Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,759,509 G→A intergenic (+124/‑16) fpvA_3 → / → ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,5090GA98.2% 188.4 / NA 54intergenic (+124/‑16)fpvA_3/ligM_KToptimizedFerripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/28);  minor base T (0/1);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCT  >  ACB143/4759378‑4759639
                                                                                                                                   |                                                                                                                                  
gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                <  6:271237/136‑1 (MQ=255)
gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                <  5:188596/136‑1 (MQ=255)
 cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                <  7:371706/135‑1 (MQ=255)
 cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                                <  2:244488/135‑1 (MQ=255)
     tGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                              <  7:180848/133‑1 (MQ=255)
       aTCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTaggag                                                                                                                           <  5:190960/134‑1 (MQ=255)
       aTCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGt                                                                                                                         <  5:378143/136‑1 (MQ=255)
         cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGt                                                                                                                         >  1:17558/1‑134 (MQ=255)
         cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGt                                                                                                                         >  1:346476/1‑134 (MQ=255)
              gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACAt                                                                                                                   >  5:22790/1‑135 (MQ=255)
                 gTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                               <  6:224283/136‑1 (MQ=255)
                  ttGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                               >  8:376581/1‑135 (MQ=255)
                      aCGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGccc                                                                                                           <  7:146170/135‑1 (MQ=255)
                       cGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTTTAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGcccc                                                                                                          <  7:245385/135‑1 (MQ=255)
                         cAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGAc                                                                                                       >  5:345914/1‑136 (MQ=255)
                              aaCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCTGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAAcc                                                                                                  <  5:374454/136‑1 (MQ=255)
                                  gCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTgg                                                                                               <  2:17558/135‑1 (MQ=255)
                                              ggTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTgg                                                                                   <  4:205316/135‑1 (MQ=255)
                                                ttttttGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGTATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGgcc                                                                                 <  7:286381/135‑1 (MQ=255)
                                                          tGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCaa                                                                      <  6:345914/136‑1 (MQ=255)
                                                             tGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCaata                                                                    >  3:96407/1‑133 (MQ=255)
                                                                      atcatcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCACCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACAGTTGAAAt                                                          >  5:178083/1‑136 (MQ=255)
                                                                         atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGt                                                        >  1:188257/1‑135 (MQ=255)
                                                                          tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTg                                                      <  5:233606/136‑1 (MQ=255)
                                                                              cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCg                                                    >  1:331892/1‑134 (MQ=255)
                                                                                cGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTaa                                                 >  1:125578/1‑135 (MQ=255)
                                                                                           tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTg                                      <  6:278568/135‑1 (MQ=255)
                                                                                            ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTg                                      <  2:400065/134‑1 (MQ=255)
                                                                                            ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                    >  6:146255/1‑136 (MQ=255)
                                                                                             taaTTGGGAGCGTATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                    <  1:391526/134‑1 (MQ=255)
                                                                                             gAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGAGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                    <  6:191316/135‑1 (MQ=255)
                                                                                             gAATTGTCAGCGGATAACATTATCACACCAACACATAGATTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                                    <  5:259242/135‑1 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGTCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                  >  6:272585/1‑136 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                  >  5:41230/1‑136 (MQ=255)
                                                                                              aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                                  >  6:336294/1‑136 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                               >  4:195804/1‑51 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                               <  3:195804/51‑1 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGt                            >  1:381629/1‑134 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGt                            >  8:237226/1‑134 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc                           >  5:79690/1‑135 (MQ=255)
                                                                                                      gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTAGACATGAGCGCCCCGACTACCCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGt                            >  4:352998/1‑134 (MQ=255)
                                                                                                        ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTa                         >  2:406026/1‑135 (MQ=255)
                                                                                                        ggATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTAc                        >  6:179588/1‑136 (MQ=255)
                                                                                                             aCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCgg                    >  7:238231/1‑135 (MQ=255)
                                                                                                              cAATTTCACACCAACACATAGTGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                   <  1:381351/135‑1 (MQ=255)
                                                                                                              cAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt                   <  1:372375/135‑1 (MQ=255)
                                                                                                                 tttCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgtcgtc               <  5:359733/136‑1 (MQ=255)
                                                                                                                    cacaCCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGTCGACTACGTCTACCCGGTCGTcgc             >  1:222543/1‑135 (MQ=255)
                                                                                                                     acacCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTcgcg            <  1:365082/135‑1 (MQ=255)
                                                                                                                      cacCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCgcgc           <  1:45185/135‑1 (MQ=255)
                                                                                                                      cacCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCACATTGGCGCCTACGTCTACCCGGTCGTcgcg            <  7:288238/134‑1 (MQ=255)
                                                                                                                             acaTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtc   <  6:25295/136‑1 (MQ=255)
                                                                                                                              caTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGtt    >  7:82394/1‑134 (MQ=255)
                                                                                                                               aTAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAATACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTtct  >  3:330175/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                  
GCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCT  >  ACB143/4759378‑4759639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑