Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 5,760,598 (A)6→5 coding (781/834 nt) CJ019_05400 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,760,5930A.95.8% 211.4 / 1.8 49coding (776/834 nt)CJ019_05400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (34/12);  minor base T (0/2);  total (34/15)
Fisher's exact test for biased strand distribution p-value = 8.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB143/5760461‑5760721
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aGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaa                                                                                                                                 >  7:370906/1‑134 (MQ=255)
          gcgcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATAGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGATGAAGAGGCACCCAACAAGGACCTGCTGGCGCAGCACGACGCGG‑AAAACCGCCGCcag                                                                                                                    >  5:306441/1‑136 (MQ=255)
                       cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTg                                                                                                        >  1:37654/1‑135 (MQ=255)
                        aGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTgg                                                                                                       >  4:394897/1‑135 (MQ=255)
                              aCCTGGCCAAGGTCTTGAAGACCATCGAGCAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGCCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                 <  1:307748/135‑1 (MQ=255)
                               ccTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccgcc                                                                                               >  5:365387/1‑136 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAACCCCTCGCCCGCCAGGCCCGAGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGGAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  1:356334/1‑135 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  4:303755/1‑135 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  4:241050/1‑135 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  8:234181/1‑135 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg                                                                                              >  7:317798/1‑135 (MQ=255)
                                 tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCAGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                             >  5:381637/1‑136 (MQ=255)
                                      aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                        >  6:281805/1‑136 (MQ=255)
                                      aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACACCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGcc                                                                                         >  2:113232/1‑135 (MQ=255)
                                          tCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                                     <  3:184555/135‑1 (MQ=255)
                                           cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc                                                                                   >  5:312065/1‑136 (MQ=255)
                                                aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              >  6:220396/1‑136 (MQ=255)
                                                aGACCATCGAGCAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  1:76163/1‑135 (MQ=255)
                                                 gACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  2:135840/135‑1 (MQ=255)
                                                     aTCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGt                                                                          <  2:310790/135‑1 (MQ=255)
                                                       cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  7:282660/1‑135 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCCGGCCCGTGAAGCTGAAGAGGCACTCAAGAAGGCCCTGCTGGCGAAGCTGGAAGCGG‑AAAACCTCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTCCCAGCGCCCGGGCGCGTgcgc                                                                      >  1:361508/1‑135 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTTCAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      <  3:93236/135‑1 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTgcgc                                                                      >  8:323763/1‑135 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGcc                                                                     >  6:368299/1‑136 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGcc                                                                     >  6:98196/1‑136 (MQ=255)
                                                         aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  7:227712/1‑135 (MQ=255)
                                                             aaCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  5:335287/135‑1 (MQ=255)
                                                                 cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCagag                                                              >  7:141164/1‑135 (MQ=255)
                                                                 cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                             >  6:216906/1‑136 (MQ=255)
                                                                     cccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAg                                                          >  7:15182/1‑135 (MQ=255)
                                                                       cgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGt                                                         >  4:6418/1‑134 (MQ=255)
                                                                            ggCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAcc                                                   >  3:360668/1‑135 (MQ=255)
                                                                                    aaGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  6:365387/136‑1 (MQ=255)
                                                                                     aGCTTATGAGGCACGAAAGAAGGCCCTGCTGGCGCAGCCGTACGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  3:323748/135‑1 (MQ=255)
                                                                                     aGCTGACCAGGCACGCAAGAAGGCCCTGCGGGCGCAGCAGGAAGC‑GCAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  7:290724/135‑1 (MQ=255)
                                                                                     aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          >  1:142822/1‑135 (MQ=255)
                                                                                     aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          >  3:26779/1‑135 (MQ=255)
                                                                                           cccggaaCGAAAGAAGGCCCTGCTGGCGCAGCAGCATGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGccc                                     <  2:416424/128‑1 (MQ=255)
                                                                                            gagGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGt                                   >  3:76936/1‑135 (MQ=255)
                                                                                                cACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                              <  5:172078/136‑1 (MQ=255)
                                                                                                    cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                           >  8:318971/1‑135 (MQ=255)
                                                                                                    cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                          >  6:78701/1‑136 (MQ=255)
                                                                                                     aagaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                          >  3:14542/1‑135 (MQ=255)
                                                                                                         cgggccTGCTGGCGCTGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                      <  2:76163/131‑1 (MQ=255)
                                                                                                          ggCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGcc                     <  4:14542/135‑1 (MQ=255)
                                                                                                                gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt               >  4:265946/1‑135 (MQ=255)
                                                                                                                    gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc           >  8:197803/1‑135 (MQ=255)
                                                                                                                           agGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg    <  7:197803/135‑1 (MQ=255)
                                                                                                                             gAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  3:303755/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB143/5760461‑5760721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑