Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 1,166 T→C I389T (ATT→ACT)  dnaA → Chromosomal replication initiator protein DnaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,1660TC98.6% 270.7 / ‑5.0 72I389T (ATT→ACT) dnaAChromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (18/53);  total (19/53)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGATTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCTGT  >  ACB143/1036‑1298
                                                                                                                                  |                                                                                                                                    
gAGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt                                                                                                                                  <  6:232030/135‑1 (MQ=255)
 aGCTCCCGCATGACGCAGCCTTTTTCTTCGCTGAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCAGTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt                                                                                                                                  <  2:406140/134‑1 (MQ=255)
 aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTTGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGa                                                                                                                                <  6:221547/136‑1 (MQ=255)
 aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt                                                                                                                                  <  7:316615/134‑1 (MQ=255)
 aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt                                                                                                                                  <  1:315250/134‑1 (MQ=255)
 aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt                                                                                                                                  <  4:307719/134‑1 (MQ=255)
 aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGGCCAACGTCCGTGAGCTGGAAGGTGCACTGAATCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt                                                                                                                                  <  2:256365/134‑1 (MQ=255)
  gCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGa                                                                                                                                <  2:367443/135‑1 (MQ=255)
    tCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAAt                                                                                                                              >  4:263943/1‑135 (MQ=255)
    tCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATc                                                                                                                             >  6:156866/1‑136 (MQ=255)
      ccGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGc                                                                                                                           <  5:139614/136‑1 (MQ=255)
      ccGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGc                                                                                                                           <  6:345357/136‑1 (MQ=255)
       cGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGc                                                                                                                           <  4:178394/135‑1 (MQ=255)
         cATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTc                                                                                                                         >  6:309029/1‑135 (MQ=255)
                     tttttCATCGCTCAGCGCATCCGGTCCAACGTCCGTTAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTg                                                                                                             <  2:88669/135‑1 (MQ=255)
                      ttttCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTAATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGATTAGTGAATCGCTCAAGGATCTGTTgg                                                                                                            >  1:208042/1‑135 (MQ=255)
                        ttCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGGTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGgcg                                                                                                          <  6:332373/135‑1 (MQ=255)
                                tCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAg                                                                                                   >  1:337256/1‑134 (MQ=255)
                                     gcATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  6:100806/136‑1 (MQ=255)
                                     gcATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  6:96332/136‑1 (MQ=255)
                                      cttCCGGTCCCACGTCCGTGAGCTGGAAGGTGGAATGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  4:301707/133‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  2:337256/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  7:231407/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  7:226383/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  7:206034/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  3:241444/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  2:99842/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  2:285065/135‑1 (MQ=255)
                                      cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  2:358574/135‑1 (MQ=255)
                                        tCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTAACGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTgg                                                                                          <  4:396938/135‑1 (MQ=255)
                                          cGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTc                                                                                        <  6:331354/135‑1 (MQ=255)
                                            gTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAg                                                                                      <  1:266848/135‑1 (MQ=255)
                                            gTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAg                                                                                      <  7:370660/135‑1 (MQ=255)
                                            gTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAg                                                                                      <  1:238182/135‑1 (MQ=255)
                                               cAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGTATCTGTTGGCGCTGCAAGACAAACTGGTCAgtgt                                                                                   <  6:254477/135‑1 (MQ=255)
                                               cAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAgtgt                                                                                   <  2:95067/135‑1 (MQ=255)
                                                aaCGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAgtgt                                                                                   <  3:298228/134‑1 (MQ=255)
                                                aaCGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTGATCGCTCACTCGCACTTCATGGGGCGGGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGGCAgtgt                                                                                   <  2:97533/134‑1 (MQ=255)
                                                 aCGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTgg                                                                                 <  7:201842/135‑1 (MQ=255)
                                                   gTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGAt                                                                               <  8:86399/135‑1 (MQ=255)
                                                    tCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATa                                                                              >  8:330782/1‑135 (MQ=255)
                                                         gAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACAt                                                                          >  1:356719/1‑134 (MQ=255)
                                                            cTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTcct                                                                      <  7:330782/135‑3 (MQ=255)
                                                               gAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCg                                                                    <  6:136128/134‑1 (MQ=255)
                                                                aaGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTa                                                                  <  1:6954/135‑1 (MQ=255)
                                                                aaGGTGCACTGAAGCGGGTTATCGCTCACTCGCACATCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCg                                                                    <  2:320759/133‑1 (MQ=255)
                                                                  ggTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTAcc                                                                >  3:250581/1‑135 (MQ=255)
                                                                  ggTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCg                                                               <  6:245392/136‑1 (MQ=255)
                                                                    tGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTc                                                             <  5:230791/136‑1 (MQ=255)
                                                                    tGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTc                                                             <  5:197196/136‑1 (MQ=255)
                                                                     gaaCTGAAGCTGGTTATCGCTCACTTGCACTTCATGGTGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCTTc                                                             <  8:179032/133‑1 (MQ=255)
                                                                     gCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTc                                                             <  1:218755/135‑1 (MQ=255)
                                                                       aCTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCt                                                          >  6:148191/1‑136 (MQ=255)
                                                                        cTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCt                                                          <  4:250581/135‑1 (MQ=255)
                                                                             gCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGtac                                                    <  5:309029/136‑1 (MQ=255)
                                                                              cGGGTTATCTCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGtact                                                   <  5:303052/136‑1 (MQ=255)
                                                                                   tATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTg                                                                                           <  4:252266/91‑1 (MQ=255)
                                                                                   tATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTg                                                                                           >  3:252266/1‑91 (MQ=255)
                                                                                   tATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGtactac                                                 >  6:170498/1‑133 (MQ=255)
                                                                                     tCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTAcaag                                              <  7:304646/134‑1 (MQ=255)
                                                                                        cTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTAcaagat                                            >  7:161408/1‑133 (MQ=255)
                                                                                              cGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGAtct                                    >  1:83619/1‑135 (MQ=255)
                                                                                                 aCTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCg                                 <  8:284488/135‑1 (MQ=255)
                                                                                                   ttCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGAt                               <  7:202563/135‑1 (MQ=255)
                                                                                                                aCATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            <  1:252116/61‑1 (MQ=255)
                                                                                                                aCATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt                                                                                            >  2:252116/1‑61 (MQ=255)
                                                                                                                aCATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGc                  >  8:223401/1‑135 (MQ=255)
                                                                                                                  aTCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGcgt                <  2:49100/135‑1 (MQ=255)
                                                                                                                       cATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTc           >  2:127307/1‑135 (MQ=255)
                                                                                                                             gCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGAACTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTc     >  8:73752/1‑135 (MQ=255)
                                                                                                                               tGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCt    >  1:308802/1‑134 (MQ=255)
                                                                                                                               tGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCTGt  >  5:263782/1‑136 (MQ=255)
                                                                                                                                  |                                                                                                                                    
GAGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGATTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCTGT  >  ACB143/1036‑1298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: