Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,434,427 T→C 100% R175R (CGT→CGC)  topA → DNA topoisomerase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,434,4270TC88.7% 131.0 / 5.2 44R175R (CGT→CGC) topADNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base C (16/23);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCCGCGC  >  ACB143/2434295‑2434558
                                                                                                                                    |                                                                                                                                   
ggCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTc                                                                                                                                  >  6:59013/1‑136 (MQ=255)
        ggggTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACcg                                                                                                                          >  4:84452/1‑136 (MQ=255)
           gTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGt                                                                                                                       <  6:107706/136‑1 (MQ=255)
           gTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGt                                                                                                                       <  7:20714/136‑1 (MQ=255)
           gTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGt                                                                                                                       <  3:187747/136‑1 (MQ=255)
                 acacCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCgg                                                                                                                 >  8:166294/1‑136 (MQ=255)
                  cacCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGt                                                                                                                       <  1:234083/129‑1 (MQ=255)
                  cacCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGt                                                                                                                       >  2:234083/1‑129 (MQ=255)
                  cacCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGt                                                                                                                <  3:245234/136‑1 (MQ=255)
                      cGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACa                                                                                                            >  2:196921/1‑136 (MQ=255)
                           cAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATgg                                                                                                         <  2:271363/134‑1 (MQ=255)
                            aaGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGGCGGTTACATGGttt                                                                                                      <  5:255295/136‑1 (MQ=255)
                                    ggtgTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCActg                                                                                              >  3:32025/1‑136 (MQ=255)
                                    ggtgTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCActg                                                                                              >  2:131222/1‑136 (MQ=255)
                                    ggtgTTCAACGAAATTAACAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGATGGACATCGACCGGGTCAATGCCAAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCActg                                                                                              >  7:197248/1‑136 (MQ=255)
                                     gtgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCActg                                                                                              <  3:11456/135‑1 (MQ=255)
                                      tgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCActgct                                                                                            >  8:41151/1‑136 (MQ=255)
                                      tgtTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCActgct                                                                                            >  1:133922/1‑136 (MQ=255)
                                           aaCGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTggg                                                                                       >  1:251875/1‑136 (MQ=255)
                                                          aagGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTg                                                                        <  1:221403/136‑1 (MQ=255)
                                                          aagGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTg                                                                        <  8:61534/136‑1 (MQ=255)
                                                          aagGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTg                                                                        <  6:178962/136‑1 (MQ=255)
                                                            ggCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGt                                                                      <  4:74597/136‑1 (MQ=255)
                                                                 ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTc                                                                 <  2:155652/136‑1 (MQ=255)
                                                                 ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTc                                                                 <  8:188426/136‑1 (MQ=255)
                                                                 ttCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTc                                                                 <  4:263154/136‑1 (MQ=255)
                                                                    aGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCGCAGCAGGCGCGGCGTTTCCTCGAGCGAGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGc                                                              <  5:174667/136‑1 (MQ=255)
                                                                    aGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGc                                                              <  6:114821/136‑1 (MQ=255)
                                                                          ccTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCg                                                        <  8:210293/136‑1 (MQ=255)
                                                                           cTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCgt                                                       <  7:211146/136‑1 (MQ=255)
                                                                                 gCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAg                                                 >  6:42814/1‑136 (MQ=255)
                                                                                 gCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAg                                                 >  4:121240/1‑136 (MQ=255)
                                                                                       aGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTg                                           >  4:168674/1‑136 (MQ=255)
                                                                                         gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGc                                         <  3:84452/136‑1 (MQ=255)
                                                                                         gCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGc                                         <  8:84675/136‑1 (MQ=255)
                                                                                          cGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCg                                        <  4:82233/136‑1 (MQ=255)
                                                                                          cGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCg                                        <  8:51369/136‑1 (MQ=255)
                                                                                                 gACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAg                                  <  7:189188/135‑1 (MQ=255)
                                                                                                    aTCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTc                                                                 >  4:79644/1‑101 (MQ=255)
                                                                                                    aTCGACCGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTc                                                                 <  3:79644/101‑1 (MQ=255)
                                                                                                         ccGGGTCAATGCCCAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGGGAAGCTGGTGGtt                         >  8:76818/1‑136 (MQ=255)
                                                                                                                     ccAGCAGGCGCGGCGCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTTCAGTCGGTGGCGGTGCAGCTGGGGGTtgagcgtgagcgg             >  4:78264/1‑135 (MQ=255)
                                                                                                                                ggcgTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCCgcgc  <  7:166294/136‑1 (MQ=255)
                                                                                                                                ggcgCTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCCgcgc  <  5:244990/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GGCCATCGGGGGTGACGACACCCGCTACAAGCGCGTGGTGTTCAACGAAATTACCAAGAAGGCCATTCAGGAAGCCTTCTCGCAGCCAGGCGAGCTGGACATCGACCGGGTCAATGCCCAGCAGGCGCGGCGTTTCCTCGACCGCGTGGTCGGTTACATGGTTTCGCCACTGCTGTGGGCCAAGATTGCCCGTGGTCTGTCCGCTGGCCGTGTACAGTCGGTGGCGGTGAAGCTGGTGGTTGAGCGTGAGCGTGAGATCCGCGC  >  ACB143/2434295‑2434558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑