Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,300,6550AG38.5% 13.0 / 11.3 30intergenic (‑85/+3488)recC/PP_RS08750RecBCD enzyme subunit RecC/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (13/3);  new base G (10/0);  total (25/5)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAA  >  ACB143/5300523‑5300789
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gCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGATAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGAGGGAGc                                                                                                                                         >  7:71341/1‑135 (MQ=255)
gCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGc                                                                                                                                         >  7:18963/1‑135 (MQ=255)
       aCGATCATGAAGCCCGGGTGTAGAGGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGCGGGCAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGt                                                                                                                                  <  3:226359/134‑1 (MQ=255)
              tGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGGCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCCTGCCCCCTcc                                                                                                                         >  5:326843/1‑136 (MQ=255)
                      gggTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCg                                                                                                                  >  8:252591/1‑135 (MQ=255)
                        gTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑GCCTGGCCCCTGCCGCCCCG‑At                                                                                                               >  3:205248/1‑135 (MQ=255)
                                    ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGCGCGG‑GCGGGGCCGCGCCCGCCCCG‑ATTagcggccggc                                                                                                    >  6:78862/1‑128 (MQ=255)
                                    ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCCCTCCCGCAGCG‑GTTCGCGCAggg                                                                                                     >  1:285860/1‑132 (MQ=255)
                                    ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGGGCCCGCAACCCCAGCG‑ATTAGCGCAGGtg                                                                                                    >  5:287209/1‑133 (MQ=255)
                                         tGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCACCCCCCGCG‑AAAAGCCCAGGTCCA‑Ggtt                                                                                             >  1:348269/1‑135 (MQ=255)
                                           aGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCGTGCCCCCAACCGCAGCG‑CTTAGCGCAAGTCGA‑Ggtttt                                                                                           >  3:332251/1‑135 (MQ=255)
                                              aTCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCGCCCGCCGCG‑GATAGCGCAGGGCGA‑Gtttttttc                                                                                        >  1:317709/1‑133 (MQ=255)
                                                cGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCCG‑GCGTGGCCCCGCCCCCCCCG‑CTTAGCGCACGTCGAGGTTTTTTGccc                                                                                      >  6:2894/1‑136 (MQ=255)
                                                   aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCcac                                                                                    >  2:235464/1‑134 (MQ=255)
                                                   aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGAGGGAGCGG‑GCGTGCCCGCGGCCGCACCG‑ATTAGCGCAGGTGGA‑GTTTTTTTCCcaaa                                                                                   >  5:245579/1‑133 (MQ=255)
                                                        ctctGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCCGGCCCGCACCCGCCGCG‑CTTAGCGCACGGCGT‑GTTTTTTGGCCACAAAGAc                                                                              >  5:72092/1‑135 (MQ=255)
                                                              aGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CGGTGGCCCCGCCCCCCACG‑AATGGCGCAGGGGGG‑GTTTTTTTGCCCCAAAAGCaaaaaac                                                                       >  6:145390/1‑133 (MQ=255)
                                                                   cTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCCG‑CCGTGCCCGCTCCCGCAGCG‑ATTAGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTa                                                                    >  2:304279/1‑134 (MQ=255)
                                                                   cTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCCG‑CCATGCCCGCAACCGCCACG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAACCCAAAACCCCAAc                                                                   >  2:304281/1‑135 (MQ=255)
                                                                   cTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCCGGGGCGG‑CCCGGGCCCCGCCCGCCCCGCATACG‑GCACGTCGACGTTTTTTG‑CCACAAAAACAAAAcaccaaa                                                                   >  6:324636/1‑132 (MQ=255)
                                                                    tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGCCAAAACCCCAAcc                                                                  >  8:279592/1‑135 (MQ=255)
                                                                    tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCCTGCCCGCACCCGCAGCG‑CTTCGCGCAGGTCGA‑GTTTTTTGCCCCCAAAGACAAAACCCCAAcc                                                                  >  8:183580/1‑135 (MQ=255)
                                                                     gtgggCTGAGCGGGCAACGATACCACGCGTACATGAACCGGCCGAATGCCATCAGGCGGCGGGCGCGG‑CGGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCt                                                                 <  2:118739/132‑1 (MQ=37)
                                                                      aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGGGGCCCGGGCCCCCCCCCCCCCCC‑ATAGGCGCCGGGGGG‑GTTTTTTGGCCCAAAAAAAAAAACCCCAAcaac                                                                >  5:26672/1‑133 (MQ=255)
                                                                         ccTGAGAGGGCAACTATATCCCGCGTACCTGAAAGGGCCTAAGCCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGAAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCtgcgt                                                             <  2:275743/134‑1 (MQ=37)
                                                                               agGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGCGCGG‑CCGTGCCCGCTCCCGCCGCG‑CGTCGCGGCGGGGGG‑GGGTTTTTCCCACAAAGACAAAACCCCTACCggggtgcggcg                                                       >  2:342216/1‑132 (MQ=255)
                                                                                                          aCGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCCGGCCCCCGCCCCCCGCG‑CTTCGCGCCGGTGGA‑GTTTTTTTCCCACCAAGACAAAAACCCAAACAGGGGGCGCAGCTAGGGGGTTTTGGAAATGAATCttta                            >  2:230681/1‑133 (MQ=255)
                                                                                                               ccaaTCCCATCTGGCGCCGGGCGCGG‑CGGGCCCCCCCCCCGCCGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATg                       <  7:268551/133‑1 (MQ=17)
                                                                                                                   tGCCATCAGGCGGGGGTAGCGG‑CGGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCt                   <  1:56540/135‑1 (MQ=255)
                                                                                                                                   gAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTTCCCACAAAGACAAAACCCCTACCTGCGGGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGCCCTACTCGCACATGGGGaaa  >  6:89102/1‑136 (MQ=255)
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GCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAA  >  ACB143/5300523‑5300789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑