Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,300,6760AC41.9% 14.1 / 10.1 31intergenic (‑106/+3467)recC/PP_RS08750RecBCD enzyme subunit RecC/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (15/3);  new base C (12/1);  total (27/4)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCG  >  ACB143/5300545‑5300808
                                                                                                                                    |                                                                                                                                      
gggTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCg                                                                                                                                     >  8:252591/1‑135 (MQ=255)
  gTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑GCCTGGCCCCTGCCGCCCCG‑At                                                                                                                                  >  3:205248/1‑135 (MQ=255)
              ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGCGCGG‑GCGGGGCCGCGCCCGCCCCG‑ATTagcggccggc                                                                                                                       >  6:78862/1‑128 (MQ=255)
              ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCCCTCCCGCAGCG‑GTTCGCGCAggg                                                                                                                        >  1:285860/1‑132 (MQ=255)
              ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGGGCCCGCAACCCCAGCG‑ATTAGCGCAGGtg                                                                                                                       >  5:287209/1‑133 (MQ=255)
                   tGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCACCCCCCGCG‑AAAAGCCCAGGTCCA‑Ggtt                                                                                                                >  1:348269/1‑135 (MQ=255)
                     aGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCGTGCCCCCAACCGCAGCG‑CTTAGCGCAAGTCGA‑Ggtttt                                                                                                              >  3:332251/1‑135 (MQ=255)
                        aTCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCGCCCGCCGCG‑GATAGCGCAGGGCGA‑Gtttttttc                                                                                                           >  1:317709/1‑133 (MQ=255)
                          cGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCCG‑GCGTGGCCCCGCCCCCCCCG‑CTTAGCGCACGTCGAGGTTTTTTGccc                                                                                                         >  6:2894/1‑136 (MQ=255)
                             aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCcac                                                                                                       >  2:235464/1‑134 (MQ=255)
                             aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGAGGGAGCGG‑GCGTGCCCGCGGCCGCACCG‑ATTAGCGCAGGTGGA‑GTTTTTTTCCcaaa                                                                                                      >  5:245579/1‑133 (MQ=255)
                                  ctctGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCCGGCCCGCACCCGCCGCG‑CTTAGCGCACGGCGT‑GTTTTTTGGCCACAAAGAc                                                                                                 >  5:72092/1‑135 (MQ=255)
                                        aGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CGGTGGCCCCGCCCCCCACG‑AATGGCGCAGGGGGG‑GTTTTTTTGCCCCAAAAGCaaaaaac                                                                                          >  6:145390/1‑133 (MQ=255)
                                             cTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCCG‑CCGTGCCCGCTCCCGCAGCG‑ATTAGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTa                                                                                       >  2:304279/1‑134 (MQ=255)
                                             cTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCCG‑CCATGCCCGCAACCGCCACG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAACCCAAAACCCCAAc                                                                                      >  2:304281/1‑135 (MQ=255)
                                             cTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCCGGGGCGG‑CCCGGGCCCCGCCCGCCCCGCATACG‑GCACGTCGACGTTTTTTG‑CCACAAAAACAAAAcaccaaa                                                                                      >  6:324636/1‑132 (MQ=255)
                                              tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGCCAAAACCCCAAcc                                                                                     >  8:279592/1‑135 (MQ=255)
                                              tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCCTGCCCGCACCCGCAGCG‑CTTCGCGCAGGTCGA‑GTTTTTTGCCCCCAAAGACAAAACCCCAAcc                                                                                     >  8:183580/1‑135 (MQ=255)
                                               gtgggCTGAGCGGGCAACGATACCACGCGTACATGAACCGGCCGAATGCCATCAGGCGGCGGGCGCGG‑CGGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCt                                                                                    <  2:118739/132‑1 (MQ=37)
                                                aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGGGGCCCGGGCCCCCCCCCCCCCCC‑ATAGGCGCCGGGGGG‑GTTTTTTGGCCCAAAAAAAAAAACCCCAAcaac                                                                                   >  5:26672/1‑133 (MQ=255)
                                                   ccTGAGAGGGCAACTATATCCCGCGTACCTGAAAGGGCCTAAGCCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGAAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCtgcgt                                                                                <  2:275743/134‑1 (MQ=37)
                                                         agGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGCGCGG‑CCGTGCCCGCTCCCGCCGCG‑CGTCGCGGCGGGGGG‑GGGTTTTTCCCACAAAGACAAAACCCCTACCggggtgcggcg                                                                          >  2:342216/1‑132 (MQ=255)
                                                                                    aCGGCCGAATGCCATCAGGCTGCGGGGGCGG‑CCCGGCCCCCGCCCCCCGCG‑CTTCGCGCCGGTGGA‑GTTTTTTTCCCACCAAGACAAAAACCCAAACAGGGGGCGCAGCTAGGGGGTTTTGGAAATGAATCttta                                               >  2:230681/1‑133 (MQ=255)
                                                                                         ccaaTCCCATCTGGCGCCGGGCGCGG‑CGGGCCCCCCCCCCGCCGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATg                                          <  7:268551/133‑1 (MQ=17)
                                                                                             tGCCATCAGGCGGGGGTAGCGG‑CGGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCt                                      <  1:56540/135‑1 (MQ=255)
                                                                                                             gAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTTCCCACAAAGACAAAACCCCTACCTGCGGGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGCCCTACTCGCACATGGGGaaa                     >  6:89102/1‑136 (MQ=255)
                                                                                                                            gCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGTAATGAATCTTGACGATGACCTACTCTCACATGTGGAAACCCCACACTAcc         >  8:145218/1‑134 (MQ=255)
                                                                                                                                 cGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGc   >  6:117282/1‑135 (MQ=255)
                                                                                                                                 cGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGc   >  2:1956/1‑135 (MQ=255)
                                                                                                                                 cGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGc   >  6:350409/1‑135 (MQ=255)
                                                                                                                                 cGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACATACTCTCACATGGGGAAACCCCACACTACCATCgg    >  2:245964/1‑134 (MQ=255)
                                                                                                                                  gaaGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCg  <  4:332251/133‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGG‑CCGTGCCCGCTCCCGCAGCG‑ATTCGCGCAGGTCGA‑GTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCG  >  ACB143/5300545‑5300808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑