| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,507,078 | A→C | 44.5% | E31A (GAG→GCG) | CJ019_05179 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,507,078 | 0 | A | C | 44.5% | 6.3 / 2.5 | 22 | E31A (GAG→GCG) | CJ019_05179 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/5); new base C (3/7); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCT > ACB143/5506943‑5507204 | gCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGa > 6:7643/1‑136 (MQ=255) tCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCgcgc > 6:211729/1‑136 (MQ=255) tCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGc > 3:70558/1‑136 (MQ=255) cgcgAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCCggcg > 7:9388/1‑135 (MQ=255) gctaataaTCCAACACCTATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAACCCCCCTGGCAACCCACGCCAGCGAGGGCTAATTCTTTCTCACCGCCAACCCCGCCGAGCAggccg < 7:46444/130‑1 (MQ=255) gccaataaTCCAACACCGATTTCCCGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCAAATCCCCCTGGCAACCCACGCCAGCGAGGGCTAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggccg < 2:263920/130‑1 (MQ=255) gccaagaaTCCAACACCGATTTCCAGGATTCCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCTAATTCTTTCTCACCGCCAAGCCCGCCGAGCAggccg < 5:182931/133‑1 (MQ=255) taaTCCAACACCTATTTCCAGGATTGCCCGAATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAACCCCGCCGAGCAGGCCGGCCTg < 7:42036/135‑1 (MQ=255) tCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCACGCCCGCCTCCTCCAATACGGGgcag > 6:207988/1‑136 (MQ=255) tCCAGGATTGCCCGCATGAACAGACTCCTGCTCCCCCTCCTGCTGCAAATCCCCCTCCCAACCCCCGCCAGCGCGGGCTAATTCTTTCTCACCGCCACGCCCGCCGCGCCGGCCGGCCTGCTCGAAGGCTGGgca < 8:9388/135‑1 (MQ=255) ccccggATCGCCCGCATGCTCAGACTCCTGCCCACCCTCCTGCTGAAAACCCCCCTGCCAACCCACCCCCGCGCGGGCTAATTCTTTCCCCCCGCCACCCCCGCCGCGCCGGCCGGCCGGCTCGAAGGCTGGgca < 4:221371/131‑1 (MQ=255) gCATGCTCAGACTCCTGCTCACCCTCCTGCTGATAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCCGCCCGCCCTCGGCAAGGCTGGCCAGCCCAACCCCATGc > 2:247684/1‑136 (MQ=255) ctcctGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCCGCCAGGTAGAACGGAGGGCCGCACAAACCCCAGCCGCCCGCCAGCCCCTGCTggca > 1:222963/1‑133 (MQ=255) aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGGCGGCCGGCCGGCGCGGCGGGGGGGCGGCACCACCCCCGGCCCCGCGCCGGCCGCGGCGGGGCAACCTGCGCCaac > 3:136054/1‑135 (MQ=255) aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGACGGCTGGGCCGCCCACCCCCCTGCCGCCCGCCGGCCGCGGCGGGGAAACCTGCGcccac > 4:175776/1‑133 (MQ=255) aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCCGCCCGCGCGCAGGCTGGGGCGCACCCCCCCAAGCCCCGCGCCGGCCGCGGGTGGGAAACCAGCGCCaac > 1:217044/1‑135 (MQ=255) cccccCGCCCGCGAGGGCTAATTCTTTCCCCCCCCCCCCCCCCCCCCGCCGGCCGGCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGcca < 1:48791/135‑1 (MQ=255) ccGCCAGCGCGGGCTATTTTTTTCCCCCCCCCACGCCCGCCGCGCCGGCGGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCaa < 3:64981/135‑1 (MQ=255) gAGGGCTAATTCTTCCCCCCCCCCCCCCCCGCCGCGCCGGCCGGCCGGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAgc < 5:128780/136‑1 (MQ=255) ttctttctCCGCCGCAGGCCCGGTGCGCCGCCCGGCGTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTg < 3:59375/135‑1 (MQ=255) ctttctCACCGCCAAGCCCGCCGAGGCGGCCGGGCGGCGGGAACGGTGGGGCGCACACCCCCCTGCCCCCCGCCCGCCCCGGGGGGAAAACCAGCGCCAACGCCGCCACGCCCCCAAAGAACAGCCCAACGTgggc > 8:206381/1‑136 (MQ=255) ctttctCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGCAGGCTGGGGCGCCCCACCCCCTGCCGCGCCCCTGCCGCGGGGGGCAAACCAGCGCCCACGCCGCCTCGCCCCCCAAGAAAAGCGCCACGTgcgc > 4:234758/1‑136 (MQ=255) cgcccgcccACCCGGCCGCCCGGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCt < 6:3876/135‑1 (MQ=255) cccccGCCGCGCGGGCCGGCGTGCGCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCt < 7:62374/134‑1 (MQ=255) | GCTCACCCGCGAAGAATCCAACACCGATTTCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACAACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCT > ACB143/5506943‑5507204 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |