Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 1,310,041 (C)5→6 coding (406/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,310,0361.C96.1% 295.4 / 3.8 77coding (401/1989 nt)aaeB_2p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (36/38);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCG  >  ACB143/1309903‑1310163
                                                                                                                                       |                                                                                                                               
cTGTTTCTGCTTACCTTTGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCAACCATTTGCGCAGCGCATGGGCATATGCCATCGTGATGGCCGGCTACACCGCAGCGATCATCCCCTTTCCGGCCATTGaccacc                                                                                                                               >  4:88857/1‑136 (MQ=255)
cTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGaccacc                                                                                                                               <  3:131679/136‑1 (MQ=255)
     tCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCAcccccc                                                                                                                           <  4:89896/135‑2 (MQ=255)
      cTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCAcccccctg                                                                                                                         >  2:278704/1‑133 (MQ=255)
              cTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCAGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTAt                                                                                                                   >  3:239652/1‑135 (MQ=255)
                 ggCGCTTTGGCTTGAGCTGTGTACCGCATAGTCCACCCAGGTGCGCAGCGCCTGGTCCTATGCCTTCGTGCTGGCCGTCTACACCGCAGCGATCATCGCCTTGCCGTCCATTGACCACCCCCCTGCAAGTATTct                                                                                                                <  6:229153/135‑2 (MQ=255)
                     cTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAg                                                                                                           <  2:118781/136‑1 (MQ=255)
                         ggctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                        <  2:246584/135‑1 (MQ=255)
                         ggctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                        <  6:235823/135‑1 (MQ=255)
                         ggctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGcc                                                                                                        <  1:38945/135‑1 (MQ=255)
                           ctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTg                                                                                                     <  2:111414/136‑1 (MQ=255)
                           ctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTg                                                                                                     >  1:232489/1‑136 (MQ=255)
                              gcgcTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                   <  5:344411/135‑1 (MQ=255)
                              gcgcTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                   <  2:176270/135‑1 (MQ=255)
                              gcgcTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGc                                                                                                   <  1:83742/135‑1 (MQ=255)
                                    tgtACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTg                                                                                             <  1:108562/135‑1 (MQ=255)
                                       aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAcc                                                                                         >  6:192757/1‑136 (MQ=255)
                                       aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAcc                                                                                         >  2:149121/1‑136 (MQ=255)
                                        ccGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAcc                                                                                         <  2:232489/135‑1 (MQ=255)
                                            aGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCgaga                                                                                     >  6:288978/1‑135 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  >  8:146064/1‑136 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  <  4:345341/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  <  1:43824/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  <  7:188923/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  <  7:289234/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  <  8:237174/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt                                                                                  <  7:117967/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCGCGCTGTACCGAGATCt                                                                                  <  3:12194/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGACTCGCTGTACCGAGATCt                                                                                  <  7:57352/136‑1 (MQ=255)
                                              cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTg                                                                                 >  2:68938/1‑136 (MQ=255)
                                                 ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGcc                                                                               >  1:56628/1‑136 (MQ=255)
                                                 ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGcc                                                                               >  7:317730/1‑136 (MQ=255)
                                                 ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGcc                                                                               >  3:189931/1‑136 (MQ=255)
                                                 ccACCCAGTTGCGCAGCGCCTGGGCCAATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACAAGGCCGTGGCTCGCTGTACCgagaacagc                                                                                >  1:125567/1‑130 (MQ=255)
                                                   aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg                                                                             >  5:192835/1‑136 (MQ=255)
                                                   aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg                                                                             >  7:278040/1‑136 (MQ=255)
                                                   aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg                                                                             >  2:302096/1‑136 (MQ=255)
                                                   aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg                                                                             >  1:303488/1‑136 (MQ=255)
                                                        gTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTAt                                                                        <  7:46902/136‑1 (MQ=255)
                                                               aGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCtgt                                                                 >  6:149764/1‑136 (MQ=255)
                                                                 cgcCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCtgtg                                                                >  6:55291/1‑135 (MQ=255)
                                                                    cTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCaa                                                             >  7:96244/1‑135 (MQ=255)
                                                                      gggCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcag                                                          >  2:354436/1‑136 (MQ=255)
                                                                       ggCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagc                                                         <  1:222524/136‑1 (MQ=255)
                                                                         ccTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca                                                       <  7:330457/136‑1 (MQ=255)
                                                                         ccTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca                                                       <  5:288978/136‑1 (MQ=255)
                                                                         ccTATGCCGTCGTGCTGGCCGGCTTCACCGGAGCGATCATCGCCGTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca                                                       <  2:276072/136‑1 (MQ=255)
                                                                             tGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCcagcag                                                   >  5:15907/1‑136 (MQ=255)
                                                                              gCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGc                                                                                >  2:170881/1‑106 (MQ=255)
                                                                              gCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGc                                                                                <  1:170881/106‑1 (MQ=255)
                                                                               cctgattGCTGGCCTGCTACACCTAAGCGATCATCGACTTGCCGGCCATTTACCCCCCCCCTGCAAGTATTCGACCAGGCCTTGGCTCGCTGTACCGAGATCTGACTGGGTATTTTCTGTTCAAAAGCCAGCAGt                                                  <  3:112403/129‑1 (MQ=255)
                                                                               ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                  <  1:231132/135‑1 (MQ=255)
                                                                               ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                  <  2:56628/135‑1 (MQ=255)
                                                                               ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                  <  1:29642/135‑1 (MQ=255)
                                                                               ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                  <  5:218068/135‑1 (MQ=255)
                                                                               ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt                                                  <  5:185780/135‑1 (MQ=255)
                                                                               ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTg                                                 >  3:78799/1‑136 (MQ=255)
                                                                                cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGc                                                >  7:63296/1‑136 (MQ=255)
                                                                                cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGc                                                >  7:148371/1‑136 (MQ=255)
                                                                                cTTCGTGCTGGCCGGCTACAACGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTAATCGACCAGGCCGTGGCTCGCTGGACCGAGATCTGCCTGGGTAATTACTGTGCAACAGCCAGCAGTGc                                                >  4:185893/1‑136 (MQ=255)
                                                                                     tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc                                           >  8:110900/1‑136 (MQ=255)
                                                                                     tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc                                           >  7:281612/1‑136 (MQ=255)
                                                                                      gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTAt                                                                        >  1:252350/1‑106 (MQ=255)
                                                                                      gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTAt                                                                        <  2:252350/106‑1 (MQ=255)
                                                                                      gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGct                                          >  6:84206/1‑136 (MQ=255)
                                                                                      gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGct                                          >  1:82558/1‑136 (MQ=255)
                                                                                      gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGctc                                         >  7:302433/1‑136 (MQ=255)
                                                                                       cTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGctc                                         <  7:156647/136‑1 (MQ=255)
                                                                                            cGGCTACACCGCCGCGATCCTCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTAGTCGACCAGGCCGTGGCTCGCTGTACCGAGAGCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGcc                                    <  8:308853/136‑1 (MQ=255)
                                                                                            cGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGcc                                    <  4:176304/136‑1 (MQ=255)
                                                                                                  cacCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCg                              >  5:134399/1‑136 (MQ=255)
                                                                                                  cacCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCg                              >  8:260298/1‑136 (MQ=255)
                                                                                                      gCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTc                          <  8:112619/136‑1 (MQ=255)
                                                                                                        aGCGAGCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTCTTCGCCCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTc                          <  8:100878/134‑1 (MQ=255)
                                                                                                           gATCATCGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGag                       >  3:48552/1‑134 (MQ=255)
                                                                                                            atcatcGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGCGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGagcag                    <  1:260712/136‑1 (MQ=255)
                                                                                                             tcatcGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCGGGGTATTTTCTGTGCAACAGCCAGCAGAGCCTTGCTCTGGCCCATGCGGGTCGagcag                    >  8:132298/1‑135 (MQ=255)
                                                                                                               atcGCCTTGCCGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGAGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCaa                 >  3:261645/1‑136 (MQ=255)
                                                                                                                         cGGCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTgggcgggc       <  1:274828/136‑1 (MQ=255)
                                                                                                                          ggCCATTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACGgggcggg        >  1:128998/1‑134 (MQ=255)
                                                                                                                              aTTGACCACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGgcg  >  7:30353/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                               
CTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACCACCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGCG  >  ACB143/1309903‑1310163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: