Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143675,1240TG46.7% 14.2 / 12.0 30Q400P (CAG→CCG) mcpH_2Methyl‑accepting chemotaxis protein McpH
Reads supporting (aligned to +/- strand):  ref base T (12/4);  new base G (0/14);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 2.21e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTCGCGGGCCTGCTGCTGGCCGCTATCGGCCGACTGCGCCGCCTGGCTGCAGGACCGTGCCACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTT  >  ACB143/674999‑675257
                                                                                                                             |                                                                                                                                     
cTTCGCGGGCCTGCTGCTGGCCGCTATCGGCCGACTGCGCCGCCTGGCTGCAGGACCGTGCCACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGcggccggc                                                                                                                             >  7:78878/1‑136 (MQ=255)
             ctgctgGCCGATATCGGCCGACTGCGCCGCCTGGCTGCAGGACCGTGCCACTTCATTGGCGGTGGCGACCATTTCGTGTAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATg                                                                                                                <  2:229604/136‑1 (MQ=255)
                             gCCGACTGCGCCGCCTGGCTGCAGGACCGGGCCACTTCATTGGGGGGGGCGACCATTTCGTGGACTGCGGTGGAAACCATGTCCCCGGCGTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCggg                                                                                                <  2:275589/136‑1 (MQ=255)
                              ccGACGGCGCCGCCGGGGTGCAGTACCGGGCCAATTCATTGGGGGGGGCGACAATTTCGGGTAAGGCGGTGTACACCATCTCCCCGGCTCCGCGCTGGCGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGt                                                                                               <  2:253480/136‑1 (MQ=255)
                                     cgccgcCTGGCTGCAGGACCGTGCCACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGCgctg                                                                                        >  6:309462/1‑136 (MQ=255)
                                        cgcggggCTGCTGCACGGGGCCACTTATTTGGGGGGGGCGACATTTTCGTGCATGGGGGTGCAAACCAGGGCCCGGGGTGCGCGCGGGGGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGctgctg                                                                                     <  8:178548/131‑1 (MQ=255)
                                               cggCAGGACCGTGCCACTTATTTGGCGGGGGCGACCATTTCGTGGAAGGCGGTGTAAACCAGGTCCAGGGCTGCGCGCGGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGc                                                                              <  2:118456/134‑1 (MQ=255)
                                                    ggcccGTCCCACTTCATGGGCGGTGGCGACCATTTCGTGTACTGCGGTGCAAACCCTGTCCCGGGCTGCGCGCGGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAgg                                                                         <  4:77989/133‑1 (MQ=255)
                                                     tacggggCCACTTCTTTGGGGGGGGCTACCATTTGGTGGATTGCGGTGCAAACCATGACCAGGGCTGCGGGCGGGCGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAgg                                                                         <  1:16020/129‑1 (MQ=255)
                                                     gACGGTGCCACTTCATTGGGGGTGGCTACATTTTCGTGTATTGCGGTGGAAACCATGTCCAGGGGTTCGGGCGGGGGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAg                                                                          <  5:157659/134‑1 (MQ=255)
                                                         gTGCCACTTCATTGGCGGGGGCTACCATTTGGTGGAAGGCGGGGCAAACCATGCCCCCGGCTTCGCGCGGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCtt                                                                    <  4:295038/136‑1 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTg                                                                >  8:8417/1‑136 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTg                                                                >  7:191413/1‑136 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTg                                                                >  4:251963/1‑136 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTg                                                                >  4:135775/1‑136 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTg                                                                >  2:295351/1‑136 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTg                                                                >  2:260037/1‑136 (MQ=255)
                                                             cACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCGCCGCCAGGGCACCGGAGCCGCGGGTCGCGCTGCGGGACGTGCGGAGGCTCGTGCTg                                                                >  3:185537/1‑136 (MQ=255)
                                                                  cATTGGCGGTGGGGAACATTTCGTGTATTGCGGTGGAAACATTGCCCCCGGCTTCGCTCGGGCGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGgcag                                                            <  8:261795/135‑1 (MQ=255)
                                                                      ggggggggCGACCTTTTCGTGTACTCGGGGGTAAACCTGGCCCCGGGCTGCGGGCGGGCGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTg                                                       <  2:340174/130‑1 (MQ=255)
                                                                      ggCGGGGGCGACCATTTCGTGCAATGCGGGGCAAACCTGGCCCCGGGCTTCGCGCGGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTg                                                       <  1:78848/136‑1 (MQ=255)
                                                                           tGGCGACCATTCGGTTGATGGCGGTGTAAACCATCTCCACGGCTGCGCGCGGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGAt                                                  <  7:41814/136‑1 (MQ=255)
                                                                           gggCTACCATTTCGTGTATTGCGGGGGAACCTTTGGCCAGGGTTGGGGGGGGGGGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGAt                                                  <  1:13922/135‑1 (MQ=255)
                                                                             gCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGAtgt                                                >  8:156048/1‑136 (MQ=255)
                                                                                       cggggAAGGGGGTGGACACTTTGTCCACGGGTGCGCGGGGGGGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAg                                      <  2:348930/133‑1 (MQ=255)
                                                                                                ggttgtaaaCCTTGCCCCGGGCTTGGGGCGGGGGGCCGGCGGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGaa                              <  2:27157/129‑1 (MQ=255)
                                                                                                         ccttGTCCAGGGGTTGGCGCTGGGGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAg                    <  3:117139/133‑1 (MQ=255)
                                                                                                                 ccGGCTGGGCGCGGGCGGCGGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGt            <  8:213235/135‑1 (MQ=255)
                                                                                                                           gcTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGtt  >  8:133472/1‑136 (MQ=255)
                                                                                                                           gcTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGtt  >  1:318376/1‑136 (MQ=255)
                                                                                                                             |                                                                                                                                     
CTTCGCGGGCCTGCTGCTGGCCGCTATCGGCCGACTGCGCCGCCTGGCTGCAGGACCGTGCCACTTCATTGGCGGTGGCGACCATTTCGTGGAATGCGGTGGAAACCATGTCCACGGCTTCGCGCTGGCGGCCGGCCGCCTCGGCCATGTCACTGGAGACGCGGGTCGAGCTGCTGGACGTGCTGAGGATCTTGCTGGCAGCAGTGCCGATGTGCTGGATCAGGCTGCGAATGGCACCGAGGAACTGGTTGAACCAGTT  >  ACB143/674999‑675257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑