Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,995,7100TG51.7% 13.0 / 27.8 29*210Y (TAA→TAC) ycaC_4putative hydrolase YcaC
Reads supporting (aligned to +/- strand):  ref base T (7/7);  new base G (15/0);  total (22/7)
Fisher's exact test for biased strand distribution p-value = 2.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-03
Rejected as consensus: Frequency below/above cutoff threshold.

TGTTCATCGGCGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGG  >  ACB143/5995586‑5995831
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tgtTCATCGGCGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCCCCGCTTGGCGGTG‑CCCGCGGCGCTTGGGCGTACTtg‑gtgg                                                                                                                 >  7:123582/1‑135 (MQ=255)
tgtTCATCGGCGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCCCCCATTTGCGGTG‑GCCGCGGCGATTGGGGGTACgtg‑tttg                                                                                                                 >  4:106082/1‑133 (MQ=255)
  ttCATCGGCGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCTCCGATTGGCGGTG‑CCCGCGGCGAGTGGGCGTACTTG‑TTGGCg                                                                                                               >  3:39231/1‑135 (MQ=255)
    ctgcGGGGTTCTTTGGGATGAGCTGGCCCCCTCCCAGGGT‑CCACGGGGGCCGGGGCGGTGTATGGGATTGGTGGGCGCCCCGGGGGGGGCCCGGTTGGGGGGGG‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGtt                                                                                                             <  8:322252/132‑1 (MQ=255)
        ggCGTTGGTTGGTTTGAGCTGGCCCGATTCCACTGTGCACCGGCGGAG‑GGGGGGTGTTTTGTTTTGGGGGGC‑GGCCGGGGGGGGGAACGTTTGGGGGGGCCCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAg                                                                                                         <  2:253700/135‑1 (MQ=255)
        ggCGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGGTTGGGCTTGCTTG‑TTGGGGTTGTAg                                                                                                         >  7:59623/1‑135 (MQ=255)
          cGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCGCCGCTTGGCGGTG‑CCCGCGGCGATATGGGGTACTTG‑TTTGGCTTGAAGGc                                                                                                       >  8:312513/1‑135 (MQ=255)
               cTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCCCCGATTGGCGGTG‑CCCGCGGCGATTGGGGTTACTTG‑TTTGCGGTGTAGGCGGTg                                                                                                   >  5:74969/1‑134 (MQ=255)
               cTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGGGGTG‑GCCGGGGGGATTGGGGGTTGTTG‑TTGGGGTTGAAGGggtggt                                                                                                  >  8:183101/1‑135 (MQ=255)
               cTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGCGGTG‑CCCCCGGCGATTGGGGGTACTTG‑TTTGGGGTGAAGGGGTTGt                                                                                                  >  2:416230/1‑135 (MQ=255)
                    gATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCCCCGATTGGCGGTG‑CCCGCGGCGAGTTGGGGTACTTG‑TTGGGGGTGTAGGCGGTGTTGCTgg                                                                                            >  5:117439/1‑136 (MQ=255)
                           ttGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCCCCGCTTGGCCGTG‑CCCGCGGGGCTTTGGGGTTCTTG‑TTTGCGTTGAAAGCGTTGTTGCTGGtcatga                                                                                      >  2:224247/1‑133 (MQ=255)
                                              aaCGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCCATTGGCTGTG‑CCCGCCGGGATTTGGGGTAATTG‑TTGGGGTTGAAGGCGGTGTTGCAGGTAATAAGGGTGCGGTAGTCCcggct                                                                   >  4:183365/1‑133 (MQ=255)
                                                cggcggAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCCATTGGCGGTT‑CCCGCGGCGATTGGGGGTAATTT‑TTGGCGTTGAAGGCGTTGTAGCTGGTGATGAAGTTGCGGTAGTCCCGGAtgt                                                                 >  8:30604/1‑135 (MQ=255)
                                                   cggAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGATTGGGCGTACTTT‑GTGGCGTTGAAGGCGTTGTAGGTGGTCATCAGGTTGGGGTAGTCCGGGAggtggt                                                              >  4:101687/1‑135 (MQ=255)
                                                      aC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGATTGGGCGTAATTG‑TTGGCGTTGAAGGCGTTGTAGGTGGTCATAATGTTGCGGTAGTCCGGGTTGTGGtt                                                             >  4:150039/1‑133 (MQ=255)
                                                                              tgctgcCCTGGGCGGGCCCCGATTGGCGGTG‑CCCGCGGCGCTTGGGGGTTGTTG‑TTGGGGGTGTAGGCGGTGTAGGTGGTGATCAGGGTGGGGTTGTGCGGGATGTGGTTGGGGCCCAGCGCGGCCAGGCCTTCg                                    >  8:211383/1‑135 (MQ=255)
                                                                              tgctgcCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGATTGGGGTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCg                                    >  7:359695/1‑135 (MQ=255)
                                                                                ctgcCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGAt                                  >  8:304070/1‑135 (MQ=255)
                                                                                   cccTGGGCGGGCCCCCATTGGCGGTG‑CCCGCGGCGATTGGGGGTACGTG‑TTTGGGTTGAAGGGGTTGTTGGGGGGCATCAGGGTGGGGTTGTGCGGGATTTGGTTGGGGCACCGCGCGGCCCGGGCGTGGATGtg                               >  4:376124/1‑134 (MQ=255)
                                                                                   cccTGGGCGGGCACCGAGTGGCGGTG‑CCCGCGGCGAGTGGGCGGTCTTG‑TTGGGGGTGGAGGGGGTGTTGCTGGTCATCAGGTTGCGGTTGGGCGGGATGTGGTTGGTGCAAACCGCGGCCCCGCCTTAGAGGta                               >  8:362023/1‑134 (MQ=255)
                                                                                           gggcACCGATTGGCGGTG‑CCCGCGGGGATTGGGGTTTCTTGTTTGGGGTTG‑AGGGGTTTGATCTGGTGATCAGGTTGCGGTAGTGCCGGATGTGGTTGGAGCCCAGCGCGGCCAGGCCGTCGAtttcgtttcg                         >  8:209794/1‑133 (MQ=255)
                                                                                                   ttgggCGGGC‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTcgcg                <  6:370349/131‑1 (MQ=255)
                                                                                                     tGGCGGGC‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCgg               <  7:85704/133‑1 (MQ=255)
                                                                                                         gggg‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGc         <  1:99011/132‑1 (MQ=255)
                                                                                                          gggccccGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGc         <  8:359695/131‑1 (MQ=255)
                                                                                                               ccGCGGCGATGGGGCTTACTTG‑TTGGCGGTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGGGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTcaca    <  8:113991/135‑1 (MQ=255)
                                                                                                                 gcggcgATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAgg  >  7:16992/1‑135 (MQ=255)
                                                                                                                 gcggcgATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAgg  >  4:349862/1‑135 (MQ=255)
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TGTTCATCGGCGTTCCTTGGGATGAGCTTGCCCGCATCCACTGTGCAACGGCGGAC‑GGGCGCTGGATTGGATAGGTGTGCTGCCCTGGGCGGGCACCGATTGGCGGTG‑CCCGCGGCGATTGGGCTTACTTG‑TTGGCGTTGAAGGCGTTGTAGCTGGTCATCAGGTTGCGGTAGTCCGGGATGTGGTTGGAGCACAGCGCGGCCAGGCCTTCGATGTCGTTGCGCCAGTCGCGGTGCAGCTCACAGG  >  ACB143/5995586‑5995831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑