Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,448,459 C→T 9.2% A158T (GCC→ACC)  acsA_3 ← Acetyl‑coenzyme A synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,448,4590CT 9.2% 133.7 / 2.6 44A158T (GCC→ACC) acsA_3Acetyl‑coenzyme A synthetase
Reads supporting (aligned to +/- strand):  ref base C (20/18);  new base T (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTGCCTGCATACTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCGGCATGGGTGAACA  >  ACB143/3448327‑3448590
                                                                                                                                    |                                                                                                                                   
ccTTGCCTGCATACTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAg                                                                                                                                  <  5:89417/136‑1 (MQ=255)
ccTGGCCTGCATACTGGTAACCGGCGCAGACGAGCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGCTGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAg                                                                                                                                  <  1:260984/136‑1 (MQ=255)
      cTGCATACTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGTGAGCATGGc                                                                                                                            >  7:151742/1‑136 (MQ=255)
      cTGCATACTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGTGAGCATGGc                                                                                                                            >  7:148184/1‑136 (MQ=255)
            aCTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAg                                                                                                                      >  2:139268/1‑136 (MQ=255)
             cTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAgt                                                                                                                     >  3:62172/1‑136 (MQ=255)
             cTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAg                                                                                                                      <  4:165276/135‑1 (MQ=255)
                gTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGtt                                                                                                                  <  4:62172/136‑1 (MQ=255)
                 tAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGttt                                                                                                                 <  4:153374/136‑1 (MQ=255)
                 tAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGttt                                                                                                                 >  1:60736/1‑136 (MQ=255)
                    ccGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGcc                                                                                                              <  7:280333/136‑1 (MQ=255)
                      ggCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAg                                                                                                            >  1:25536/1‑136 (MQ=255)
                      ggCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAg                                                                                                            >  1:258762/1‑136 (MQ=255)
                        cgcAGACGATCAGCAGCTTGGGTGCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGt                                                                                                          <  5:220420/136‑1 (MQ=255)
                           aGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTAtt                                                                                                       >  6:33415/1‑136 (MQ=255)
                                             gTTCGATCTGGCCGAATCGGTCGATGAAGAAGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGAGAGCATTGCCACCAGGGTTTGCAAGGGATTGGTCATGATGGCGGCTAc                                                                                     >  5:187946/1‑136 (MQ=255)
                                              ttCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTAcc                                                                                    >  3:40202/1‑136 (MQ=255)
                                              ttCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTAcc                                                                                    >  1:150584/1‑136 (MQ=255)
                                                   tCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTc                                                                               <  4:177323/136‑1 (MQ=255)
                                                     tGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGc                                                                             >  5:193339/1‑136 (MQ=255)
                                                       gCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTc                                                                               <  2:12135/132‑1 (MQ=255)
                                                          gAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCgg                                                                         >  5:263459/1‑135 (MQ=255)
                                                                    atgatgCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGAtaccta                                                              >  2:165506/1‑136 (MQ=255)
                                                                                aCGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATg                                                  >  1:194080/1‑136 (MQ=255)
                                                                                  gCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGcc                                                >  1:46538/1‑136 (MQ=255)
                                                                                        cTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGc                                          <  4:280722/136‑1 (MQ=255)
                                                                                                   aggTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCAc                               >  5:137679/1‑136 (MQ=255)
                                                                                                    ggTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCAcc                              <  4:144355/136‑1 (MQ=255)
                                                                                                         accagaccGCGCCAAGGCTGGTGCAGGTGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCTGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGc                         <  8:151742/136‑1 (MQ=255)
                                                                                                         accagaccGCGCCAAGGCTGGTGCAGGTGAGCATGGCCACCAGTGGTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGGCGCCCGGGACGATACCTACCGCCTTGAATGCCGGTTGCAGGCCGGCCACCTGTGc                         <  8:148184/136‑1 (MQ=255)
                                                                                                          ccagaccGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGGGTTTGCCAGGTAGTGGGCATGATGGCGGCTACCCGGGCGCCCGGGACGATCCCTAACGCCTTGAATTCCGTGGGCATGCAGGCCACCTGTGCg                        >  1:125986/1‑136 (MQ=255)
                                                                                                            agaccGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGc                      <  4:10781/136‑1 (MQ=255)
                                                                                                            agaccGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCAGGGACGATACCTACCGCCTTGAATGCAGATTGCAGGCCGGCCACCTGTGCCGc                      >  6:10500/1‑136 (MQ=255)
                                                                                                              accGCGCCAAGGCTGGGGCAGGCGAGCATGGCCACCAGTGTTGGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCa                    <  4:33972/136‑1 (MQ=255)
                                                                                                                 gcgcCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGttt                                                                                                                 <  4:153960/40‑1 (MQ=255)
                                                                                                                 gcgcCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGttt                                                                                                                 >  3:153960/1‑40 (MQ=255)
                                                                                                                 gcgcCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGtt                 <  7:254378/136‑1 (MQ=255)
                                                                                                                   gcCAAGGCTGGTGCATGAGACCATGGCCACCTGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATTCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCg               <  5:204100/136‑1 (MQ=255)
                                                                                                                   gcCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCg               <  1:213788/136‑1 (MQ=255)
                                                                                                                     cAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCAGTGCGGCCAGTTCgg              >  8:212880/1‑135 (MQ=255)
                                                                                                                           tGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCGGCATGGGt       >  8:246906/1‑136 (MQ=255)
                                                                                                                             gTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCGGCATGGGt       <  3:115383/134‑1 (MQ=255)
                                                                                                                                cAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCGGCATGGGTGAaca  >  2:38222/1‑136 (MQ=255)
                                                                                                                                cAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCGGCATGGGTGAac   <  4:292717/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CCTTGCCTGCATACTGGTAACCGGCGCAGACGATCAGCAGCTTGGGTTCGATCTGGCCGAAGCGGTCGATGATGCCGTGCACGCCGAACTCGGGCGAGGAGGTAGACCAGACCGCGCCAAGGCTGGTGCAGGCGAGCATGGCCACCAGTGTTTGCCAGGTATTGGGCATGATGGCGGCTACCCGGTCGCCCGGGACGATACCTACCGCCTTGAATGCCGTTTGCAGGCCGGCCACCTGTGCGGCCAGTTCGGCATGGGTGAACA  >  ACB143/3448327‑3448590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑