Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 2,171,262 | C→A | 9.3% | F147L (TTC→TTA) | CJ019_02060 → | IS1182 family transposase ISPpu16 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 2,171,262 | 0 | C | A | 9.3% | 280.8 / 5.2 | 128 | F147L (TTC→TTA) | CJ019_02060 | IS1182 family transposase ISPpu16 |
Reads supporting (aligned to +/- strand): ref base C (60/54); new base A (2/12); total (62/66) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.12e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 128 total aligned reads displayed.
GATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG > ACB143/2171128‑2171391 | gATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCa < 8:156687/136‑1 (MQ=255) gATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCa < 8:153257/136‑1 (MQ=255) tttCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCaaa < 7:147840/136‑1 (MQ=255) tttCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCaaa < 6:52077/136‑1 (MQ=255) aaaaCCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAg < 5:126276/136‑1 (MQ=255) tCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACaa > 8:238579/1‑136 (MQ=255) cGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACaa > 2:5723/1‑135 (MQ=255) gCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATc > 3:183407/1‑136 (MQ=255) ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATcg > 4:281511/1‑136 (MQ=255) ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATcg > 7:18508/1‑136 (MQ=255) ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTCAACCTTTTCTCTGAGTAGATCATTGCAATCGACGGCCGCAAGTTAAAA‑CCAGTGAACAATcg > 2:109428/1‑136 (MQ=255) acGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTAAACATTTTCACTGATTCGATAATTGCCATCGACTGCAGCAAGTTCAAA‑GCAATGAACAAtac > 6:4365/2‑134 (MQ=255) cTTCCGAAAGCACAACGTAAAACCCATTCGTAGCTGCTGCCTTCAGTTCGTTATCCTCTGCCTAAACCACAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAC‑GCAGTCAACAAGCGCGa < 8:274384/135‑1 (MQ=255) ccAAGGACACCGGAAAAGCCATTCGTAGCGTCTGCCGGCTGTTCGTTATCCTCTGCCTTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGAGGGCAGCAAGTTCAAA‑GCAGTTAACAATCGCGACCGTa < 6:275806/134‑1 (MQ=255) ggACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCTATCATTGCCATCGACGGCAGAAAGTTAAAA‑GCAGTGAACAATCGCGACCGTAACTTc < 7:217998/136‑1 (MQ=255) ggACAACGGTAAAGCAATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTTAACAATCGCGACCGTAACTTc < 7:213908/136‑1 (MQ=255) ttaaaaGCCATTCTTAGCTTCTGCAGTTTGTGCGTTATCTTAGCCCCAAACCAAAACCTTTTATCTTAGCCTATTATTGCCTTCGACGGCTG‑AATTTCAAATGCTGTGAACAATCGCGACCGTAACTTCACCCAgg < 2:32731/133‑1 (MQ=255) gtaaaaGCATTTCGTAGCGTCTGCCTTCTGTTCGTTATCCTCTGCCTAAACCTAAACTTTTTCTGTGCGTCTATCATTGCCATCGAGGGAAGCAAGTTCAAC‑GCAGTTAACAAGCGCGACCGTAACTTCACCCAgg < 8:68002/133‑1 (MQ=255) gtaaaaCCCATTCTTCGCGTCTGCCGTCATTTCGTGATCCTCTGCCGAAACCTAAACCTTTTCTCTGAGTCTATCATTCCAATCGACGGCAGAATTTTCAAA‑GCTGTAAAAAACCGCGACCGTAACTTCACCCAgg < 2:18784/133‑1 (MQ=255) cgCCATTCGTCGCGTCGCCCGGCTGTTCGTGATCCTCTGCCGAACCCACAACGTTTTCTCTGAGTCGATCTTTCCCCGCCAGGGCAGCAATTACAAA‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAg < 6:253444/135‑1 (MQ=255) ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt > 1:245257/1‑136 (MQ=255) cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt > 3:291990/1‑135 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCCACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGACAAGGTGAAGGc > 5:120436/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGCACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc > 2:111223/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 8:197299/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 3:265994/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 3:204473/1‑136 (MQ=255) gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc > 7:30053/1‑136 (MQ=255) aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGCAGGccc > 8:246045/1‑136 (MQ=255) aGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTATCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAAAAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGccc > 1:20438/1‑136 (MQ=255) gggTCGGCCTTCAGTTCGTCCACCTCTGCCGAAACCACAACCTTTTCGCTCTGCCCACAATGGCCACCGCCGCCAGCAAGTAAAAC‑GCAGTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc < 4:9552/133‑1 (MQ=255) gCGTCTGACGTCCTTTCGTGATCGAGTGCCGTACCCTCAACCTTTTCTCTGTGCCTATCATTGCCATCTACGGCAGAAAGTTCAAA‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc < 6:137429/135‑1 (MQ=255) cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg > 6:280020/1‑136 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTTAAATCGACGGCAGCAAGTTCAAA‑CCAGTGAACAAACGCGACCGTAACTTCACCCACGCCAAGGTTAAGGCCCGCATgaag > 1:102292/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCATgcag > 2:300399/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGCCGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCACGGTGAAGGCCCGCATgcag > 6:163916/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCACGTTCAAA‑GCAGTGAACAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgccg > 8:67110/1‑133 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag > 4:168280/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag > 5:283348/1‑135 (MQ=255) cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGCCCGCATgcag > 8:125555/1‑135 (MQ=255) tCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca > 5:271319/1‑135 (MQ=255) gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc > 4:13468/1‑136 (MQ=255) tgcGTGAGCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGTCGGCAGCAAGTTCAAC‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg < 2:191056/134‑1 (MQ=255) ggTGAGCCTCTGCCGAACCCTAAACTTTTTTTCTGTGTGTATAATTCCCATCGAGGGCAGAAATTAAAAC‑GAAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga < 5:88086/134‑1 (MQ=255) cGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAC‑CCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga < 7:26899/135‑1 (MQ=255) tCCGCTGCCTTAACATCAAATTTTTCTGTGAGTGGATCATTCCCATCTACGGCAGAATTTACAAC‑CCTTTTAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga < 4:142153/135‑1 (MQ=255) ctctGCCTTACACACACCCTTTTCTCTGAGTCTATCATTGCCATCGCGGGCAGCAAGATAACA‑CACGCCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca < 6:25316/136‑1 (MQ=255) ctctGCCTTAACCTCAACCTTTTCTCTTAGTCGTTCTTTCCCATCTCCGGCTGCATGTTCAAT‑CCAGTCAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca < 7:157472/136‑1 (MQ=255) tctGCCGTAACCTCATCATTTTCTGTGTGTCTATCATTGCCAGCGAGGGCAGCAATTTCAAC‑CCAGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAt < 1:79264/136‑1 (MQ=255) ctGCCTTAACCACAACTTTTAGTCTGAGACAATAATTCCCATCGACGGCAGAATTTTCAAA‑CCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt < 1:152537/136‑1 (MQ=255) ctGCCGTAACCTCAACCTTTTCTCTGAGTCGATAATTGCCATCGAGGGCAGAAAGTTCAAA‑GCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt < 2:25/136‑1 (MQ=255) gcctaaaCCACAACGTTTTCTCTGAGTCCATCATTGCCATCGAGGGCAGAAAGTCAAAC‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:34387/131‑1 (MQ=255) gCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt > 8:132514/1‑134 (MQ=255) gCCGTAACCTCAACATTTTCTCTCAGTGTATCATGGACATCTAGGGCAGCATTTTCAAA‑GCATTAAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:251510/136‑1 (MQ=255) cccttAACCTCAACCTTTTCTCTTAGTCGATCATGCCCATCCACGGCAGCAA‑TTCAAACCCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 3:66802/132‑1 (MQ=255) ccttAACCTCAACCTTTTCTCTGAGTCGATCATTCCAATCGAGGGCAGCAAGTTAAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 3:31127/132‑1 (MQ=255) ccttAACATCAACCTTTTCTCTGAGTCTATCATTCCAATCGACGGCACAAAGTTCAAA‑GAATTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 3:233041/132‑1 (MQ=255) ccgaacccTCAACCTTTCATCGGAGTGCTTAATTCCCATCTACGGCAGAATTTTAAAC‑GCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:254011/129‑1 (MQ=255) ccGTAACCTCACCCTTTGCTCTGAGTCTATCATTCCCTGCGCGGGCAGCAATTGAAAA‑GCAGTCAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 1:79545/135‑1 (MQ=255) ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAC‑GCAGTCAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 8:177882/135‑1 (MQ=255) ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 7:212087/135‑1 (MQ=255) ccGTAACCTAAACCTTTTCTCTGAGCCTATAATTGCCATCGACGGCAGAAATTTAAAA‑GCAGTAAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 5:197819/135‑1 (MQ=255) ccGAAACCTCAACTTTTTCTCTGCGTCTATATTTGCCAGCGAGGGCAGCATTTTCAAA‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 5:233043/135‑1 (MQ=255) ccGAAACCTCAACCTTTTCTCTGCGGCTATCATGGCAATCGACGGAAGCAAGTTCAAC‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 2:185588/135‑1 (MQ=255) ccGAAACCTAAACCTTTTTTCTTTGTCGATCTTTCCCATGCCCGGCAGCAAGTTCAAA‑CCTTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa < 4:105626/135‑1 (MQ=255) cGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc > 8:215153/1‑136 (MQ=255) cGTAACCTCAACCTTTTATCTGAGTCGATCATTGCCATCGCCGGCACCAAGTTCAAA‑GCATTGAAAAATCGCGACCGTAACATAACACAGGCCAATGTGAAGTCCCGCATTCATCAAATCGAACAGAGCAGTGAgc > 2:241525/1‑136 (MQ=255) ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc > 4:232624/1‑136 (MQ=255) ccTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGCTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc > 6:182006/1‑136 (MQ=255) cTCAACCTTTTCTCTGAGTCGATAATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc < 2:15689/136‑1 (MQ=255) cTCAACATTTTCTCTTAGTCGATCTTTGCCAGCGACGGCAGCAAGTTCAAA‑GCTGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc < 3:227447/136‑1 (MQ=255) tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCCCCACCGAAACTTCACCCCGGCCAAGGAGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCt > 6:63848/1‑136 (MQ=255) ctttttATCTGTGCCTATCATTCCCATCTCCGGCACCAAGTCAAAC‑GCTCTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa < 3:160107/134‑1 (MQ=255) ccTTTTCTCTGAGTAGATCTTTCCCATCGACGGCAGCAAGTTCAAA‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa < 4:29448/136‑1 (MQ=255) cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg > 2:103265/1‑136 (MQ=255) cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg > 5:188644/1‑136 (MQ=255) cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg > 3:118843/1‑136 (MQ=255) tctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg > 7:159993/1‑135 (MQ=255) ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAATGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCTATGGa > 2:260847/1‑136 (MQ=255) ctGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa > 6:187775/1‑136 (MQ=255) cGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg > 1:243996/1‑135 (MQ=255) cGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg > 5:258647/1‑135 (MQ=255) gATCATTGCCATCGAGGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc < 7:29509/135‑1 (MQ=255) tctttGCCATCGACGGCAGCAAGTTCAAA‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc < 6:49490/130‑1 (MQ=255) aTTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 2:131794/1‑136 (MQ=255) aTTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 5:96051/1‑136 (MQ=255) aTTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 8:177987/1‑136 (MQ=255) aTTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc > 1:175759/1‑136 (MQ=255) ttcccATCCACGCCAGCAAGTTCAAC‑GCCGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg < 1:22823/133‑1 (MQ=255) atGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg > 1:102720/2‑136 (MQ=255) atGCCAGCCACGTCAGCACGTACAAA‑CCAGTCACCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg < 4:28992/135‑1 (MQ=255) gcccATGGACGTCAGCAATTACACC‑CCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg < 5:87928/133‑1 (MQ=255) cATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc > 7:284219/1‑135 (MQ=255) tCGACGGCACCAAGTACAAC‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAAt < 6:270676/136‑1 (MQ=255) cGGCCGCAAGTTCAAA‑GCCGGCACCAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 6:282085/136‑1 (MQ=255) cGGCATCAAGTTCCAC‑TCTGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 5:199630/136‑1 (MQ=255) cGGCAGAAAGTTCAAC‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg < 7:79980/136‑1 (MQ=255) agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg > 4:4662/1‑136 (MQ=255) agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg > 3:80225/1‑136 (MQ=255) aaaGTTCAAA‑GCAGATAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg < 2:259126/135‑1 (MQ=255) | GATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG > ACB143/2171128‑2171391 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |