| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 4,759,509 | G→A | 100% | intergenic (+124/‑16) | fpvA_3 → / → ligM_KToptimized | Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,759,509 | 0 | G | A | 98.9% | 338.2 / ‑4.2 | 94 | intergenic (+124/‑16) | fpvA_3/ligM_KToptimized | Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440 |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (47/46); total (48/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTC > ACB143/4759376‑4759638 | cAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGt < 3:4293/136‑1 (MQ=255)cAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGt < 2:40739/136‑1 (MQ=255) aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTATGAGCTGTTGACAATTAATCATCGGCTCTTATAATGTGTGGAATTGTGAGCGTATAACAATTTCACACCAACACATAGAGtt > 8:147562/1‑136 (MQ=255) gattCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 6:244781/134‑1 (MQ=255) gCTTCTGCTCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 8:79509/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 5:175655/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 3:190725/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 3:83106/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 4:238340/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 2:79135/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 2:79134/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 5:62531/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 2:118984/136‑1 (MQ=255) gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCAACCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt < 3:213196/136‑1 (MQ=255) ttCTGATCGCCTGACGTTGAACGCAAAAATCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGAAAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag < 8:210904/136‑1 (MQ=255) ttCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag > 8:69579/1‑136 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag > 1:198287/1‑134 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag > 7:229473/1‑134 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag > 4:143164/1‑134 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag > 7:4773/1‑134 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag > 3:227097/1‑134 (MQ=255) cTGATAGCATGACGTTGAAAGCAAAAAACCGCACCCAGGTTCGGTTTTTTGATCTGTTGACAATTAATCATCGGCTCGTATAATGTGTTGAATTGTGAGCGGATAACAATTTCACAGCAACACATAGAGTTTag < 4:183923/134‑1 (MQ=21) aTCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTaggag < 3:270688/134‑1 (MQ=255) cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGta > 3:190540/1‑135 (MQ=255) ccTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATAc < 7:82915/136‑1 (MQ=255) cTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACa > 4:130778/1‑136 (MQ=255) gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACAt > 6:77350/1‑135 (MQ=255) gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACAt > 4:239328/1‑135 (MQ=255) ctttGAACGCAAAACACCGCACCCAGCTGCGGTTTTTTGAGCTGGTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAg < 1:49560/134‑1 (MQ=25) ttGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgcg > 2:38838/1‑136 (MQ=255) gAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGcc > 2:173546/1‑136 (MQ=255) aaCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGccc < 8:11563/136‑1 (MQ=255) aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGcccc < 1:111456/136‑1 (MQ=255) cGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCg < 6:269930/136‑1 (MQ=255) aaaaaaCCGCACCCAGGGGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACt < 6:172323/136‑1 (MQ=255) aaaaaCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTa < 7:195670/136‑1 (MQ=255) aaaaaCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTa < 8:246527/136‑1 (MQ=255) aaaCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAAc > 6:276183/1‑136 (MQ=255) ccGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTg < 4:241206/136‑1 (MQ=255) cGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTgg < 4:126674/136‑1 (MQ=255) cAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAgg > 3:217465/1‑136 (MQ=255) cGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTgg < 7:69579/136‑1 (MQ=255) gTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGgcc < 8:210610/136‑1 (MQ=255) aGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGTATAACAATTTCACACCAACACATAGAGTTTAGGCGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggcgg < 6:199208/136‑1 (MQ=255) aGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGAAAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggcgg < 3:188965/136‑1 (MQ=255) cTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCa < 5:156116/136‑1 (MQ=255) ttGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAaca < 5:248538/136‑1 (MQ=255) aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCGACACCg > 3:38700/1‑136 (MQ=255) aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg > 7:119319/1‑136 (MQ=255) aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACATGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg > 5:259716/1‑136 (MQ=255) aCAATTAATCATCGGCGCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg < 3:8724/136‑1 (MQ=255) atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt > 5:29547/1‑136 (MQ=255) atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt > 4:2727/1‑136 (MQ=255) tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg < 7:62750/136‑1 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAaca > 1:205977/1‑118 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAaca < 2:205977/118‑1 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGt > 6:51785/1‑135 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGt > 7:129318/1‑135 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGt > 7:165830/1‑135 (MQ=255) aTGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCa < 7:122977/135‑1 (MQ=255) tgtgtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGa < 1:75707/136‑1 (MQ=255) tgtgtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGa < 4:217465/136‑1 (MQ=255) tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTgg > 8:24984/1‑136 (MQ=255) ggAATTGTGAGCGGATAACAATTTCACTCCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc > 5:62470/1‑136 (MQ=255) gAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc < 4:27737/135‑1 (MQ=255) gAATTGTGAGCGGATAACAATTTCACACAAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc < 3:278818/135‑1 (MQ=255) gAATTGTGAGCGGATAACAATTTCACACAAACACATACAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc < 7:185582/135‑1 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGATTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 3:292891/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACCCCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 5:167318/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 2:114141/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 5:230675/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 2:96530/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 6:277603/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 5:116533/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc > 5:98016/1‑136 (MQ=255) aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgagc > 5:18814/1‑136 (MQ=255) gtgAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTa < 5:28157/135‑1 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc > 3:270069/1‑135 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc > 6:119064/1‑135 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc > 4:122482/1‑135 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc > 7:71608/1‑135 (MQ=255) gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc > 5:250982/1‑135 (MQ=255) ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTGCCAGATTGGCGCCTACGTCTAc < 4:67260/136‑1 (MQ=255) ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAc > 7:12182/1‑136 (MQ=255) ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAc > 2:8880/1‑136 (MQ=255) tAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCg > 4:216033/1‑136 (MQ=255) cacaCCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCACCACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTcgcg > 6:79029/1‑136 (MQ=255) cacaAACACATCGAGTTTAGCAGGAAAACAAGCGCGCCCCCCCAAACCTGGCGCAGGTGGGGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGcc < 5:106796/136‑1 (MQ=255) acCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCgcgc > 3:57605/1‑134 (MQ=255) cAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCa > 2:290096/1‑134 (MQ=255) cacaTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGtt > 3:192254/1‑136 (MQ=255) acaTAGAGATTAGGAGCTATCCATCAGCGCCCCGACTAACCTGGAGCCGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtc < 7:262209/136‑1 (MQ=255) caTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGtcgtcg > 6:140881/1‑124 (MQ=255) caTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGtcgtcg < 5:140881/124‑1 (MQ=255) | CAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTC > ACB143/4759376‑4759638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |