Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	3,267,793	T→G	27.8%	L171V (TTG→GTG)	eamA →	putative amino‑acid metabolite efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,267,793	0	T	G	27.8%	48.6 / 4.6	40	L171V (TTG→GTG)	eamA	putative amino‑acid metabolite efflux pump
Reads supporting (aligned to +/- strand):  ref base T (12/16);  new base G (9/3);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 8.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTA  >  ACB143/3267670‑3267920                                                                                                                            |                                                                                                                                 aTCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTg                                                                                                                        <  8:181660/134‑1 (MQ=255)    ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGGGTTGTGGGGTg                                                                                                                   >  8:4523/1‑136 (MQ=255)        gCGAAGCCGGCGGCCATGTGCCCAGGCTGGCGGTGGTGCTGACCCTGTGTGGCGGTGCGAGGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCt                                                                                                               <  6:184862/136‑1 (MQ=255)         ctaaGACGTCGGAAATGTGCCCATGCTGGCGTTGGTGCTTACCCTGTGTGGCTGTGAGATGTGGGGCCTGGGCAAAATCATTACACTACGGTTCGGCTCGTTCGACCTGTTCTCGTTTGTGAATTGTGGTggg                                                                                                                 >  4:240658/3‑133 (MQ=255)         cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATATGGGGTGGGCt                                                                                                               >  2:209867/1‑135 (MQ=255)              aCGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATAcc                                                                                                         >  2:46318/1‑136 (MQ=255)              aCGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGGTGATAcc                                                                                                         >  2:65411/1‑136 (MQ=255)                   ggcCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGGTCTGGGGTGGGCTGGTAACGCCa                                                                                                     >  4:252773/1‑135 (MQ=255)                    gcCATGTGCCCATGCTGGCGTTGGGGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTTGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACt                                                                                                   <  7:68075/136‑1 (MQ=255)                              ctggcgggCGGTGGTGGGGTCGCTGTGTGGGGGGGGGTGGGGGGGCGTGGGGCCCATCAGTCCCCGTCGGGTCGGGTCGGTCGACCTGGTCGCGGTGGGGAGCTGGGGGGGGCTGCTGCCGGCACTTCCATTCCTg                                                                                         <  6:207665/130‑1 (MQ=255)                              cATGCTGGCGTTGGTGCTGACCGTGTGGGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                         <  1:67125/136‑1 (MQ=255)                              cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                         <  8:52566/136‑1 (MQ=255)                              cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                         <  3:99950/136‑1 (MQ=255)                              cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                         <  3:128184/136‑1 (MQ=255)                              cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                         <  1:46318/136‑1 (MQ=255)                              cATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTg                                                                                         <  1:71414/136‑1 (MQ=255)                                           gTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCtgg                                                                             <  3:168114/135‑1 (MQ=255)                                                         tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTTGTGTTCTGGGGTGGGCTGATACCGCCACTTACATTCCTTGGACTGTCCTGGTGGCTGGAAGGcccc                                                              >  3:248933/1‑136 (MQ=255)                                                         tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGccc                                                               <  4:262991/135‑1 (MQ=255)                                                         tgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGCTCTGGGGTGGGCTGATACCGCCCCTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGgccc                                                              >  5:288012/1‑136 (MQ=255)                                                          ggCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTGGGTGATCTGGGGTGGGGTGATACCGCCCCTTCCATTGCTGGCACTGTCCTGGTGGCTGGAAGGCCCCg                                                             >  6:156596/1‑136 (MQ=255)                                                             ggTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                          <  5:147581/136‑1 (MQ=255)                                                              gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATGTGGGGTGGGCTGTTACAGCCACATCCATTACAGGCCCTGTCCTGGTGGCGGGAAGGCCCCCAgcg                                                         >  2:123265/1‑136 (MQ=255)                                                                  gATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGGTAACCCCACATACATTCCTGGCACTGTCCAGGTGGCTGGAAGGCCCCCAGCGCAtt                                                     >  2:43931/1‑136 (MQ=255)                                                                  gATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACTTGCATTCCTGGCACTGTCCTGGTGGCTGGGAGGCCCCGCGCGCAtt                                                     >  4:83750/1‑136 (MQ=255)                                                                    tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTGGGTGAGCAGGGGTGGGGGGCTACCGGCCCGTGCATTGCAGGGACTTTCCTGGTGGCGGGAAGGCGCCGCGCCCAATgt                                                   >  6:14085/1‑135 (MQ=255)                                                                    tgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGGGGGGGATATGGGGTTGGGTGAAAACGCCACATACAATCCGGGCACGGTCCAGGTGGGTGGAAGGCCCCGAGCGCaattg                                                   >  4:292446/1‑132 (MQ=255)                                                                        gggCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                               <  5:19952/136‑1 (MQ=255)                                                                        gggCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                               <  3:63134/136‑1 (MQ=255)                                                                        gggCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                               <  3:138506/136‑1 (MQ=255)                                                                                 cAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATAACCCCCCTTACATTACTGGCACTGTACAGGTGGCTGGAAGGCCCCGAGCGCATTTGCCAA‑GCCATGGc                                      >  6:115273/1‑135 (MQ=255)                                                                                 cAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATATGGGGTGGGCTGATACCGCCCCTTGCATTGCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGc                                      >  2:163992/1‑135 (MQ=255)                                                                                       catTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGCCAatat                                >  1:161048/1‑135 (MQ=255)                                                                                        atTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGCCAATATc                               <  4:286613/135‑1 (MQ=255)                                                                                            cccGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGGTGATACCGCCACTTCCATTCCTGGCACGGTCCTGGTGGGTGGAAGGCCCCGAGCGCATTGGCCCT‑GCCGTGGCCAATATCAGTTg                          >  5:267946/1‑136 (MQ=255)                                                                                            cccGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCAGGGGTGGGCGGATACCGCCACATACAATCCAGGCACTGTCCAGGTGGCTGGAATGCCCCGAGCGCATTGGCCAA‑GCCATGGCCAATATAAGATg                          >  2:209270/1‑136 (MQ=255)                                                                                            cccGACGGTTCGGCTCGGTCGACCTGGTCGCGGGGGGGCTGTGGGGTGGGGGGATACCGCCCCGGCAATTACTAGCACAGTCATGGGTGGGGGTAGGCCCCCCGCGCATTTGGCATGGCTTTGGCCAA‑ATCAGTTg                          >  2:299879/1‑136 (MQ=255)                                                                                             ccGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCAGGGGTGGGGGGCTACCGCCACTTCCAATCCAGGGACTGTGCGGGTGGGGGGAAGGCCCCCCGCGCAATGGCCCT‑GCCAAGGCCAAAATAAGTTgg                         >  4:229621/1‑136 (MQ=255)                                                                                              cGACGGTTCGGCTCGGTCGACCTGGTCGCGGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGCCAATATCAGTTGGa                        <  2:206993/136‑1 (MQ=255)                                                                                                                    tGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTa  <  5:12823/136‑1 (MQ=255)                                                                                                                            |                                                                                                                                 ATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGCGTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAT‑GCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTA  >  ACB143/3267670‑3267920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑