTGAAAAATCGCGACCGTAACATAACACAGGCCAATGTGAAGTCCCGCATTCATCAAATCGAACAGAGCAGTGAgc                                                 >  2:241525/1‑136 (MQ=255)
                                                                                      ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                            >  4:232624/1‑136 (MQ=255)
                                                                                      ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGCTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                            >  6:182006/1‑136 (MQ=255)
                                                                                       cTCAACCTTTTCTCTGAGTCGATAATTGCCATCGACGGCAGCAAGTTCAAAGCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  2:15689/136‑1 (MQ=255)
                                                                                       cTCAACATTTTCTCTTAGTCGATCTTTGCCAGCGACGGCAGCAAGTTCAAAGCTGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  3:227447/136‑1 (MQ=255)
                                                                                        tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCCCCACCGAAACTTCACCCCGGCCAAGGAGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCt                                          >  6:63848/1‑136 (MQ=255)
                                                                                            ctttttATCTGTGCCTATCATTCCCATCTCCGGCACCAAGTCAAACGCTCTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa                                      <  3:160107/134‑1 (MQ=255)
                                                                                            ccTTTTCTCTGAGTAGATCTTTCCCATCGACGGCAGCAAGTTCAAAGCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa                                      <  4:29448/136‑1 (MQ=255)
                                                                                             cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg                                     >  2:103265/1‑136 (MQ=255)
                                                                                             cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  5:188644/1‑136 (MQ=255)
                                                                                             cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  3:118843/1‑136 (MQ=255)
                                                                                                   tctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                >  7:159993/1‑135 (MQ=255)
                                                                                                    ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAATGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCTATGGa                              >  2:260847/1‑136 (MQ=255)
                                                                                                    ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                              >  6:187775/1‑136 (MQ=255)
                                                                                                          cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                         >  1:243996/1‑135 (MQ=255)
                                                                                                          cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                         >  5:258647/1‑135 (MQ=255)
                                                                                                           gATCATTGCCATCGAGGGCAGCAAGTTCAAAGCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                        <  7:29509/135‑1 (MQ=255)
                                                                                                             tctttGCCATCGACGGCAGCAAGTTCAAAGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                        <  6:49490/130‑1 (MQ=255)
                                                                                                               aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  2:131794/1‑136 (MQ=255)
                                                                                                               aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  5:96051/1‑136 (MQ=255)
                                                                                                               aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  8:177987/1‑136 (MQ=255)
                                                                                                               aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  1:175759/1‑136 (MQ=255)
                                                                                                                ttcccATCCACGCCAGCAAGTTCAACGCCGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  1:22823/133‑1 (MQ=255)
                                                                                                                atGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  >  1:102720/2‑136 (MQ=255)
                                                                                                                atGCCAGCCACGTCAGCACGTACAAACCAGTCACCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  4:28992/135‑1 (MQ=255)
                                                                                                                 gcccATGGACGTCAGCAATTACACCCCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  5:87928/133‑1 (MQ=255)
                                                                                                                    cATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc               >  7:284219/1‑135 (MQ=255)
                                                                                                                      tCGACGGCACCAAGTACAACCCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAAt            <  6:270676/136‑1 (MQ=255)
                                                                                                                          cGGCCGCAAGTTCAAAGCCGGCACCAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  6:282085/136‑1 (MQ=255)
                                                                                                                          cGGCATCAAGTTCCACTCTGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  5:199630/136‑1 (MQ=255)
                                                                                                                          cGGCAGAAAGTTCAACGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  7:79980/136‑1 (MQ=255)
                                                                                                                              agcaAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  4:4662/1‑136 (MQ=255)
                                                                                                                              agcaAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  3:80225/1‑136 (MQ=255)
                                                                                                                                aaaGTTCAAAGCAGATAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg  <  2:259126/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG  >  ACB143/2171128‑2171391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: