| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 546,499 | A→G | 89.8% | intergenic (+11/‑6) | rplB → / → rpsS | 50S ribosomal protein L2/30S ribosomal protein S19 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 546,499 | 0 | A | G | 89.8% | 113.9 / 4.3 | 40 | intergenic (+11/‑6) | rplB/rpsS | 50S ribosomal protein L2/30S ribosomal protein S19 |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (24/11); total (26/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01 | |||||||||||
GTGGTGGTGAAGGTCGTACCTCCGGTGGTCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATACGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTAAAACCTGGTCGCGCCGTTCGATGATCCTGCCA > ACB143/546364‑546630 | gtggtggtgAAGGTCGTACCTCCGGTGGGCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATa < 1:195609/136‑1 (MQ=255) gtggtgAAGGTCGTACCTCCGGTGGTCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGa > 4:280412/1‑136 (MQ=255) gtgAAGGTCGTACCTCCGGTGGTCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAg > 1:170853/1‑136 (MQ=255) gtgAAGGTCGTACCTCCGGTGGTCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAg > 4:251195/1‑136 (MQ=255) cGTACCTCCGGTGGTCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTATCTAGAGGGATGCGACAGTGCCACGt > 2:140250/1‑136 (MQ=255) gtcATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGAGAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTc > 2:258786/1‑136 (MQ=255) tCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCttt < 8:154941/136‑1 (MQ=255) tCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCttt < 8:229704/136‑1 (MQ=255) tCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCttt < 3:280412/136‑1 (MQ=255) ccGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCttt > 1:50242/1‑135 (MQ=255) tCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGAt > 6:73567/1‑136 (MQ=255) ccATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATACGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCtt < 3:105693/136‑1 (MQ=255) cTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGtt > 3:297256/1‑136 (MQ=255) caaccaaGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTgaag > 7:8735/1‑135 (MQ=255) caaccaaGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTg > 5:286234/1‑132 (MQ=255) aaccaaGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTgaag > 8:17008/1‑134 (MQ=255) aaccaaGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTgaag > 3:65561/1‑134 (MQ=255) aaccaaGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATACGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTgaag > 7:92691/1‑134 (MQ=255) aaccaaGGCTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCtt > 4:229711/1‑122 (MQ=255) aaccaaGGCTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCtt < 3:229711/122‑1 (MQ=255) aGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTc > 1:275093/1‑135 (MQ=255) tGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACATGTTGAAGAAGGTCGAAGt > 1:175553/1‑136 (MQ=255) cTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTg < 7:79320/135‑1 (MQ=255) aaaCCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCgg > 8:73512/1‑136 (MQ=255) ggTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAgtggaggtggag > 3:240243/1‑133 (MQ=255) ggTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGgag > 3:244297/1‑133 (MQ=255) taataaGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGTTCATTTTATCGATCTTCACATTTTGAAGATGGTCTATTGGGCGGTGGAgaagaa > 3:121759/1‑136 (MQ=255) gTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCa < 8:218889/136‑1 (MQ=255) gTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCa < 7:92180/136‑1 (MQ=255) gTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATACGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCa < 3:162642/136‑1 (MQ=255) cGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGc > 1:87128/1‑135 (MQ=255) acaTGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTtc > 7:265568/1‑53 (MQ=255) acaTGATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTtc < 8:265568/53‑1 (MQ=255) gATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTaaa < 2:136063/134‑1 (MQ=255) gATCGTCCGTCGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTaaa > 1:136063/1‑134 (MQ=255) ccgtcGTCGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTAAAACCTGGTc < 6:162985/136‑1 (MQ=255) cgtcgtCGCAATTAACTAGAGGGATTCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTAAAACCTGGTc < 3:197033/135‑1 (MQ=255) gtcgtcGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGaaa < 6:64868/48‑1 (MQ=255) gtcgtcGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGaaa > 5:64868/1‑48 (MQ=255) gtcgtcGCAAGTAACTAGAGGGATGCGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTAAAACCTGGTcgc > 6:210085/1‑136 (MQ=255) gATACGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTAAAAACCTGGTCGAGCCGTTCGATGATCCTGCca > 7:193909/1‑135 (MQ=255) | GTGGTGGTGAAGGTCGTACCTCCGGTGGTCGTCATCCGGTATCGCCATGGGGCTTCCCAACCAAGGGTGCTAAAACCCGTGGTAATAAGCGTACCGACAACATGATCGTCCGTCGTCGCAAGTAACTAGAGGGATACGACAGTGCCACGTTCTCTGAAAAAAGGTCCTTTTATCGATCTTCACCTGTTGAAGAAGGTCGAAGTGGCGGTGGAGAAGAACGATCGCAAGCCAGTTAAAACCTGGTCGCGCCGTTCGATGATCCTGCCA > ACB143/546364‑546630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |