Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,883,291 C→A 11.8% T420N (ACC→AAC)  gabP_2 → GABA permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,883,2910CA11.8% 105.4 / 5.1 34T420N (ACC→AAC) gabP_2GABA permease
Reads supporting (aligned to +/- strand):  ref base C (18/12);  new base A (2/2);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

CCTGATGAACACCACCGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCG  >  ACB143/2883157‑2883423
                                                                                                                                      |                                                                                                                                    
ccTGATGAACACCACCGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGacc                                                                                                                                     >  8:32211/1‑136 (MQ=255)
       aaCACCACCGGCATGTTCGGCCTGCTTGTGTACCTGGTCATCTCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCGTATGTCAAGGAAGTACGCCTTATGATGTGTCTGTTCCCGTTTCTGacctaacttg                                                                                                                              <  2:47430/136‑3 (MQ=255)
             accGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATcg                                                                                                                        >  1:35438/1‑136 (MQ=255)
             accGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATcg                                                                                                                        >  8:105830/1‑136 (MQ=255)
             accGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATcg                                                                                                                        >  7:9196/1‑136 (MQ=255)
               cGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATcgcg                                                                                                                      >  6:193443/1‑136 (MQ=255)
                 gCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGtt                                                                                                                    <  2:225761/136‑1 (MQ=255)
                 gCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGtt                                                                                                                    >  1:180116/1‑136 (MQ=255)
                               ctgGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGccc                                                                                                      <  8:82706/136‑1 (MQ=255)
                                  gtgtACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCt                                                                                                     <  4:256058/134‑1 (MQ=255)
                                  gtgtACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTgg                                                                                                   >  1:251398/1‑136 (MQ=255)
                                  gtgtACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTgg                                                                                                   >  6:130751/1‑136 (MQ=255)
                                  gtgtACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCAGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCt                                                                                                     <  6:231269/134‑1 (MQ=255)
                                       ccTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCAcc                                                                                              <  1:247757/136‑1 (MQ=255)
                                       ccTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCAcc                                                                                              <  5:101105/136‑1 (MQ=255)
                                            tCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGAACTACCTGGTGATCGCGtt                                                                                                                    <  6:247773/109‑1 (MQ=255)
                                            tCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGAACTACCTGGTGATCGCGtt                                                                                                                    >  5:243237/1‑109 (MQ=255)
                                            tCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGAACTACCTGGTGATCGCGtt                                                                                                                    <  6:243237/109‑1 (MQ=255)
                                            tCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGAACTACCTGGTGATCGCGtt                                                                                                                    >  5:247773/1‑109 (MQ=255)
                                               tCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCtt                                                                                      >  2:194885/1‑136 (MQ=255)
                                                cGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCtt                                                                                      >  3:186480/1‑135 (MQ=255)
                                                   cTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATg                                                                                  <  1:96549/136‑1 (MQ=255)
                                                               gcgcATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTAc                                                                         >  3:4482/1‑133 (MQ=255)
                                                                             aGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTc                                                        <  7:105830/136‑1 (MQ=255)
                                                                             aGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTc                                                        <  6:32382/136‑1 (MQ=255)
                                                                                tGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCAc                                                     >  7:103996/1‑136 (MQ=255)
                                                                                            gCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTgc                                         >  3:77898/1‑136 (MQ=255)
                                                                                                 gAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGcggcgg                                    <  4:127515/136‑1 (MQ=255)
                                                                                                   aGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTc                                  <  1:189446/136‑1 (MQ=255)
                                                                                                               gATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATg                      >  2:104768/1‑136 (MQ=255)
                                                                                                                       tGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTgtggt              >  7:89975/1‑136 (MQ=255)
                                                                                                                       tGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTgtggt              >  4:81026/1‑136 (MQ=255)
                                                                                                                            ccGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTCGCGATGGGTGTGGTGCAc          >  6:121705/1‑135 (MQ=255)
                                                                                                                             cGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCAc          >  7:117490/1‑134 (MQ=255)
                                                                                                                              gTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAg       <  2:194425/136‑1 (MQ=255)
                                                                                                                                 gCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATc    >  6:165138/1‑136 (MQ=255)
                                                                                                                                     acctacctGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCg  <  8:103996/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
CCTGATGAACACCACCGGCATGATCGCCCTGCTGGTGTACCTGGTCATCGCCTTCTCGCAGCTGCGCATGCGCCGCAAGCTGATTGCCGAGGGCAAGGAAGTACGCCTGAAGATGTGGCTGTTCCCGTGGCTGACCTACCTGGTGATCGCGTTCATCGTGGCTGCCCTGGTCACCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCG  >  ACB143/2883157‑2883423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑