Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 3,273,878 | 0 | C | A | 34.4% | 80.3 / 29.8 | 61 | D40E (GAC→GAA) | fabG_5 | 3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG |
Reads supporting (aligned to +/- strand): ref base C (28/12); new base A (0/21); total (28/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.77e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGG > ACB143/3273750‑3274011 | gAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACt > 2:306678/1‑134 (MQ=255) caggcaTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAg < 1:51236/134‑1 (MQ=255) acaaAACGTCGGGCTGGTGATCGGGGCGGGCGACGCCACTGGGGGCGAGATCGCCAAGCGTTTTGCGCGTGTGGGCTATATTGCCTGCGTAACCGGTCGCCAGGCCGAAAAACTGCAGCCGCTGCTGGAGTAAAt < 1:255278/135‑1 (MQ=255) aaaGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATacac > 1:290061/1‑135 (MQ=255) aaaGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACAcg > 6:304610/1‑136 (MQ=255) cGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACAcgccgc > 1:375326/1‑135 (MQ=255) gCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAAATGCAGCCGATGCTGGAGGAAATACACGCCGCTgg > 2:229579/1‑135 (MQ=255) tgggcaTCGGGGCGGGCGACGCCACGGGCGGCGAGATCGCCAAGCGTTTTGCGCGGGAGGGCTATATTCCCTGCTAAACCCGGCCCCAGGCCGACAAACTGCAGCCGCGGCTGGAGGAAATCCACGCCGCTggcg < 7:115558/130‑1 (MQ=255) tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcg > 1:292404/1‑135 (MQ=255) tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcg > 1:386573/1‑135 (MQ=255) tGGTGAGCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGGGAGGGCTATATTGCCTGCGTAACCCGGCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcg < 8:381016/135‑1 (MQ=255) gcGGGGCGGGCGACGCCACGGGGGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGAACCCCGTCGCCAGGCCTACAAACTGCCGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGc < 5:343208/135‑1 (MQ=255) ggggCGGGCCAGGCCAGTGGCGGCGATAGCGCCAAGCGTTGGGCGGGTTAGGGCTATATTGCGGGCGAACCCCGCCGCCAGGCCTAAAAACTCCCGCCGCGGCTGGAGGAAAAACACGCCGCTGGCGGCCAGGcc < 8:343129/135‑1 (MQ=255) gggggcgACGCCCCTGGGGGCGAGATCGCCACGGGTTTTGCGGGTGAGGGATATATTGCTTGCGTACCCCGCCCCCCGGCCTAAAAACTCCAGCCGCTGCTGGAGTAAAACCACGCCGCTGGCGGCCAGGCCTAc < 8:330062/133‑1 (MQ=255) cgACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGttt > 4:195073/1‑135 (MQ=255) cACTGGCGGCGAGATCGCCAAGCGTTTGGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGAAAAACTGCAGCCGCTGCTGTAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGctct < 5:195165/136‑1 (MQ=255) cggcgAGAGCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCGTGCGTAACCCGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgc < 2:134689/135‑1 (MQ=255) ctagaTCGCCAAGCGTTTTGCGGGTGTGGGCTATATTGCCTGGGAAACCCGGCGCCAGGCCTAAAAAGTGCCGCCGCTGCTGGATTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcgcg < 4:348642/133‑1 (MQ=255) gTTTTGCGCGTGAGGGATATATTGCCGGCGAACCCCGCCGCCCGGCCGAAAAACTGCACCCGCGGCTGGAGTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGt < 5:359308/136‑1 (MQ=255) ttttGCGCGTGAGGTCTATTTGCCCTGCTTAACCCGTCGCCAGGCCGACAAACGGCCGCCGCTGCGGGATTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGt < 8:154727/135‑1 (MQ=255) ggggggTGAGGGCTATATTGCCTGCGAACCCCGCCGCCCGGCCCAAAAACTGCCGCCGCTGCTGTTGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTgg < 1:394773/129‑1 (MQ=255) gcgcGGGAGGGATATATTGCCTGCGTAACCCGTCGCCAGGCCGAAAAACTGCCGCCGGTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc < 5:130902/135‑1 (MQ=255) cgcgTGAGGTCTTTTTTGCCTGCGTAACCCGTCGCCAGGCCTAAAAACTGCTGCCTGTGCTGGAGTAATAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc < 7:263895/134‑1 (MQ=255) cgcgTGAGGGCTATATTGCCTGCGTAACCGGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGAGGAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc < 7:41642/134‑1 (MQ=255) gcgTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGAGCGCAAGGAAGAGGAGGTGGCCGa > 7:339489/1‑135 (MQ=255) gggAGGGATATATGGCGTGCGAACCCGGTCGCCCGGCGCAAAAACGGCACCCGCGGGTGGAGCAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt < 6:110162/134‑1 (MQ=255) ttggttcTATTTTGCGTGCTTAACCCGGCGCCCGCCCCAAAAACGCCCGCCGCGGCTGGAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt < 4:72250/129‑1 (MQ=255) atTGCCTGCTTAACCCGTCGCCAGGCCTACAAACTGCCGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt < 4:164266/135‑1 (MQ=255) atTGCCTGCGTAACCCGGCGCCAGGCCGACAAACTGCAGCCGGTGCTGTAGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACt < 3:51692/135‑1 (MQ=255) tGCCTGCGTAACCCGTCGCTAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTAt > 2:261777/1‑135 (MQ=255) gCCTGCGTAACCCGTCGCCTGGCCGACAAACTGCAGCGGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATc < 5:177626/135‑1 (MQ=255) ccggCGTAACCGGGCGCGAGGCCTACAAACTGCAGCGGGTTGTGGTGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 6:325389/133‑1 (MQ=255) ccTGCTTAACCCGTCGCCCGGCCCAAAAACTGCCGCGGGTTCGGTAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 5:31230/136‑1 (MQ=255) cggCGTAACCCGGCGCCAGGCCGAAAAACTGCAGCGGCTGGGGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 7:120050/133‑1 (MQ=255) cggCGAAACCCGGCGCCAGGCCTAAAAAGGGCCGCCGCTGCTGGAGCAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 7:306021/133‑1 (MQ=255) cTGCGTACCCCGTCGCCAGGCGGAAAAACTGCCGCCGGGGCTGGTGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 3:115149/135‑1 (MQ=255) cTGCGTAACCCGTCGCCAGGCCGACAAACTGCACCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 7:373304/135‑1 (MQ=255) cTGCGTAACCCGCCCCCAGGCCGAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa < 7:208440/135‑1 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGCTGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 1:308556/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 8:146894/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 2:339535/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 2:73762/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 3:232919/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 2:207299/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 3:71715/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 2:20030/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGc > 7:199542/1‑135 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCg > 5:260004/1‑136 (MQ=255) gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCg > 6:26041/1‑136 (MQ=255) cGTACCCCTGCGCCGGGCCGAAAATCTGCCGCCGGTGCTGGATGAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGTGGAGGTGGCCGAACTGGTAGAGACTATCGAg < 3:56343/133‑1 (MQ=255) ttAACCCGTCGCCTGGCCTAAAAACTGCAGCGGGTGCTGGATTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt < 3:42096/134‑1 (MQ=255) gTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt > 6:234652/1‑135 (MQ=255) gTAACCCGGCGCCAGGCCGACAAACTGCAGCCGCTGGTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGt < 2:75988/135‑1 (MQ=255) aCCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATTGAGCGTGat > 8:138354/1‑135 (MQ=255) ccAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTcc < 2:2087/135‑1 (MQ=255) cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCg > 4:137663/1‑135 (MQ=255) cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCg > 3:88643/1‑135 (MQ=255) cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCg > 3:164466/1‑135 (MQ=255) cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGCTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCg > 4:66030/1‑135 (MQ=255) ccGAAAACCTGCAGCCGCGGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTg < 1:255316/135‑1 (MQ=255) gACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAgg > 5:251628/1‑136 (MQ=255) | GAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAAGCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGATTGAGG > ACB143/3273750‑3274011 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |