Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,788,936 A→C 14.7% V230G (GTG→GGG)  dat ← Diaminobutyrate‑‑2‑oxoglutarateaminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,788,9360AC14.7% 201.9 / 3.7 120V230G (GTG→GGG) datDiaminobutyrate‑‑2‑oxoglutarateaminotransferase
Reads supporting (aligned to +/- strand):  ref base A (64/34);  new base C (15/7);  total (79/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGC  >  ACB143/4788802‑4789067
                                                                                                                                        |                                                                                                                                    
ggATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCAc                                                                                                                                     >  6:29650/1‑136 (MQ=255)
    ctcGTCGACGATCAACGCCACGCCCGCCTGCTCGCTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACATCGAGGATCACGGCGGCGGGCAGCGGCAcgccg                                                                                                                                 >  5:282166/1‑136 (MQ=255)
         cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCACCCCGCTTTc                                                                                                                            >  3:50610/1‑136 (MQ=255)
         cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTc                                                                                                                            >  5:52831/1‑136 (MQ=255)
          gacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTcc                                                                                                                           <  3:121503/136‑1 (MQ=255)
          gacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTcc                                                                                                                           >  2:86607/1‑136 (MQ=255)
              aTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGAt                                                                                                                       >  7:74531/1‑136 (MQ=255)
                    gccacgccCGCCTGCGCGGTAATGCGCCGCACGCCCTTGAGCAATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGTATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGagca                                                                                                                 <  2:59930/136‑1 (MQ=255)
                            cgccTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGttt                                                                                                         >  4:118874/1‑136 (MQ=255)
                               cggggcGGTATTGCGCCCCCCGCCCTTTAGCATTTGTTTGCCGGCCGGAACCACCCCGCCCTCACCGTCCACCACTTCGTGTTTCACGGGGGGGGGCTGGGGCAGGCCGCTTTCCGGATCGAGCAGCAGGTTTTcc                                                                                                      <  4:279451/131‑1 (MQ=255)
                                tGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCCCTTTCCGGAGAGCGCAGCAGGtttta                                                                                                       >  6:174266/1‑133 (MQ=255)
                                 gCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCCGCAGGTTTTGCAg                                                                                                    >  4:510/1‑136 (MQ=255)
                                        aaTGCGCCGCACGCCGTTGAGCATTTCGATGTCGTCCGGACCCCCCCCGCCCTCACCCTGCCCCAATTGGATGATCACGGCGGGGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGc                                                                                             <  2:71878/136‑1 (MQ=255)
                                         ttGCGCGCCACGCCCTTCAGCAATTAGATGTCGGACGTAACCACCCCGCCCTCACCCTGCACCACTTCTAGGATCACGGCGGCGGGCTGCGGCACGCCGCTTTCCGGATCGAGCATCAGGTTTTCCAGGTAATGc                                                                                             <  6:266228/134‑1 (MQ=255)
                                         aTGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGc                                                                                             <  3:226574/135‑1 (MQ=255)
                                          tGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCCGCAGGTTTTCCAGGTAATGCAg                                                                                           >  6:102752/1‑136 (MQ=255)
                                               cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCCGGTAATGCAGGTTgg                                                                                     >  8:211535/1‑136 (MQ=255)
                                               cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTgg                                                                                     >  5:285151/1‑136 (MQ=255)
                                               cgcACGCCCTTGAGCCATTCGATGGCGGCCGGACCCACCCCGCCCTCCCCCTGCACCCATTCGAGGATCACGGCGGGGGGCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTgg                                                                                     <  1:223275/136‑1 (MQ=255)
                                                gcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCGGCGAGCGCAGCAGGTTTTGCAGGTAATGCAGGTTGGc                                                                                    >  5:261047/1‑136 (MQ=255)
                                                  aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACACAGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGCTCGAGCAGCAGGTTTTCCAGGTAAAGCAGGTTGGCCt                                                                                  >  2:271785/1‑136 (MQ=255)
                                                   cccccTGGAGCCATTCGAGGGCGGCCGCAACCACCCCCCCCTCCCCCTGCCCCACTTCGCGGAGCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                 <  7:257370/134‑1 (MQ=255)
                                                    gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                 >  5:52386/1‑135 (MQ=255)
                                                    gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                 >  5:212106/1‑135 (MQ=255)
                                                    gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                 >  5:124553/1‑135 (MQ=255)
                                                    gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTg                                                                                >  1:157225/1‑136 (MQ=255)
                                                    gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGCATCACGGCGGCGGGCAGCGGCACGCCGCGTTACGGATAGCGCCGCATGTTTTACAGGTAATGGACGTTGGCCtt                                                                                 >  3:43954/1‑135 (MQ=255)
                                                    gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGACCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCCtt                                                                                 >  6:53527/1‑135 (MQ=255)
                                                         tGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGAcccc                                                                           >  2:164188/1‑136 (MQ=255)
                                                          gAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGAccc                                                                            <  6:285151/134‑1 (MQ=255)
                                                            gCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTACTGATGGAGCAGCAGGTTTTACAGGTAATTCAGGTTGGCCATTACCCCGGc                                                                        >  2:154782/1‑136 (MQ=255)
                                                             ccATTCGAGTTGGGCCGTAACAACCCCGCCATCCCCGGCAACCATTTCTCGGATCCCGGCGGGGGGCAGCGGCCCGCGGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGcc                                                                       <  2:116324/136‑1 (MQ=255)
                                                             ccATACGATGGGGGCCGTAACCACCCCGCCCTCACCCTGCACAAGTGCTAGTATCCCGGGGGCGGGCCGCGCCACCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGcc                                                                       <  2:235704/136‑1 (MQ=255)
                                                                   gATGTCGGCCGGAACCCCCCCGCCCTCACCCTGCACCACTTCGAGGATCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 <  8:272743/136‑1 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCACGCCGGGTTCCGGATCGAGCAGCAGGTTTTGCAGGGAATGCAGGTTTGGCTTTCACCCCGCCTCACCg                                                                 >  7:243374/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  3:16961/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTGCGGATCGAGCCGCAGGGTTTACAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  4:47889/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTGCAGGTAATTCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  2:125226/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  1:26335/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGCTCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  4:103577/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  7:213753/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  1:193103/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  1:209894/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  1:44204/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  3:231713/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCCCGTTCCGGAGACAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCACACCg                                                                 >  6:32660/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGCCCCCCGCCACACCg                                                                 >  2:109007/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                 >  5:120067/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACCCCGCGTTGCGGCTCGAGCAGCAGGGTTTGCAGGTAATGCAGGTTGGCCTTGACCCCGGCCGCCCCg                                                                 >  3:196149/1‑136 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGCCACGCCGCTTACCGGATCGAACAGCACGTTTTACACGTAATGCAGGTTGGCCATGACCCCCGCCTCAccc                                                                 >  7:149933/1‑135 (MQ=255)
                                                                   gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACGTCGAGGCTCACGGCGGCGGGCAGCGGCACGCCGCTTTCGGGATCGAGCCGCAGGTTTTAGAGGGAATGCAGGTTGGGCTTGACCCCGGCCTCACCg                                                                 >  6:199332/1‑136 (MQ=255)
                                                                             ggAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTGACCCCGGCCACACCGCCCAGGCCgc                                                       >  4:2581/1‑135 (MQ=255)
                                                                             ggAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCg                                                        >  8:49126/1‑135 (MQ=255)
                                                                                 ccaccCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCCCTTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCCATGACCCCCGCCTCACCGCCCAGGCCGAACgg                                                   >  2:154336/1‑136 (MQ=255)
                                                                                  caccCCGCCCGCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACggg                                                  <  2:14304/136‑1 (MQ=255)
                                                                                   cccccGCCCCCCACCCTGCCCCAGTACGCGGAGCCCGGCGGGGGGCACCGGACCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACggg                                                  <  6:199649/135‑1 (MQ=255)
                                                                                    ccccGCCCTCACCCTGCACCACTTCGAGGATCCCGGCGGGGGGCTGCGGCACGCGGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCGTCACCGCCCAGGCCGAACGGGCa                                                <  5:282707/136‑1 (MQ=255)
                                                                                      ccGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGc                                              <  8:207945/136‑1 (MQ=255)
                                                                                       cGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGCACGGGCAGCg                                             >  2:88878/1‑136 (MQ=255)
                                                                                       cGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCg                                             >  2:10960/1‑136 (MQ=255)
                                                                                             tCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCCCGTTACGGGTCGAGCACCAGGGTTTGCAGGTAATGAAGGTTGGGCATTACCCCCGCCTCACCCCCCCGGCCCCACGGGCCGCGGTAgg                                        >  4:11230/1‑135 (MQ=255)
                                                                                             tCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGt                                        >  4:245474/1‑136 (MQ=255)
                                                                                              cccccTGCACCACTTGCTGCACCCCGGCGGCGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                       <  1:109007/134‑1 (MQ=255)
                                                                                              cACCCTGCACCCCTTCGAGGATCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTCGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                       <  8:234270/136‑1 (MQ=255)
                                                                                              cACCCTGCACCACTTCGCGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                       <  5:87993/136‑1 (MQ=255)
                                                                                              cACCCTGCACCAATTCGAGCAGCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                       <  2:269539/136‑1 (MQ=255)
                                                                                               aCCCTCCCCCACTTGGCGCATCCCGGGGGCGGGCCGCGGCCCGCGGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGt                                        <  1:94426/134‑1 (MQ=255)
                                                                                                   tGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCGGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                  <  8:145702/136‑1 (MQ=255)
                                                                                                   ggCACCACTTCGAGGATCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                  <  1:235944/135‑1 (MQ=255)
                                                                                                     caccagTTCGCCGAGCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACggg                                <  8:177227/136‑1 (MQ=255)
                                                                                                     caccacTTCGCGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                  <  8:34890/134‑1 (MQ=255)
                                                                                                     caccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                  <  5:274449/134‑1 (MQ=255)
                                                                                                     caccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                  <  3:245474/134‑1 (MQ=255)
                                                                                                     caccacTGCGAGGATCACGGCGGGGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                  <  7:48352/134‑1 (MQ=255)
                                                                                                      ccccacTTCGCGGATCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAAAGGGCAGCGGTAGTCGTACg                                  <  1:259229/132‑1 (MQ=255)
                                                                                                        ccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCACGTTGGCCTTGACCCCGGCCTCACCGCCCCCGCCGCACCGGCAGCGGTAGTCGTAAGGGt                               >  1:124783/1‑135 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCCGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGCACGGGCAGCGGTAGTCGTACGGGt                               >  5:66957/1‑134 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                               >  3:131793/1‑134 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGttt                                                                                                         >  7:271256/1‑61 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGttt                                                                                                         <  8:271256/61‑1 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                               >  5:110744/1‑134 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                               >  2:91451/1‑134 (MQ=255)
                                                                                                         cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTTGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                               >  3:113667/1‑134 (MQ=255)
                                                                                                         cacTTAGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                               >  8:71858/1‑134 (MQ=255)
                                                                                                           cTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTACGGATCGAGCCGCAGGGTTTACAGGTAATTCAGGTTGGCCTTTACCCCCGCCTCACCCCCCCCGCCCCACCGGCAGCGGTAGTAGTACGGGTAAgg                           >  6:187860/1‑136 (MQ=255)
                                                                                                             tCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCa                         >  6:91828/1‑136 (MQ=255)
                                                                                                             tCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCa                         >  6:216122/1‑136 (MQ=255)
                                                                                                             tCGAGGATCACGGCGGCGGGCAGCGGCACCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCa                         >  7:269542/1‑136 (MQ=255)
                                                                                                              cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCCCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt                        >  4:17834/1‑136 (MQ=255)
                                                                                                              cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTGCGGATCGAGCAGCAGGTTTTGCAGGTAATTCAGGTTGGCCTTTACCCCCGCCTCACCGCCCACGCCGAACGGGCCGCGGGAGTCGTACGGGGTAGGCAt                        >  8:186584/1‑136 (MQ=255)
                                                                                                               gAGGATCACGGCGGCGGGCAGCCGCACGCCGCTTTCCGGATCGAGCATCAGGTTTTCAAGGTAATTCAGGTTGGCCTTGACCCCGGCCTCACCCCCCAGGCCGAACGGGCAGCCGTAGTCGTACGGGTAAGGCAt                        >  2:253693/1‑135 (MQ=255)
                                                                                                                 gtcccccgggggcggGCCGCGGCCGCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGGAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGaa                     <  4:188968/130‑1 (MQ=255)
                                                                                                                 ggATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGaa                     >  6:209642/1‑136 (MQ=255)
                                                                                                                    tcccggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACt                   <  2:243702/132‑1 (MQ=255)
                                                                                                                      ccggcgggggGCCGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCa                <  8:182800/135‑1 (MQ=255)
                                                                                                                        ggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACg              <  5:120575/136‑1 (MQ=255)
                                                                                                                           ggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCg           >  7:243/1‑136 (MQ=255)
                                                                                                                                  cgCGGCACGCCGCTTTCCGGAGCGAGCAGCAGGTTTTCCAGGTACTGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGa    <  1:268483/135‑1 (MQ=255)
                                                                                                                                  aGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGCCCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGcc      >  7:134130/1‑134 (MQ=255)
                                                                                                                                  aGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGa    <  7:74355/136‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
GGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGC  >  ACB143/4788802‑4789067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: