New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 2155685 =47 (0.710)61 (0.920) 32/260 0.7 53.3% coding (301/369 nt) tmk_1 Thymidylate kinase
?ACB143 = 2220562 60 (0.910)coding (36/369 nt) tmk_2 Thymidylate kinase

GAAACAGCTTTGAGCAATGCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB143/2155815‑2155685
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCAAAAATGC  <  ACB143/2220562‑2220438
                                                                                                                                                                                                                                                                
GAAACAGCTTTGAGCAATGCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGC                                                                                                                            <  8:199308/134‑1
                ATGCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTT                                                                                                           <  3:93508/135‑1
                 TGCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         <  5:263352/136‑1
                 TGCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTG                                                                                                          <  7:266178/135‑1
                  tcaaccCTGCCATGATCTAAGCTGAATCCCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         <  2:385033/129‑1
                  GCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         <  1:72634/135‑1
                  GCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         <  2:313325/135‑1
                  GCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         <  8:345072/135‑1
                    TACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTGTTGTATTCGTTCTTGAG                                                                                                        >  7:367165/1‑134
                    TACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAG                                                                                                        >  8:362354/1‑134
                    TACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTG                                                                                                                      <  6:255542/120‑1
                    TACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTG                                                                                                                      >  5:255542/1‑120
                       GCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAA                                                                                                    <  3:243566/135‑1
                         TGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATG                                                                                                  >  7:82261/1‑135
                         TGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATG                                                                                                  >  7:327156/1‑135
                         TGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATG                                                                                                  >  6:186557/1‑135
                         TGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAAT                                                                                                   >  1:297093/1‑134
                         TGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTCAGCAGTAAAAGAATATAGGGCGTATTGTATTCGTTCTTGAGTAAATGT                                                                                                 >  6:41613/1‑136
                           CCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTGTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAA                                                                                               <  6:157039/136‑1
                            CATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACGATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAA                                                                                               <  1:68451/135‑1
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGCCCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTAGTTCTTGATTAAATGTAAAC                                                                                             >  7:330299/1‑135
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAAGCTAGCTCACCATCAAATTTCTCTTCATGTTTACGTTGAGCATTATAAGATAATAGGGCGTCTTGTATTAGTTCTTGAGTAAATGTAAACA                                                                                            >  6:285976/1‑136
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGCGCATTAAAAGAATATAGGGCGTCTTGTAGTCGTTCTTGAGTAAATGTAAAC                                                                                             >  7:335503/1‑135
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGTGCGTATTGTATTAGTTCTTGAGTAAATGTAAA                                                                                              >  7:289259/1‑134
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACA                                                                                            <  6:78585/136‑1
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACA                                                                                            <  6:279427/136‑1
                              TGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTGGTATTCGTTGTTGATTAAATGTAAAC                                                                                             >  8:366642/1‑135
                               GATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACA                                                                                            <  7:175393/135‑1
                                     CGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATA                                                                                          <  6:360976/131‑1
                                      GCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATTTAAACATAGGCCTA                                                                                    >  6:558/1‑136
                                      GCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATA                                                                                          <  2:297093/130‑1
                                      GCTGAATAGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATA                                                                                          <  4:141014/130‑1
                                        TGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTAT                                                                                   <  2:297050/135‑1
                                        TGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         >  5:87146/1‑113
                                        TGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGA                                                                                                         <  6:87146/113‑1
                                        TGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTGCTTGAGTAAATGTAAACATAGGCCTAT                                                                                   >  4:316561/1‑135
                                            TCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATA                                                                               <  3:151769/135‑1
                                              GCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACG                                                                             >  4:239913/1‑135
                                                  GATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTgc                                                                         >  2:22956/1‑133
                                                       TACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTAC                                                                     <  7:292165/134‑1
                                                                 GGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAA                                                         <  5:102498/136‑1
                                                                 GGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTA                                                           <  3:2108/134‑1
                                                                  GCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAA                                                         <  7:326003/135‑1
                                                                  GCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAA                                                         <  7:38432/135‑1
                                                                  GCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAA                                                         <  4:255493/135‑1
                                                                     ACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACAT                                                      <  7:333295/135‑1
                                                                         AACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGT                                                  >  4:122215/1‑135
                                                                         AACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAG                                                   >  2:217267/1‑134
                                                                           CCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCT                                               <  5:45128/136‑1
                                                                            CTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCGTTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCT                                               <  4:250558/135‑1
                                                                            CTAGCTCACCATCAAATTTCTCATCATGTTTACCTTGATCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCT                                               <  8:190876/135‑1
                                                                                  CACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCC                                         >  3:395890/1‑135
                                                                                    CCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCT                                       <  8:43259/135‑1
                                                                                        CAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAA                                   >  1:65104/1‑135
                                                                                                             CTTGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTACCTTACTAAGAATGTAAACATGAGTTCTACATCACTCTAACCACCGAAATTCGGGGCCTGGA             >  5:36466/1‑136
                                                                                                               TGAGCATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAG            >  3:229094/1‑135
                                                                                                                   CATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCA         >  8:15617/1‑134
                                                                                                                    ATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCAAA       >  6:348190/1‑135
                                                                                                                    ATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCAA        >  4:57415/1‑134
                                                                                                                    ATTAAAAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCAA        >  3:398244/1‑134
                                                                                                                         AAGAATATAGGGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCAAAAATGC  >  6:147396/1‑135
                                                                                                                                                                                                                                                                
GAAACAGCTTTGAGCAATGCTACGCTGCCATGATCTACGCTGAATCGCCTGATTTTACTGGCTTAGGCGACCAAACCTAGCTCACCATCAAATTTCTCTTCATGTTTACCTTGAGCATTAAAAGAATATAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB143/2155815‑2155685
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGCGTCTTGTATTCGTTCTTGAGTAAATGTAAACATAGGCCTATTATACGTTCCTTACTAAGAATGTAAACATGAGTTCTACATCCCTCTAACCCCCGAAATTCGGGGCCTGGAGCCAAAAATGC  <  ACB143/2220562‑2220438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.