Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,984,5440GC40.0% 30.3 / 11.0 30G237G (GGC→GGG) CJ019_03762hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/7);  new base C (0/12);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 6.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGCCCAAC  >  ACB143/3984415‑3984679
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tttCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGAGACCAT‑GGCGAAGCCACgg                                                                                                                                   >  7:336386/1‑136 (MQ=255)
   cATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGACGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCt                                                                                                                                 >  5:298875/1‑135 (MQ=255)
            cGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTg                                                                                                                       >  5:136460/1‑136 (MQ=255)
             gCCAGCCTGTCGCCATGCGGCCCGTGGTGTTTCGCCAACCCTGGCCGGGCAGGGGTGATCATGACGCGCAACCCCCGGGTGGCGTCGTACTGGGCCGGCCCGGCGACCAG‑GGCGACCCCACGGCTCATCTGGTTGc                                                                                                                      <  2:90824/136‑1 (MQ=255)
              ccAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGcc                                                                                                                     >  8:39698/1‑136 (MQ=255)
                  cgtGTCGCCCTGCGGCCCGTGGTCTTTCTCCAACACTTGGCCGTCATGGCTTATCATCACGCTCATCCCCCGGGGGGCGGCGTACTGGCCCGGCCCGGCGACCAG‑GGCGAACCCACGGCTCATCTGGTTGCCGATc                                                                                                                 <  8:23965/134‑1 (MQ=255)
                     gTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAgg                                                                                                              >  8:209664/1‑136 (MQ=255)
                                              tcGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCg                                                                                      >  7:178642/1‑135 (MQ=255)
                                                 aaCCCTTGCCCGTCATGGCTGATCATCAACCCCAGCACCCCGGGGGCGGCGTACTGGGCCGCCCCGGCGACCAT‑GGCCACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATg                                                                                  <  5:305316/136‑1 (MQ=255)
                                                 aaCACTGGGCCGACCGGGCTGAACATCACCCACACCCCCCCGGGGCGGCGGTACTTGCCCGGCCCGGCG‑CCATGGGCCACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATg                                                                                  <  8:19381/136‑1 (MQ=255)
                                                   cacTTGGCCGTCATGGCTTATCATGACGCTCATCACCCCGGTGGCGTCGACCTGGGCCGGCCCGGCCACATG‑GGCGACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATggg                                                                                <  7:182915/136‑1 (MQ=255)
                                                                 ggatGATCTTCATGCTCATCACCCGGGTGGGGTGGTAGTTGGCCGCCACGGCTACCTT‑GGGCATGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCa                                                                   <  2:273093/132‑1 (MQ=255)
                                                                 ggCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAg                                                                  >  8:59486/1‑136 (MQ=255)
                                                                      aTCATTACGCTCATCACCCGGGTGTCGTGTTAGTTGGCCGGCCTGGCGACCTT‑GGCTACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATg                                                              <  7:39698/135‑1 (MQ=255)
                                                                        aatcaaGCTCATCCCCGCGTTGTGGTCGTCGTGGGCCGGCCAGGCTCCCTT‑GGCTAACCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCgg                                                           <  2:94066/132‑1 (MQ=255)
                                                                         atgacccTCATCACCGCGGGGTCGTGGTACTTGGCGGCCCTGGCGACCAT‑GGCGAACCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGa                                                          <  4:220540/130‑1 (MQ=255)
                                                                         aTGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCgg                                                           >  3:32110/1‑135 (MQ=255)
                                                                               cTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGt                                                    >  1:330902/1‑136 (MQ=255)
                                                                                     aCCCCGGTGGCGTCCAACTGGCCCGCCCGCCCCACCTC‑GCCGAACCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCAt                                              <  2:146802/136‑1 (MQ=255)
                                                                                          gttgtCGGCGACCTGGGCCGGCCTGCCGACCCG‑GGCCACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAg                                         <  2:97760/134‑1 (MQ=255)
                                                                                            tggcggcgtACTTGGCCGCCCCGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGc                                       <  7:49318/130‑1 (MQ=255)
                                                                                             ttcgtctTAGTGGCCCGCCCCGGCCACCAG‑GGCGACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCg                                      <  5:144264/135‑1 (MQ=255)
                                                                                              tcgtcgTCCTGGGCGGGCCCGCCGACATG‑GGCGAACCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                     <  7:185998/136‑1 (MQ=255)
                                                                                              tcggcgTCCTTGGCCGGCCCGGCGACCTT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                     <  5:336485/136‑1 (MQ=255)
                                                                                              ggggcgtAGTGGGCCGCCCCCGCTACCAG‑GCCCACGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                     <  3:225394/132‑1 (MQ=255)
                                                                                               cgtcgtACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGt                                     >  6:182318/1‑135 (MQ=255)
                                                                                                    tACTGGGCCGGCCCGGCGACCTG‑GCCCACCCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCg                                <  4:159059/135‑1 (MQ=255)
                                                                                                             ggccAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCt                      >  3:159059/1‑136 (MQ=255)
                                                                                                                     gACCTT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAAc              <  3:88154/136‑1 (MQ=255)
                                                                                                                       cctt‑GGCCAACCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAg            <  2:281971/133‑1 (MQ=255)
                                                                                                                                  gCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGCCCAAc  <  4:50984/136‑1 (MQ=255)
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TTTCATCTCCTTCGCCAGCCTGTCGCCATGCGGCCCGAGGTCTTTCTCGAACACTTGGCCGTCATGGCTGATCATGAAGCTCATCACCCCGGTGTCGTCGTACTTGGCCGGCCAGGCGACCAT‑GGCGAAGCCACGGCTCATCTGGTTGCCGATCAGGTAGCTGTAGGCACCGCCCGGGGCGGATGGGCCCTGTGCATCCAGGATGCGGAAGTGGTAGCCATACCAGGCGTCACCGACCACATCCTGGCCGAACAGCGGGCCCAAC  >  ACB143/3984415‑3984679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑