Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	659,271	C→G	6.0%	R1029R (CGC→CGG)	yfhM →	Alpha‑2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	659,271	0	C	G	6.0%	233.4 / 6.2	67	R1029R (CGC→CGG)	yfhM	Alpha‑2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base C (24/39);  new base G (2/2);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

GAGCACTTCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGCCCAGGGAG  >  ACB143/659140‑659398                                                                                                                                    |                                                                                                                                gagCACTTCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGAGGCGACCGACCCCGCATGGCACTGGCCCTGGCCAACCTGTCCGGCCGCGccc                                                                                                                             >  5:231184/1‑136 (MQ=255)  agCACTTCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCTCGCTTCCTGGCCGGTGTCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGcc                                                                                                                              >  2:27906/1‑134 (MQ=255)  agCACTTCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGcc                                                                                                                              >  3:69347/1‑134 (MQ=255)  agCACTTCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGcc                                                                                                                              >  4:134142/1‑134 (MQ=255)       ttCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCaa                                                                                                                        >  8:124909/1‑135 (MQ=255)         cGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAAc                                                                                                                       <  4:65647/134‑1 (MQ=255)            cATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCATCCTGTCCGGCCGCGCCCAGCAACTgcgc                                                                                                                  >  1:215058/1‑136 (MQ=255)                    aaGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAAt                                                                                                          >  3:167180/1‑136 (MQ=255)                            aCCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACg                                                                                                   >  4:264168/1‑135 (MQ=255)                            aCCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACg                                                                                                   >  4:187135/1‑135 (MQ=255)                                   tCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGGGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGc                                                                                            >  6:162552/1‑135 (MQ=255)                                   tCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGGGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGc                                                                                            >  6:166024/1‑135 (MQ=255)                                   tCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGc                                                                                            >  5:141970/1‑135 (MQ=255)                                   tCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGATTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCAGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGc                                                                                            >  7:88634/1‑135 (MQ=255)                                    cgccgcgccgTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCCCTGGACCTGGCCACCCTGTGCGGCCGCGCCCTGCAACTGAGCGTCGAAAGCACCACGGATGGGCaa                                                                                          >  4:217166/1‑136 (MQ=255)                                    cgccgcgccgTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCaa                                                                                          >  3:126919/1‑136 (MQ=255)                                        gcgccgTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGCCCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACCGATGGGCACCt                                                                                        >  5:45948/1‑134 (MQ=255)                                          gccgTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGc                                                                                    <  8:219246/136‑1 (MQ=255)                                                gATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTggc                                                                               <  7:124909/135‑1 (MQ=255)                                                 aTTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTggcgg                                                                             <  5:163154/136‑1 (MQ=255)                                                 aTTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTggcgg                                                                             <  3:284992/136‑1 (MQ=255)                                                 aTTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTggcgg                                                                             <  8:144532/136‑1 (MQ=255)                                                 aTTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTggcgg                                                                             <  5:7223/136‑1 (MQ=255)                                                     ccGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCa                                                                          <  7:62208/135‑1 (MQ=255)                                                     ccGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCa                                                                          <  1:212399/135‑1 (MQ=255)                                                     ccGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCa                                                                          <  2:97256/135‑1 (MQ=255)                                                     ccGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCa                                                                          <  3:101060/135‑1 (MQ=255)                                                     ccGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCa                                                                          <  3:139167/135‑1 (MQ=255)                                                      cGAGCGTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCGGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGGCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAAc                                                                        <  7:206711/136‑1 (MQ=255)                                                         gCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACAGGACCTGGCCAACCTGTCCGGCCGCGCCCCGCAACTGAGCCTCGAAATCACCACGGATGGGCCACTGAGCCTGGCGGCCAACGcc                                                                     >  2:280451/1‑136 (MQ=255)                                                           tttCGGCCCCGCGCTTCCTGGCCGGTGGCGCCCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   >  4:261955/1‑136 (MQ=255)                                                           attCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACAGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   <  3:165494/135‑1 (MQ=255)                                                             tCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   <  5:47919/134‑1 (MQ=255)                                                             tCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   <  5:43994/134‑1 (MQ=255)                                                             tCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   <  1:15828/134‑1 (MQ=255)                                                             tCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   <  2:165534/134‑1 (MQ=255)                                                             tCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCGGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGt                                                                   <  5:105301/134‑1 (MQ=255)                                                             tCGGCACGGCGCGTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCATCTGAGCGTCGAAATCTCAACGGATGGGCAACTGAGCCTGGCTGCCAACGCCGt                                                                   <  8:258854/134‑1 (MQ=255)                                                                     gcgcTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGtt                                                         <  7:88234/136‑1 (MQ=255)                                                                            cTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGTCAACGCCGTGCAGAGCGTTAACCTgg                                                  <  4:167180/136‑1 (MQ=255)                                                                                 cGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGGGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGaa                                             <  5:166024/136‑1 (MQ=255)                                                                                 cGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGGGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGaa                                             <  5:162552/136‑1 (MQ=255)                                                                                      gCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTTACCTGGCCGAAGGGCa                                        >  4:156600/1‑136 (MQ=255)                                                                                        gACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGc                                      <  4:196613/136‑1 (MQ=255)                                                                                        gACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGc                                      <  8:149065/136‑1 (MQ=255)                                                                                        gACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGc                                      <  4:69347/136‑1 (MQ=255)                                                                                             tACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCg                                 <  3:204431/136‑1 (MQ=255)                                                                                               ccAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGAc                               <  1:154001/136‑1 (MQ=255)                                                                                               ccAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGAc                               <  3:288665/136‑1 (MQ=255)                                                                                                cAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACg                              >  6:254181/1‑136 (MQ=255)                                                                                                cAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACg                              >  6:90540/1‑136 (MQ=255)                                                                                                       gCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCtgatga                       <  8:229002/136‑1 (MQ=255)                                                                                                       gCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCtgatga                       <  5:163417/136‑1 (MQ=255)                                                                                                        cACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTgatgat                      <  8:93153/136‑1 (MQ=255)                                                                                                        cACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTgatgat                      >  8:30866/1‑136 (MQ=255)                                                                                                             gACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCg                  <  7:206973/135‑1 (MQ=255)                                                                                                             gACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCg                  <  6:119470/135‑1 (MQ=255)                                                                                                              aCCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGt                <  6:141970/136‑1 (MQ=255)                                                                                                                cTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCCCGGATGGGCAACTGAGCCTGGCGGCCACCGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGc              >  8:269991/1‑136 (MQ=255)                                                                                                                     cAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGcc         >  5:22074/1‑136 (MQ=255)                                                                                                                      aaCCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGcc         >  5:150909/1‑135 (MQ=255)                                                                                                                      aaCCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCACAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGcc         >  6:23772/1‑135 (MQ=255)                                                                                                                       aCCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCCCCCCGGATGGGCAACTGCGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGGTCGACGCTGATGATTCCGGTGCAGGCCCa       <  2:293886/136‑1 (MQ=255)                                                                                                                       aCCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGCCCa       <  6:267010/136‑1 (MQ=255)                                                                                                                       aCCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGCCCa       <  6:195166/136‑1 (MQ=255)                                                                                                                       aCCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGCCCa       <  5:273211/136‑1 (MQ=255)                                                                                                                            tCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGCCCagggag  <  3:3354/136‑1 (MQ=255)                                                                                                                                    |                                                                                                                                GAGCACTTCGGCATGGCCGAAGGCAAGACCGTGGTCGCCGCGCCGTTGATTGCCGAGCTTTCGGCCCCGCGCTTCCTGGCCGGTGGCGACCGTACCAGCCTGGCACTGGACCTGGCCAACCTGTCCGGCCGCGCCCAGCAACTGAGCGTCGAAATCACCACGGATGGGCAACTGAGCCTGGCGGCCAACGCCGTGCAGAGCGTTAACCTGGCCGAAGGGCAGCGTTCGACGCTGATGATTCCGGTGCAGGCCCAGGGAG  >  ACB143/659140‑659398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑