| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,390,258 | Δ2 bp | coding (352‑353/1666 nt) | ligH → | 10‑formyltetrahydrofolate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,390,258 | 0 | G | . | 100.0% | 122.4 / NA | 26 | coding (352/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (7/19); total (7/19) | |||||||||||
| * | ACB143 | 5,390,259 | 0 | T | . | 100.0% | 122.4 / NA | 26 | coding (353/1666 nt) | ligH | 10‑formyltetrahydrofolate synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (7/19); total (7/19) | |||||||||||
CCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTC > ACB143/5390136‑5390380 || ccGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCTGCTATGCGCAG‑‑CGTGCCGATggagg < 5:71238/136‑1 (MQ=255) cGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATggagga < 5:257500/136‑1 (MQ=255) tGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAAc < 1:392585/135‑1 (MQ=255) tCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCAcc < 1:183673/135‑1 (MQ=255) tCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCAcc < 1:107957/135‑1 (MQ=255) tCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCAcc < 7:217134/135‑1 (MQ=255) gCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCa < 1:126120/134‑1 (MQ=255) tGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCg < 7:370278/135‑1 (MQ=255) ttCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACCTCACCCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACACCCTGCTcgccgc > 6:318354/1‑136 (MQ=255) gCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTcgccgc < 2:320300/133‑1 (MQ=255) gCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTcgccgc < 7:313415/133‑1 (MQ=255) tCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTg < 3:112386/135‑1 (MQ=255) gCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg < 5:403192/136‑1 (MQ=255) ccccTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg < 7:225987/135‑1 (MQ=255) ccccTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCg < 1:99827/135‑1 (MQ=255) ccTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTTCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGAc < 3:286059/135‑1 (MQ=255) cGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCt > 4:93364/1‑135 (MQ=255) tGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTAc > 4:130093/1‑135 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACcttca < 7:129658/57‑1 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACcttca > 8:129658/1‑57 (MQ=255) ggcggcGCGGCCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTggg > 1:247189/1‑134 (MQ=255) gcggcgcggcCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGc < 3:100058/135‑1 (MQ=255) cgcggcCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGcaa > 3:351600/1‑133 (MQ=255) gcggcCGGCGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGcaacaa < 4:147618/135‑1 (MQ=255) ccggcGGCGGCTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTc > 7:373284/1‑135 (MQ=255) ggcggcTATGCGCAG‑‑CGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTc < 5:229629/136‑1 (MQ=255) || CCGTGGGCCTGGCCGACGGCCTCAACCGCATCGGCAAGAAGACCGTGGCGGCGCTGCGCGAGCCTTCGCTCGGCCCCTGTTTCGGCGTGAAGGGCGGCGCGGCCGGCGGCGGCTATGCGCAGGTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTC > ACB143/5390136‑5390380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |