Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | ACB143 | 1,166 | T→C | I389T (ATT→ACT) | dnaA → | Chromosomal replication initiator protein DnaA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 1,166 | 0 | T | C | 98.6% | 270.7 / ‑5.0 | 72 | I389T (ATT→ACT) | dnaA | Chromosomal replication initiator protein DnaA |
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (18/53); total (19/53) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.64e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGATTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCTGT > ACB143/1036‑1298 | gAGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt < 6:232030/135‑1 (MQ=255) aGCTCCCGCATGACGCAGCCTTTTTCTTCGCTGAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCAGTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt < 2:406140/134‑1 (MQ=255) aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTTGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGa < 6:221547/136‑1 (MQ=255) aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt < 7:316615/134‑1 (MQ=255) aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt < 1:315250/134‑1 (MQ=255) aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt < 4:307719/134‑1 (MQ=255) aGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGGCCAACGTCCGTGAGCTGGAAGGTGCACTGAATCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGt < 2:256365/134‑1 (MQ=255) gCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGa < 2:367443/135‑1 (MQ=255) tCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAAt > 4:263943/1‑135 (MQ=255) tCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATc > 6:156866/1‑136 (MQ=255) ccGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGc < 5:139614/136‑1 (MQ=255) ccGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGc < 6:345357/136‑1 (MQ=255) cGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGc < 4:178394/135‑1 (MQ=255) cATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTc > 6:309029/1‑135 (MQ=255) tttttCATCGCTCAGCGCATCCGGTCCAACGTCCGTTAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTg < 2:88669/135‑1 (MQ=255) ttttCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTAATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGATTAGTGAATCGCTCAAGGATCTGTTgg > 1:208042/1‑135 (MQ=255) ttCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGGTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGgcg < 6:332373/135‑1 (MQ=255) tCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAg > 1:337256/1‑134 (MQ=255) gcATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 6:100806/136‑1 (MQ=255) gcATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 6:96332/136‑1 (MQ=255) cttCCGGTCCCACGTCCGTGAGCTGGAAGGTGGAATGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 4:301707/133‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 2:337256/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 7:231407/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 7:226383/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 7:206034/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 3:241444/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 2:99842/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 2:285065/135‑1 (MQ=255) cATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 2:358574/135‑1 (MQ=255) tCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTAACGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTgg < 4:396938/135‑1 (MQ=255) cGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTc < 6:331354/135‑1 (MQ=255) gTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAg < 1:266848/135‑1 (MQ=255) gTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAg < 7:370660/135‑1 (MQ=255) gTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAg < 1:238182/135‑1 (MQ=255) cAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGTATCTGTTGGCGCTGCAAGACAAACTGGTCAgtgt < 6:254477/135‑1 (MQ=255) cAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAgtgt < 2:95067/135‑1 (MQ=255) aaCGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAgtgt < 3:298228/134‑1 (MQ=255) aaCGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTGATCGCTCACTCGCACTTCATGGGGCGGGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGGCAgtgt < 2:97533/134‑1 (MQ=255) aCGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTgg < 7:201842/135‑1 (MQ=255) gTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGAt < 8:86399/135‑1 (MQ=255) tCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATa > 8:330782/1‑135 (MQ=255) gAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACAt > 1:356719/1‑134 (MQ=255) cTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTcct < 7:330782/135‑3 (MQ=255) gAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCg < 6:136128/134‑1 (MQ=255) aaGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTa < 1:6954/135‑1 (MQ=255) aaGGTGCACTGAAGCGGGTTATCGCTCACTCGCACATCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCg < 2:320759/133‑1 (MQ=255) ggTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTAcc > 3:250581/1‑135 (MQ=255) ggTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCg < 6:245392/136‑1 (MQ=255) tGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTc < 5:230791/136‑1 (MQ=255) tGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTc < 5:197196/136‑1 (MQ=255) gaaCTGAAGCTGGTTATCGCTCACTTGCACTTCATGGTGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCTTc < 8:179032/133‑1 (MQ=255) gCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTc < 1:218755/135‑1 (MQ=255) aCTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCt > 6:148191/1‑136 (MQ=255) cTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCt < 4:250581/135‑1 (MQ=255) gCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGtac < 5:309029/136‑1 (MQ=255) cGGGTTATCTCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGtact < 5:303052/136‑1 (MQ=255) tATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTg < 4:252266/91‑1 (MQ=255) tATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTg > 3:252266/1‑91 (MQ=255) tATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGtactac > 6:170498/1‑133 (MQ=255) tCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTAcaag < 7:304646/134‑1 (MQ=255) cTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTAcaagat > 7:161408/1‑133 (MQ=255) cGCACTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGAtct > 1:83619/1‑135 (MQ=255) aCTTCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCg < 8:284488/135‑1 (MQ=255) ttCATGGGGCGTGACATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGAt < 7:202563/135‑1 (MQ=255) aCATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt < 1:252116/61‑1 (MQ=255) aCATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACt > 2:252116/1‑61 (MQ=255) aCATCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGc > 8:223401/1‑135 (MQ=255) aTCACCATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGcgt < 2:49100/135‑1 (MQ=255) cATCGAGCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTc > 2:127307/1‑135 (MQ=255) gCTGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGAACTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTc > 8:73752/1‑135 (MQ=255) tGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCt > 1:308802/1‑134 (MQ=255) tGACTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCTGt > 5:263782/1‑136 (MQ=255) | GAGCTCCCGCATGACGCAGCCTTTTTCATCGCTCAGCGCATCCGGTCCAACGTCCGTGAGCTGGAAGGTGCACTGAAGCGGGTTATCGCTCACTCGCACTTCATGGGGCGTGACATCACCATCGAGCTGATTCGTGAATCGCTCAAGGATCTGTTGGCGCTGCAAGACAAACTGGTCAGTGTGGATAACATTCAGCGTACCGTCGCTGAGTACTACAAGATCAAGATCTCCGATCTGTTGTCCAAGCGTCGTTCGCGTTCTGT > ACB143/1036‑1298 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |