Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,391,491	G→T	6.2%	G529C (GGC→TGC)	ligH →	10‑formyltetrahydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,391,491	0	G	T	6.2%	198.9 / 3.0	65	G529C (GGC→TGC)	ligH	10‑formyltetrahydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (24/37);  new base T (2/2);  total (26/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

CCGGTCTGCATGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTAACGGGGGG  >  ACB143/5391359‑5391626                                                                                                                                      |                                                                                                                                        ccGGTCTGCATGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTgcg                                                                                                                                         <  5:271641/133‑1 (MQ=255)   ggTCTGCATGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGAc                                                                                                                                    <  8:87385/136‑1 (MQ=255)         cATGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGAcatcat                                                                                                                               <  5:19752/135‑1 (MQ=255)         cATGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGAcatcat                                                                                                                               <  2:222902/135‑1 (MQ=255)          aTGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATgc                                                                                                                             >  8:82379/1‑136 (MQ=255)            ggCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATgcg                                                                                                                            >  4:83847/1‑135 (MQ=255)                  gACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGccc                                                                                                                     <  5:144912/136‑1 (MQ=255)                    cGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCgg                                                                                                                   >  5:136816/1‑136 (MQ=255)                      cAGTGCAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGcc                                                                                                                 >  3:157565/1‑136 (MQ=255)                      cAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGcc                                                                                                                 >  8:34371/1‑136 (MQ=255)                          aCAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGcc                                                                                                             >  6:112734/1‑136 (MQ=255)                           ctgCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGcc                                                                                                             >  3:19882/3‑135 (MQ=255)                           cAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCAACGTGGCGGTCTGCGGCGACATAAATCGCATGCCCGGCCTGcc                                                                                                             >  2:128348/1‑135 (MQ=255)                                 ctcGACCGACCCGGCGCTGCGCTGCGCCCCGACCGACCATGTCGT‑GCGCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGt                                                                                                       <  1:254551/135‑1 (MQ=255)                                 ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGt                                                                                                       <  5:175664/135‑1 (MQ=255)                                 ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGt                                                                                                       <  4:106441/135‑1 (MQ=255)                                 ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTc                                                                                                      >  8:225922/1‑136 (MQ=255)                                 ctcGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTc                                                                                                      >  4:200908/1‑136 (MQ=255)                                    gaccgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTAGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTccc                                                                                                    >  1:95771/1‑135 (MQ=255)                                      ccgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGCCATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTc                                                                                                 >  2:270792/1‑136 (MQ=255)                                      ccgaccCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTc                                                                                                 >  3:284591/1‑136 (MQ=255)                                         accCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGc                                                                                              <  3:273760/136‑1 (MQ=255)                                           ccGGCGCTGCGCGGCGCCCCGACCGACCATGTCGTGGCCCGGGCGCGCCGTGATCCTCTCGGCCGGCGCGGAGTGCATCGTGGCGTGCTGCGCCGCCATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCGCGGcc                                                                                             >  2:135093/1‑135 (MQ=255)                                            cGGCGCTGCGCGGCGCCCCGACCGCCCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCTGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGa                                                                                           >  6:37682/1‑136 (MQ=255)                                            cGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGa                                                                                           >  8:103707/1‑136 (MQ=255)                                            cGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCCTGCGCCACCTGATCCTCTCCGCCGGCGCGGCGTTGATCGTGGCGGTCTGCTGCGGCATCATGCGCCAGCCCCGCCTGCCCAAGGGCCCCACGGCacc                                                                                           >  6:41055/1‑134 (MQ=255)                                                       ggcgCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCCGACTTCATCAAGc                                                                                >  4:219759/1‑136 (MQ=255)                                                       ggcgCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGc                                                                                >  3:190904/1‑136 (MQ=255)                                                            cccGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgac                                                                           <  6:270309/136‑1 (MQ=255)                                                             ccgaccgaCCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTcgacg                                                                          <  7:143700/136‑1 (MQ=255)                                                                  cgaccATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAg                                                                     <  7:34486/136‑1 (MQ=255)                                                                   gaccATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAgg                                                                    <  5:81872/136‑1 (MQ=255)                                                                    accATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAggg                                                                   <  5:69409/136‑1 (MQ=255)                                                                    accATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAggg                                                                   <  3:130127/136‑1 (MQ=255)                                                                          tCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCTGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGa                                                              <  5:37682/135‑1 (MQ=255)                                                                          tCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCTGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGa                                                              <  5:41055/135‑1 (MQ=255)                                                                          tCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGa                                                              <  4:288587/135‑1 (MQ=255)                                                                          tCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGa                                                              <  8:207462/135‑1 (MQ=255)                                                                          tCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGa                                                              <  7:103707/135‑1 (MQ=255)                                                                               gCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTTATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                         <  6:191374/136‑1 (MQ=255)                                                                               gCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                         <  6:180746/136‑1 (MQ=255)                                                                                cccGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                         <  5:47624/135‑1 (MQ=255)                                                                                cccGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                         <  3:243911/135‑1 (MQ=255)                                                                                cccGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAg                                                         <  4:256465/135‑1 (MQ=255)                                                                                 caggtcgcgACTTGATCCTCTCGTCCGGCGCGGAGTGCATCGGGGCGGTCTGGGGCGACATGCTGCGCTTGCCCGGCGTGACCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAggg                                                       <  2:227120/131‑1 (MQ=255)                                                                                   gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGCCTTCATCAAGCTCGCCGAGCAGGGCCAGATCCCGGGcc                                                     >  2:289188/1‑136 (MQ=255)                                                                                   gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                     >  8:230461/1‑136 (MQ=255)                                                                                   gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                     >  1:110646/1‑136 (MQ=255)                                                                                   gTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCCGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGcc                                                     >  5:261796/1‑136 (MQ=255)                                                                                      cgcgACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGt                                                  <  4:261516/136‑1 (MQ=255)                                                                                       gcgACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTgcg                                                                                                                                         >  5:73412/1‑48 (MQ=255)                                                                                       gcgACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGtt                                                 >  4:215070/1‑136 (MQ=255)                                                                                       gcgACATGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTgcg                                                                                                                                         <  6:73412/48‑1 (MQ=255)                                                                                            gTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAg                                            <  7:82379/136‑1 (MQ=255)                                                                                            gTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAg                                            <  8:110490/136‑1 (MQ=255)                                                                                              gATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGcc                                          >  6:28654/1‑136 (MQ=255)                                                                                                         ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                               <  1:80899/136‑1 (MQ=255)                                                                                                         ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                               <  5:51891/136‑1 (MQ=255)                                                                                                         ccggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATggg                               <  4:147677/136‑1 (MQ=255)                                                                                                          cggcGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGACTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGa                              >  5:48239/1‑136 (MQ=255)                                                                                                               cgGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAAc                         <  5:285443/136‑1 (MQ=255)                                                                                                                  aGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAAt                      <  3:200908/136‑1 (MQ=255)                                                                                                                   gTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATa                     <  3:117109/136‑1 (MQ=255)                                                                                                                    ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                    <  3:108756/136‑1 (MQ=255)                                                                                                                    ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                    <  2:28170/136‑1 (MQ=255)                                                                                                                    ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                    <  7:262060/136‑1 (MQ=255)                                                                                                                    ttCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATaa                    <  4:116599/136‑1 (MQ=255)                                                                                                                       aTCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAgg                  <  4:157565/135‑1 (MQ=255)                                                                                                                                      ggcgACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTAACgggggg  >  4:285945/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                        CCGGTCTGCATGGCCAAGACGCAGTACAGCTTCTCGACCGACCCGGCGCTGCGCGGCGCCCCGACCGACCATGTCGT‑GCCCGTGCGCGACGTGATCCTCTCGGCCGGCGCGGAGTTCATCGTGGCGGTCTGCGGCGACATCATGCGCATGCCCGGCCTGCCCAAGGTCCCCTCGGCAGACTTCATCAAGCTCGACGAGCAGGGCCAGATCCAGGGCCTGTTCTGAGCCCCGGCCATGGGACGAACAATAAGGCCTCCCTAACGGGGGG  >  ACB143/5391359‑5391626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑