Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,161,3250GT46.7% 24.8 / 15.5 45intergenic (‑127/‑377)CJ019_04854/puuA_3hypothetical protein/Gamma‑glutamylputrescine synthetase PuuA
Reads supporting (aligned to +/- strand):  ref base G (18/6);  new base T (0/21);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 7.92e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGTCATGGGGCTTTTTAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTAT  >  ACB143/5161192‑5161444
                                                                                                                                      |                                                                                                                       
cagtcaTGGGGCTTTTTAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCATTTGTagcgg                                                                                                                        >  1:162295/1‑134 (MQ=255)
      tGGGGGTTTTTTGTGATGCTGGGGAGCCTGCTTTTTCATGATCGGAATTCGGAAAGTCGGGCGATTGTCGCTGATTTGCGGATTCTCTGCTGCCTGCACGCTCCGTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGc                                                                                                                  <  8:371653/135‑1 (MQ=255)
           cTTTTTAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCaa                                                                                                             >  7:92129/1‑135 (MQ=255)
           cTTTTTAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCaa                                                                                                             >  4:124147/1‑135 (MQ=255)
           cTTTTTAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTAGCGATAGGGCCGCaa                                                                                                             >  2:337965/1‑135 (MQ=255)
                tAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCg                                                                                                        >  1:115583/1‑135 (MQ=255)
                tAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGAAAGGCCg                                                                                                        >  2:19589/1‑135 (MQ=255)
                tAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTAGCGCTAGGGCCGCAAGGccc                                                                                                        >  7:330072/1‑134 (MQ=255)
                tAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCGGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCg                                                                                                        >  3:214294/1‑135 (MQ=255)
                       gCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACt                                                                                                >  6:169883/1‑136 (MQ=255)
                           gTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATGGGCGCAGATTTGCGGATTCTCTGCGGCCGGCACGCTCCCTGTGG‑AAGCGGCCTTGTGGCGCGATAGGGCCGCAAGGCCGCTTCCACTTTg                                                                                             <  1:337965/135‑1 (MQ=255)
                              cgCCTGGTTTTTCATGATCGGAATTCGTAAAGGCGGGCGATTGTCGCAGATTTGCGTATTCTCTGCTGCCTGCCCGCGCCGTGTGG‑ACGCGGCCTTGTGGCGCTATAGGGCCGAAAGCCCGCTTCCACTTTGAcc                                                                                          <  4:313086/134‑1 (MQ=255)
                              aGCCTGTTTTTTCATGAGCGGCATTGGTAACGTGGGGCGTTTGTCGCATTTTTGCGTTTTCTCTGCGGCCTGCACGCCCCCTGGGT‑AAGGGGCTTTGTGGCGCTATTGGCCGGAATGCCCGCTTCCACTTTGACCg                                                                                         <  6:128191/136‑1 (MQ=255)
                                   gCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑CAGCGGCCTTGTGTAGCGATAGGGCCGCAAGGCCGCTTGCACTTTGACCgtggt                                                                                     >  1:167356/1‑135 (MQ=255)
                                       tttCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACGTTGACCGTGGTGCttt                                                                                >  6:172326/1‑136 (MQ=255)
                                       tttCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAAGCCGCTTACACGTTTACCGTGGTGCtt                                                                                 >  5:246399/1‑135 (MQ=255)
                                                 ggCATGCGGAACGGGGGGCGATTGTCGCATTTTTGCGGATTCTCTGCTGCCTCCACGCCCCCTGTGC‑AAGCGGCCTTGTGGCGCTATAGGGCCGCATGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAAt                                                                      <  6:397837/136‑1 (MQ=255)
                                                           aaGTCTGGCGATTGGCGCAGATTTGCGGAGTCTCTGCTGCCGGCACGCTCCCTGTGG‑ACGCGGCTTTGTGTCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  6:303693/136‑1 (MQ=255)
                                                           aaGTCGGGCGTTTGTCGCTTATTTGCGGTTTCTCTGCTGCGGGCACGCTCCCTGTGG‑AAGCGGCGTTGTGTCGGTTTAGGGCCGCATGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  5:313647/136‑1 (MQ=255)
                                                            cgTCGGGCGTTTGTCGCATATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGGCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  1:383487/134‑1 (MQ=255)
                                                            atccGGGCTATTTGCGCAGATTTGCGGAGACTCTGCGGCCTGCACGCTCCCTGTGG‑ACGCGGCCTTGTGTCGCGATAGGGCCGAAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  1:341465/132‑1 (MQ=255)
                                                            aggcGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  1:325645/132‑1 (MQ=255)
                                                            aGTGGGGCTATTGTCGCAGATTTGCGGTTGCTCTGCGGCCTGCACGCGCCCTGTGG‑ACGCGGCGTGGTGGCGCGATAGGGCCGAAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  1:26287/135‑1 (MQ=255)
                                                            aGTCGGGCTATTGTCGCAGATTTGCTTATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGTCCTTGTGTCGCTATAGGGCCGAAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  7:280034/135‑1 (MQ=255)
                                                            aGTCGGGCGATTGTCGCTGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCaa                                                            <  1:53801/135‑1 (MQ=255)
                                                                gggCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATATGGCCGCAAGGCCGCATACACTTTGACAGTGGTGCTTTCAACAAGAATGTTTAGCCAAGTTCg                                                       >  5:117526/1‑136 (MQ=255)
                                                                         tCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCATGTGTCGCGATAGGGCCGCAAGGCCGCTTACACGTTGCCCGTGGTGGGTTCAACACGGATTGTTATCCAAAGGGGATGTaaat                                               >  1:203241/1‑134 (MQ=255)
                                                                                 ttGCGTATTCTGTGCGGCCTGAACGGCCCCTGTGG‑AAGCGGCCTTGTGTCGCTATAGGGCCGCATGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCAt                                       <  7:322818/135‑1 (MQ=255)
                                                                                 ttGCGTATTCTCTGCTGCCTGCACGCTCCGTGTGG‑AAGCGGCCTTGTGTCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCAt                                       <  5:201393/135‑1 (MQ=255)
                                                                                  tGCGGTTGCTCTGCTGCCTGCACGCGCCCTGTGT‑AAGGGCCGTTTTGTCGCTATAGGCCCGAATGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCAt                                       <  1:345973/134‑1 (MQ=255)
                                                                                  tGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCAt                                       <  1:39808/134‑1 (MQ=255)
                                                                                   gCGTATTCTCTGCTCCCGGCACGCTCCCTGTGG‑ATGGGGCCTTGTGTCGCTATAGGGCCGAATGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCa                                    <  6:140065/136‑1 (MQ=255)
                                                                                     ggATTCTCTGCTGCCTGAACGCTCCCTGTGG‑ACGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCAc                                   <  1:237601/135‑1 (MQ=255)
                                                                                      gATTCTCTGCTGCCTGCACGAGCCCTGTGG‑AAGCGGCATTGTGTCGCTATAGGGCCGAAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACt                                  <  7:74462/135‑1 (MQ=255)
                                                                                             tgctgcCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTa                            <  5:370310/134‑1 (MQ=255)
                                                                                                     gccggCTCCCTGTGT‑ACGGGGCGTTTTGTCGCTATTGGGCGGAATGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCtt                   <  3:160083/131‑1 (MQ=255)
                                                                                                     gcACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTa                  >  5:379665/1‑136 (MQ=255)
                                                                                                     gcACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACGTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGGTTTAATCAACCt                    >  1:187016/1‑134 (MQ=255)
                                                                                                     gcACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGAAAGGGCCCCAAGGCAGCTTACACTTTGAACGTGGTGCTTTCAACACGAATGTTTAGCCAAGTTCCATGTAAAGGTTGCCAATGACTGCTTTAATAAAccgt                   >  7:186794/1‑133 (MQ=255)
                                                                                                     gcACGCGCCCTGTGG‑AAGCGGCCTTGTGTCGCTATCGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCtt                   <  4:128480/135‑1 (MQ=255)
                                                                                                     gaaCGCGCCCTGTGC‑AAGCGGCCTTGTGCCGCTAATGGGCCGAATGCCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCtt                   <  7:156660/133‑1 (MQ=255)
                                                                                                      cccggccccTGTGGTACGGGGCGTTGTGGGGCTATCGGCCCGAAAGCCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCtt                   <  8:345640/129‑1 (MQ=255)
                                                                                                               tgtgG‑AAGAGCCCTTGTGTCGCGAAAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGGGCTTTCAACACCAATGTTTAGCCAAGTGCGATGTAAATGGTGCCAATACCAGCGTTAATCAACCGTACGCCGCAtgg        >  6:98772/1‑135 (MQ=255)
                                                                                                               tgtgG‑AACCGGCCTTTTGTCGCGATAGGGCCGCAAGGCCGCTTACACGTTGAACGGGGTGGATTAAACACGAAGGTTTATCCAACTGCCATTTAAAGGTTTCCATTCACATCTTTAATCAACCTAACGCCGCAtg         >  2:181612/1‑135 (MQ=255)
                                                                                                                      tgcggcgTTGTGGCGCTATAGGGCGGCACGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTAt  <  5:349213/135‑1 (MQ=255)
                                                                                                                       gCGGCCTTGTGTCGCTATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTAt  <  7:380878/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                       
CAGTCATGGGGCTTTTTAGTGATGCAGGTGAGCCTGCTTTTTCATGATCGGCATTCGGAAAGTCGGGCGATTGTCGCAGATTTGCGGATTCTCTGCTGCCTGCACGCTCCCTGTGG‑AAGCGGCCTTGTGTCGCGATAGGGCCGCAAGGCCGCTTCCACTTTGACCGTGGTGCTTTCAACACGAATGTTTAGCCAAGTGCGATGTAAAGGTTGCCATTCACTGCTTTAATCAACCTTACGCCGCATGTGAGTAT  >  ACB143/5161192‑5161444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑