New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 569671885 (1.290)3 (0.050) 3/260 7.4 3.4% coding (3/1680 nt) phaC_1 Poly(3‑hydroxyalkanoate) polymerase subunitPhaC
?ACB143 5702993 = 83 (1.260)coding (3/420 nt) CJ019_05350 hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCCTTATCACTGGTCACCCGAGTAGCAGTACCGGGCTCAGGGCTGTGCACCCGCCACAGCAACCGGTACTCGTCTCAGGACAACGGAGCGTCGTAGATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5696619‑5696718
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAA  >  ACB143/5702993‑5703079
                                                                                                                                                                                           
TTCCTTATCACTGGTCACCCGAGTAGCAGTACCGGGCTCAGGGCTGTGCACCCGCCACAGCAACCGGTACTCGTCTCAGGACAACGGAGCGTCGTAGATGCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAA                                                     <  6:370595/136‑1
                                                        ACAGCAACCGGTACTCGTCTCAGGACAACGGAGCGTCGTAGATGCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAA  <  1:233538/131‑1
                                                        ACAGCAACCGGTACTCGTCTCAGGACAACGGAGCGTCGTAGATGCATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAA  >  2:233538/1‑131
                                                                                                                                                                                           
TTCCTTATCACTGGTCACCCGAGTAGCAGTACCGGGCTCAGGGCTGTGCACCCGCCACAGCAACCGGTACTCGTCTCAGGACAACGGAGCGTCGTAGATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5696619‑5696718
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATGCTGTGTACCTCATGCTCATGAGTGGTGGAAAACCGCCCATGGAGGCTGGGCTTGAGCATTAAGGTAGGGAAGAAAATTAGAAA  >  ACB143/5702993‑5703079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.