Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,768,3280TG39.6% 57.4 / 10.2 53E610A (GAG→GCG) caiTL‑carnitine/gamma‑butyrobetaine antiporter
Reads supporting (aligned to +/- strand):  ref base T (6/26);  new base G (21/0);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.14e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTG  >  ACB143/5768195‑5768461
                                                                                                                                     |                                                                                                                                     
cTGATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctc                                                                                                                                      <  1:115159/135‑1 (MQ=255)
cTGATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctc                                                                                                                                      <  1:121196/135‑1 (MQ=255)
    tCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                 <  5:320857/136‑1 (MQ=255)
     cGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                 <  7:62501/135‑1 (MQ=255)
      gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCgg                                                                                                                                >  4:334636/1‑135 (MQ=255)
      gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCAGCGCGCgg                                                                                                                                >  3:95856/1‑135 (MQ=255)
      gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGAGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCCCgg                                                                                                                                >  4:220377/1‑135 (MQ=255)
      gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACACCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGAGCCTCCCTCTgc                                                                                                                               >  6:306150/1‑136 (MQ=255)
       aGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCgg                                                                                                                                <  1:10140/134‑1 (MQ=255)
                cgcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGGGGCCCGGcccg                                                                                                                       >  7:294182/1‑132 (MQ=255)
                cgcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGccct                                                                                                                       >  8:375332/1‑132 (MQ=255)
                cgcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGcccga                                                                                                                      >  5:182792/1‑132 (MQ=255)
                cgcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCag                                                                                                                       >  7:74141/1‑133 (MQ=255)
                     gATCTGGTCTGGGGCAAAGACCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGTCGCCGCGCGCGCCGCCGCCCTATAAcc                                                                                                                 >  8:85254/1‑135 (MQ=255)
                         tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGTCTCACCCTCGACCGGCGCCTCGCGCGGCGCGGCCATAAAGACggaaa                                                                                                            >  6:71394/1‑132 (MQ=255)
                         tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGGCGCGCGGGGCGAGGCCATATAGCAGTCaa                                                                                                            >  6:167178/1‑136 (MQ=255)
                             ctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGc                                                                                                         <  2:364117/135‑1 (MQ=255)
                              tggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCCCAGGGCGCGGCCATATACACGTCAACGca                                                                                                        >  7:320341/1‑134 (MQ=255)
                              tggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACATCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGGGCGCGCGGCGCGGCCAGATAGCCGGCAggggt                                                                                                        >  8:38634/1‑133 (MQ=255)
                               ggggCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGCCGCCCGCGGCGCCGCCCTATACCCGTCAAAGCtaa                                                                                                      >  6:72943/1‑134 (MQ=255)
                                    aaaGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCTAGAGCCGGCACAGCGCACTTCg                                                                                                 >  6:59706/1‑136 (MQ=255)
                                        cccATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGCTCGCGCGGCGCGGGCCGAAAGCCGGCAACGCTCACTTCGTAg                                                                                              >  5:205496/1‑135 (MQ=255)
                                        cccATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGGCAGATAGCCGGCAAGGCGCCCATCGTAg                                                                                              >  2:7856/1‑135 (MQ=255)
                                        cccATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCCCGCGGCGCGGCCCGCAAGCCGGCAAGGCTCCCTTCGTAg                                                                                              >  8:133727/1‑135 (MQ=255)
                                         ccagccggTCGTAATCCTGACTGCCGTCATGCAGTTAGACCTCGGCGCTTTTGAAGAGCTGGGGCAGTTCAGGCTCACCCTCGACCGGCGGCTCGCGCGGCGCGGCAATATAGCCGTCAAGGCTCACTTCGTCGa                                                                                             <  3:11275/129‑1 (MQ=255)
                                              aGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAAc                                                                                        <  5:279415/135‑1 (MQ=255)
                                              aGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCGATACACGACAAGGCTCCCTTAGTAGACgcaa                                                                                        >  8:76500/1‑132 (MQ=255)
                                               ggTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAAc                                                                                        <  3:131481/134‑1 (MQ=255)
                                               ggTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACg                                                                                       <  7:76500/135‑1 (MQ=255)
                                                 tCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACggg                                                                                     <  2:240228/135‑1 (MQ=255)
                                                   ggaCTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                   <  6:359788/133‑1 (MQ=255)
                                                   gTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                   <  5:92550/135‑1 (MQ=255)
                                                   gTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                   <  6:182695/135‑1 (MQ=255)
                                                    tACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                   <  8:30941/134‑1 (MQ=255)
                                                    tACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                   <  4:21340/134‑1 (MQ=255)
                                                     aCTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGGCGCGCGCGGCGCGGCCCGCTAGCCGGCAACGCTGACATCGGAGGCGAACGGGGTgg                                                                                 >  1:341286/1‑134 (MQ=255)
                                                     aCTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGc                                                                                 <  7:322102/135‑1 (MQ=255)
                                                        ccTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCGATAGCCGTCAAGGCTCACTACGTAGACGAACGGGGTGgccgc                                                                             >  5:158375/1‑134 (MQ=255)
                                                            aCTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCAAGTCGTAGACGAACGGGTTGCCcgctgc                                                                          >  1:344670/1‑133 (MQ=255)
                                                                 cGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCcat                                                                       <  4:119141/133‑1 (MQ=255)
                                                                      tGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCAAATAGCCGTCAAGGCTCACGTCGTAGACGCACGGGTGGCGCTCGTCCAGCATCAccc                                                               >  6:72541/1‑136 (MQ=255)
                                                                         aGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCg                                                              <  2:354739/134‑1 (MQ=255)
                                                                           gTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGCGCGCGCGGCGCGGCCCGAAAGCCGTAAAGGCGCACGGCGGAGAAGAACCGGGTGCGCGCGGCCCTCCTCAccccccc                                                           >  2:239213/1‑131 (MQ=255)
                                                                                     gcgcGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAgg                                                 <  8:191599/135‑1 (MQ=255)
                                                                                       gcgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGtt                                               <  1:239213/135‑1 (MQ=255)
                                                                                                        gcAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCTATATCCGTGAAAGCGCACATAGTTGACGAACCGGGTGGCCTCGTCCATCATAACCCCCCACCCCAACACGTTGCGCGGCTGGCCCAcac                              >  4:286237/1‑133 (MQ=255)
                                                                                                            ttCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGt                          <  7:362492/135‑1 (MQ=255)
                                                                                                            ttCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGt                          <  7:282861/135‑1 (MQ=255)
                                                                                                            ttCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGt                          <  2:229025/135‑1 (MQ=255)
                                                                                                                  cTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCAcc                   <  5:216045/136‑1 (MQ=255)
                                                                                                                         tCGACCGGCGCCGCGCGCGGCGCGGCCCTATAGCCGTCAACGCTCACTTCGTAGACGAACGGGGTGGGCGCGTGCATCATCACCCCCAACCCCCACAGGTTGCGCGCCTGGGCCACCCCGGGTTGCACCACGAAGc            >  6:72900/1‑136 (MQ=255)
                                                                                                                            aCCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  2:341286/133‑1 (MQ=255)
                                                                                                                            aCCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  1:249717/133‑1 (MQ=255)
                                                                                                                            aCCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  3:10291/133‑1 (MQ=255)
                                                                                                                                 cgcCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGt     <  1:48164/135‑1 (MQ=255)
                                                                                                                                   ccTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTg  <  5:218218/136‑1 (MQ=255)
                                                                                                                                   ccTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACGTCGTAGACGACCGGGGTGCCCGCGTCCATCATCACCCGCACGCCCAACACGTTGCGCGCCTGGGCAACGCGGGGGTCCACCTCGAAGCCCTGCGGCt   >  8:223434/1‑135 (MQ=255)
                                                                                                                                    ctcgctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGACCGGGTTGGCCGCTTCCATCATAACCCCCCAGCCCAAAAGGTTGCGCGCCTGGCCAACGCGGGTTTCCCCCTCGAAGCCCTGCGTCt   >  8:287052/1‑134 (MQ=255)
                                                                                                                                    ctcgctcgGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTg  <  4:305595/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CTGATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCATCACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTG  >  ACB143/5768195‑5768461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑