Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 6,129,757 A→T 26.3% H430Q (CAT→CAA)  copA_4 ← Copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,129,7570AT26.3% 57.5 / 5.2 43H430Q (CAT→CAA) copA_4Copper resistance protein A
Reads supporting (aligned to +/- strand):  ref base A (15/14);  new base T (3/7);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCA  >  ACB143/6129623‑6129884
                                                                                                                                      |                                                                                                                               
ccAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCATCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCAt                                                                                                                                <  3:205066/136‑1 (MQ=255)
 cAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATGTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCACAGCCATTTTGCTGTGTTCGATGCCGGCCAGGCTGCCagg                                                                                                                               >  1:243887/1‑134 (MQ=18)
           gCTATGGTCCATATCGGCCATGAAGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGATGCCGTGGTACATAGCCGCCATTTTGCAGTGCTACATACAGGCCATGCTACCATTGTACATaccc                                                                                                                     >  8:163767/1‑135 (MQ=9)
               tgtgccAGATCGGCCATGCCGGCTTGGACCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGcc                                                                                                                  <  5:208495/131‑1 (MQ=255)
                   ccATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                             <  8:83760/136‑1 (MQ=255)
                    cATATGGGCATTGCGGCGTTGGTCCATCCCAGCCTTCTTGCTGTGTTCCATGGGGGCCATGCCGGCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATTCTGCCATGGTCCATGCCAGCCAtttt                                                                                                             <  7:124286/135‑1 (MQ=255)
                    cATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTg                                                                                                            <  5:98281/136‑1 (MQ=255)
                           gCCTTGCCGCCGTCGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGCTCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGt                                                                                                     <  8:114934/136‑1 (MQ=17)
                              aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCAGACCAGCCATTTTGCAGTGGTCCa                                                                                                  >  1:158739/1‑136 (MQ=255)
                              aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGAGGCCGTGGTCCATCGCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTTCCATGGTCCATCCCCGCCATGTTGCTGTGGTCCa                                                                                                  >  2:177766/1‑136 (MQ=17)
                                 ccgccTTGGTCCAGACCAGCCATCTTGCGGTGGTCCCTGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCAt                                                                                                 <  4:161/134‑1 (MQ=255)
                                   gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                             <  3:271845/120‑1 (MQ=255)
                                   gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                             >  4:271845/1‑120 (MQ=255)
                                   gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCACAGCCATTTTGCTGTGCTCCATCCCGGCCATGCTGCCATGGTCCATCCCAGCAAGATTGCATTGGTCAATTCCg                                                                                             >  8:156333/1‑136 (MQ=17)
                                   gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCACAGCCATTTTGCTGTGATCCATGCCGTCCATGATGCCTTGGTCCATCCCAGCCATATTGAAGTAGTCAATTCCg                                                                                             >  6:46432/1‑136 (MQ=17)
                                        gggccATCCCAGCCATGTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                          <  1:233074/131‑1 (MQ=255)
                                        ggTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                          <  8:29839/134‑1 (MQ=255)
                                            cATCGCATCCATCTTGTTTTGGTCCTTGACTGCCTTGACGCCGTGGTCCATCTCTTCCATTTTGCTGTGTTCCATGTCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGtt                                                                                     <  8:145741/135‑1 (MQ=17)
                                                ccAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCg                                                                                 <  3:259931/135‑1 (MQ=255)
                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGGCATGTTGCCGtc                                                                               >  3:100910/1‑135 (MQ=255)
                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGATGCCATGGTCCATCCCCGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCAGTg                                                                               >  5:68926/1‑136 (MQ=255)
                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGACGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCAGGCCATGATGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCGCTGAGGGGCATGTTggagtg                                                                               >  7:275975/1‑131 (MQ=11)
                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGAAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAGTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTg                                                                               >  8:180170/1‑136 (MQ=255)
                                                       tCTTGCTGTGGTCCATGCCGGCCATGCAGCCGTGGTCCATCACAGCCATTTTGCTGTGATCCATCCCGGCCATGATGCCTTGGTCCATCACCGCCATGTTGCTGTGGTCCATACAGGACATGTTGCGGTGGTCCAt                                                                         >  7:280565/1‑136 (MQ=11)
                                                       tCTTGCTGTGGTCCATGCCGGCCATGAAGCCGTGGTCCATCACAGCCATTTTGCTGTGATCCATGCCGGCCATGATGACATGGTCCATCCCCGCCATTATGCTGTGCTCCATCCCGGAAATGATGCAGTGGTcaa                                                                          >  8:132189/1‑133 (MQ=14)
                                                               ttgTCCATTCGGGCCATGCGGCCTAGTTCGATGACAGCCATTTTGGTGTGTTGAATGTCGGCCATGCTGGCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGcc                                                                  <  4:56190/133‑1 (MQ=11)
                                                               tgGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGcc                                                                  <  5:253120/135‑1 (MQ=255)
                                                                    cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGCTCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATGTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTg                                                            <  6:70088/136‑1 (MQ=255)
                                                                         cGGCCATGCAGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAGTATGCtgtg                                                       >  6:130419/1‑136 (MQ=255)
                                                                               tGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGcc                                                                                                                  >  7:100790/1‑71 (MQ=255)
                                                                               tGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGcc                                                                                                                  <  8:100790/71‑1 (MQ=255)
                                                                                 ccgccgTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                               <  3:288880/136‑1 (MQ=255)
                                                                                        ggTCCATCACTTCCATTTTGCTGTGATCCATTGCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGcc                                          <  7:72724/134‑1 (MQ=11)
                                                                                            cATCTCAGCCATTTTGCTGTGTTCCATGTCGGCCATGCTGCCCTGTTCCATCCCAGCCATTTTGATTTGGTCCATGACGGCCATGATGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTg                                    <  8:250056/136‑1 (MQ=11)
                                                                                            cATCGCAGCCATTTTGCTGTGCTCCATGCCGGCCATGCGGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTg                                    <  6:117800/136‑1 (MQ=255)
                                                                                                       ttttGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCAt                         >  7:27808/1‑136 (MQ=255)
                                                                                                             tgtgATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGACGTGGTCCATCCCAGCCATTTTGCTGTGGTCCAAGCCGGCCATGTTGCCGTGGTCCAGCCCac                    >  5:65888/1‑134 (MQ=255)
                                                                                                                   ccctGCCGGCCATGAGGCCGTGGACCATCCCTGCCATCTTGCTGTGGTCCCTGCCGGCCATGTTGCCGTCGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt             <  3:13816/133‑1 (MQ=11)
                                                                                                                    cctGCCGGCCATGCTGCCCTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGACATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt             <  1:158489/133‑1 (MQ=255)
                                                                                                                    cATTCCGGCCATGCTGCCTTGGTCCATCCCAGCCATCTTGCTTTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt             <  1:110810/135‑1 (MQ=255)
                                                                                                                    cATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGGCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTg            <  3:258927/136‑1 (MQ=255)
                                                                                                                    cATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGATGCCGTGGTCCAGCCCAGCCATTATg            >  5:38776/1‑136 (MQ=255)
                                                                                                                            ccATGCTGCCATGGTCCATCCCAGCCATTTTCCTGTGGTCCATGCCGGACATGTTGCCGTGGTCGATCCCAGCCAGTATCCTGTGGTCCATGCCGGCCTTGTTGCCGTGGTACATCGCAGACTTTTTGCTTTGGTc    >  6:48500/1‑136 (MQ=14)
                                                                                                                              aTGCTGCCATGGTCCATCCCCGCCATTTTGCTGTGGTCCATGCCGGTCATGTTTCCGTGGTCCATCACAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCCGCAATTATGCTGTGGTCCa  >  7:186650/1‑136 (MQ=17)
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CCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCA  >  ACB143/6129623‑6129884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑