Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,383,7590TG43.8% 30.1 / 10.8 32intergenic (+529/‑329)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/16);  new base G (14/0);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 5.09e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTT  >  ACB143/5383629‑5383883
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cGTCGGGCGGATGCGGATTAAATCAGTAGACACGGCAACGCATCTACTACCGACAGCGGTGTACTGGCGGACGGGGGATCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCttttt                                                                                                                            <  4:8059/135‑1 (MQ=255)
     ggcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGGCGGTTTTtttg                                                                                                                       >  4:330827/1‑135 (MQ=255)
                       cAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGGCTG‑GAATTTGGGCGCTgg                                                                                                   >  6:394729/1‑135 (MQ=255)
                             acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTGTTTTTTGGCGG‑GGATTTTGGGGGTGCggggg                                                                                              >  1:79083/1‑133 (MQ=255)
                                          tGTAGTACGGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAt‑tt                                                                               <  5:339122/136‑1 (MQ=255)
                                          tGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAt‑t                                                                                <  8:148938/135‑1 (MQ=255)
                                             agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTaa                                                                             <  2:237946/135‑1 (MQ=255)
                                             agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTaa                                                                             <  3:152983/135‑1 (MQ=255)
                                              gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTG‑GGATTTGGGGGGGGGGGGGGGCAAAATAGGAA‑TTaaa                                                                            >  3:93456/1‑134 (MQ=255)
                                              gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTaa                                                                             <  2:136152/134‑1 (MQ=255)
                                              gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGCCTCCACCAAATCCAAAGAAGAAGACGCCCACGGCCGGCGGTTTTTGTGGCCG‑GGATTAGGTGGCGACGGGTCGCAAAATCGGAT‑TTaa                                                                             >  8:231749/1‑134 (MQ=255)
                                               taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAAcc                                                                           <  1:109834/135‑1 (MQ=255)
                                               taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGGCGAAAAATCGGAT‑TTAAcc                                                                           <  4:69562/135‑1 (MQ=255)
                                                    aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTGGCTGTGGATTTTGGTGGGGCGGGGGGAAAAAAAGCTT‑TTAAcccagt                                                                       >  1:210381/1‑132 (MQ=255)
                                                     cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTTGGGG‑GAATTTTGGCGCGGCGGGTGGCAAAAACGGGT‑TTTACCCCgttgt                                                                    >  5:153632/1‑134 (MQ=255)
                                                     cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTGTTTTTTGGGGG‑GGATTTTTGGGCGGGGGGGGGAAAAATCGGGTGTTTACCCCtta                                                                      >  3:370189/1‑134 (MQ=255)
                                                       gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTG‑GTATTTGGGGGGGGCGGGGGGGAAAATCGGAT‑TTTAACCCgttggt                                                                   >  8:54217/1‑132 (MQ=255)
                                                       gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCtt                                                                   <  1:201968/135‑1 (MQ=255)
                                                         ggAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGCGTG‑GAATTTTTGGGCTGCGGGTGGGAAAATCGGAT‑TTAACCCCGTTGTTGTa                                                                >  6:64756/1‑136 (MQ=255)
                                                          gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTG‑GAATTTGGGGGGGGGGGGGGGGAAAAAAGGAT‑TTAAACCCGTTATTGttg                                                               >  6:266737/1‑134 (MQ=255)
                                                           aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCTG‑GGATTTTGGGGCTGCGGGGGGAAAAATAGGGG‑TTAAACCCCTTGGTGTAgg                                                              >  6:95021/1‑136 (MQ=255)
                                                                   cGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGtt                                                         <  4:157529/133‑1 (MQ=255)
                                                                         ggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCaaat                                                 <  8:1609/135‑2 (MQ=255)
                                                                                          ggCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTGGGGG‑GGATTTTGGGGGGGGGGGGGGCAAAAAAGGAT‑TTTAAACCTTTGTTTTTGGGGGGGTTACAAAGATACTGCCTGCGccggg                                >  4:273238/1‑132 (MQ=255)
                                                                                          ggCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTTGGTG‑GTATTTTGGCGCTGCGGGGCGAAAAATCGGGG‑TTTACCCCGTTGTTGTATGGGGGTTGGCAAAGATTCTGCCGGCGCCGGt                                >  7:79965/1‑135 (MQ=255)
                                                                                                    aaTCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCGG‑GTATTTTGGGGCGGGGGGGGGGAAAAAAGGAT‑TTTAACCCGTTTTTTTTTGGCGTTTGCCAAATATACTGCCTGCGCCGGTTTTGCGtttc                      >  8:207077/1‑133 (MQ=255)
                                                                                                      tacAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                    <  2:187878/133‑1 (MQ=255)
                                                                                                        cAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGGTG‑GGATTTTGGGGGTGGGGGTGGTAAAATAGGAT‑TTTAAACCATTCTTTTTTGGGGGTTTCCAAAGAAAATTCCTGCGCCGTTTTTAAGTTTCGGtg                  >  8:216435/1‑134 (MQ=255)
                                                                                                             aagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt            <  6:371482/136‑1 (MQ=255)
                                                                                                              ataagaCTCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt            <  7:63955/133‑1 (MQ=255)
                                                                                                              agaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGTGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt            <  2:306128/135‑1 (MQ=255)
                                                                                                              agaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt            <  7:232025/135‑1 (MQ=255)
                                                                                                                       ccTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGTGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt  <  6:269406/136‑1 (MQ=255)
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CGTCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTT  >  ACB143/5383629‑5383883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑