Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,353,557 G→A 8.4% L213L (CTG→TTG)  CJ019_02227 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,353,5570GA 8.4% 142.4 / 2.7 49L213L (CTG→TTG) CJ019_02227hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GGTGCTCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTCGGTCGA  >  ACB143/2353426‑2353690
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ggTGCTCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCg                                                                                                                                   >  5:111322/1‑136 (MQ=255)
ggTGCTCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCAATGTGAGGCAGGCCg                                                                                                                                   >  5:1512/1‑136 (MQ=255)
     tCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCg                                                                                                                              >  4:236400/1‑136 (MQ=255)
     tCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCg                                                                                                                              >  2:82669/1‑136 (MQ=255)
     tCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCg                                                                                                                              >  6:231244/1‑136 (MQ=255)
      cAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCgg                                                                                                                             >  8:269686/1‑136 (MQ=255)
          cgggggcgggggACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTgg                                                                                                                         >  4:163959/1‑136 (MQ=255)
           gggggcggcggACGGCGTGTGGGTCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCGGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTggg                                                                                                                        <  5:248334/136‑1 (MQ=255)
               gcgggggACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGc                                                                                                                    >  6:113351/1‑136 (MQ=255)
                ggggggACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGcc                                                                                                                   <  4:254946/135‑1 (MQ=255)
                      aCGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGAc                                                                                                               >  5:155718/1‑134 (MQ=255)
                         gCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGc                                                                                                          <  4:246349/136‑1 (MQ=255)
                              tgcgCCTTCTTCCATATATCGACGGATGACTACCCACTCATCACGCAGTTCGAAGGCGCCGTGGCCTTGTTTGCCCAGCAGGGCCATTTCAATGCGCGGCAGGCCGGTGCGGGGGGGGTGCCAGACCAGGCCGAg                                                                                                      <  8:77941/132‑1 (MQ=14)
                              tgGGCCTTCTTCCATATAACCACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGTCCATTTCCTAGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAg                                                                                                      <  4:285296/135‑1 (MQ=255)
                                 gCCTTCATCCATATTACGACGGATGAATTCCCACTGATCACTCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGGAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCcgc                                                                                                  <  6:1512/136‑1 (MQ=17)
                                   cttcttCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGt                                                                                                <  1:264813/136‑1 (MQ=255)
                                   cttcttCCATATAACGACGGATGAAATCCCACTCCTCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGTGGTGCCAGACCAGGCCGAGCCGCGt                                                                                                <  1:245985/136‑1 (MQ=255)
                                   cttcatCCATAAAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGGCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGt                                                                                                <  7:233734/136‑1 (MQ=255)
                                       ttcCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGccc                                                                                            <  3:84786/136‑1 (MQ=255)
                                         ccATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGccc                                                                                            <  8:234065/134‑1 (MQ=255)
                                         ccATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGATAGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCTGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGccc                                                                                            <  1:211490/134‑1 (MQ=255)
                                               aaCGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCtgag                                                                                    >  1:199557/1‑136 (MQ=255)
                                                    cgGATGAATTCCCCCTCATCACGCAGTTCGCTGCCACCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAAGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGcc                                                                                                    <  5:29064/115‑1 (MQ=255)
                                                    cgGATGAATTCCCACTCATCACGCAGTTCGCTGCCCCCTAGGCCTTGTTTGCACAGCAGGGCCATTACCATGTGAGGCACGCCGCAGCCGCTCGGGTGCAAGACCAGGCCGACCCGCGTGCCCACGCTgagtgagt                                                                               >  4:81916/1‑136 (MQ=18)
                                                    cgGATGAATTCCCACTCATCACGCAGTTCGCTGCCACCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAAGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGcc                                                                                                    >  6:29064/1‑115 (MQ=255)
                                                    cgGATGAATTCCCACTCATCACGCAGTTCGCTGCCACCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAAGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGcc                                                                                                    >  6:25508/1‑115 (MQ=255)
                                                    cgGATGAATTCCCACTCATCACGCAGTTCGCTGCCACCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAAGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGcc                                                                                                    <  5:25508/115‑1 (MQ=255)
                                                      gATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCa                                                                             >  2:277384/1‑136 (MQ=255)
                                                       aTGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCAt                                                                            >  1:188035/1‑136 (MQ=255)
                                                         gAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCAtct                                                                          >  7:230976/1‑136 (MQ=255)
                                                                     atcaCGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATg                                                              <  2:296532/136‑1 (MQ=255)
                                                                        aCGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGcg                                                           >  3:174361/1‑135 (MQ=255)
                                                                          gCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCtgt                                                         <  2:1635/136‑1 (MQ=255)
                                                                                cGCTGCCACCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTcc                                                   >  1:35858/1‑136 (MQ=255)
                                                                                                     tGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGt                              >  1:284548/1‑136 (MQ=255)
                                                                                                          agcagGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGa                         >  3:185003/1‑136 (MQ=255)
                                                                                                          agcagGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGa                         >  8:179795/1‑136 (MQ=255)
                                                                                                          agcagGGACATTTCCATGTGAGGCAGGACGGTGCGGCTGGGGTTCCAGACCAGTCAGAGCAGCGTGCCCACGCTGAGTGAGACATATTCTGTTTCGATTGCTGTTCTTCCGGTTTTTTTCAACGGGAAATTTACg                          >  8:174070/1‑135 (MQ=255)
                                                                                                            cagGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATg                       <  2:199557/136‑1 (MQ=255)
                                                                                                            cagGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCGGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATg                       <  3:242773/136‑1 (MQ=255)
                                                                                                            cagGGCAATTTCCATGTGAGGCAGGCCGGGGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATg                       <  4:72918/136‑1 (MQ=255)
                                                                                                             agGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATgc                      <  4:256862/136‑1 (MQ=255)
                                                                                                             agGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATgc                      <  3:161775/136‑1 (MQ=255)
                                                                                                                 ccATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATgccgcc                  <  5:160048/136‑1 (MQ=255)
                                                                                                                      tCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCTCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAg              <  3:148989/135‑1 (MQ=255)
                                                                                                                            tgAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTc        <  8:102331/135‑1 (MQ=255)
                                                                                                                            tgAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTc        <  8:198481/135‑1 (MQ=255)
                                                                                                                            tgAGGCAGGCAGGCGCGGCGTGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTc        <  3:7790/135‑1 (MQ=255)
                                                                                                                             gAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTCgg      >  3:213275/1‑136 (MQ=255)
                                                                                                                                 caggcCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTCGGTCGa  >  2:111755/1‑136 (MQ=255)
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GGTGCTCAGGCGGGGGCGGGGGACGGCGTGTGGGCCTTCTTCCATATAACGACGGATGAATTCCCACTCATCACGCAGTTCGCTGCCGCCTTGGCCTTGTTTGCCCAGCAGGGCCATTTCCATGTGAGGCAGGCCGGTGCGGCTGGGGTGCCAGACCAGGCCGAGCCGCGTGCCCACGCTGAGTGAGTCATCTTCTGGGTCGATGGCTGTGCTTCCGGTTGTTTTCCACGGGAAAGTGACGATGCCGCCGCAGTATTTCGGTCGA  >  ACB143/2353426‑2353690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑