Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,028,4510TA36.1% 41.6 / 13.4 36intergenic (‑120/‑127)CJ019_03802/mcpU_3hypothetical protein/Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base T (16/7);  new base A (0/13);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 4.95e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCAT  >  ACB143/4028317‑4028579
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gATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAAt                                                                                                                                  >  2:236145/1‑135 (MQ=255)
         aTTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGaaaaaa                                                                                                                        >  5:91861/1‑136 (MQ=255)
                gTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAgg                                                                                                                 >  3:183030/1‑136 (MQ=255)
                   ggCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGc                                                                                                              >  5:275205/1‑136 (MQ=255)
                        aCCTCTTTACTACAGAAAAATGCGCTAACTGTCATCCAGTGGGGGGCAGATGAAAAACGCGCGTTGTTGCGGGGGTTTAAGGGCGAAAAAATTTAAATTTAATTTTTCATTAAATAAAAAACGGCAGGGGCCg                                                                                                            <  6:253464/133‑1 (MQ=255)
                          cTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGatatc                                                                                                       >  7:302135/1‑135 (MQ=255)
                          cTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGatatc                                                                                                       >  5:180178/1‑135 (MQ=255)
                                  gACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAgg                                                                                               >  3:114733/1‑136 (MQ=255)
                                   aCAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAgg                                                                                               >  6:277716/1‑135 (MQ=255)
                                       aaaaaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCg                                                                                          >  1:157654/1‑136 (MQ=255)
                                          aaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAgg                                                                                       >  5:72663/1‑136 (MQ=255)
                                                             agggggCGGCAGATGAAAAACGCGCCTTGATGCGGCGCTTAAACGGCGCACAATTTAAAATTTAATGTTTCAAAAAAAAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCttt                                                                    <  8:155956/133‑1 (MQ=255)
                                                              ttGGGGGGCAGATGACAAACGCGCCTTGTTGCGGCGCTTTAACGGCGTAAAATTTTAAATTGAATGTTTCAATAAATAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTg                                                                   <  8:24558/135‑1 (MQ=255)
                                                              gggggCGGCATATGAAAAACGCGCCTTGTGCCGGCGCTTTAACGCCTCAAAAATTAAAATTTAATTTTACAAAAAAAAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTg                                                                   <  4:136129/134‑1 (MQ=255)
                                                               ggggCGGCTCTTGACAAACGCGCCTTGAGGCGGCGCTTAAACGGGCCAAAATTTTAAATTTATTTTTTAAAAAAAAAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTgg                                                                  <  6:78544/135‑1 (MQ=255)
                                                                  gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAc                                                               >  1:262162/1‑136 (MQ=255)
                                                                  gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAc                                                               >  1:336615/1‑136 (MQ=255)
                                                                  gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAc                                                               >  4:145555/1‑136 (MQ=255)
                                                                          tGTCAAATGCGCCTGCCGGCGGCGCTTTACCGGCGGACAAATTTAAATTGAATTTTTTAAAAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCgg                                                        <  6:96687/135‑1 (MQ=255)
                                                                          tGTCAAAAGCGCCTTGATGCGGCGCTTTAACGGCGGACAAATTTAAATTTAATGTTTGAAAAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCgg                                                        <  5:268076/135‑1 (MQ=255)
                                                                              aaacccgcCTTGAGGCGGCGCTTAACCGGCGCAAAAATTAAAATTGATTTTTTAAAAAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCa                                                   <  3:330414/131‑1 (MQ=255)
                                                                              aaacGCGCCGTGAGGGGGCGCGTAACCGGCGCACAATTTAAATTTTAATTTTTCAAAAAACAAAACCCGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCa                                                   <  6:193168/136‑1 (MQ=255)
                                                                              aaaaGCGCGTTGATGCGGCGCTTTAACGGCGTAAAATTTAAAATTCATTTTTTCAAAAAACAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCa                                                   <  3:317465/136‑1 (MQ=255)
                                                                                   cgcCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCg                                                >  7:243652/1‑134 (MQ=255)
                                                                                    gcCTTTATGCGGCGGTTTAACGGCGCAAAATTTTAAATTGAATGTTTCAATAAATAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAg                                             <  8:187551/136‑1 (MQ=255)
                                                                                    gcCTTGCTGCGGCGGTTAAAGGGCGCAAAAATTAAATTTTATTGTTTAAAAACAAAAAACCCGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAg                                             <  5:127118/136‑1 (MQ=255)
                                                                                    gcCTTGATGCGGCGGTTTAACGGCGTAAAAATTTAAATTCAATGTTTTAAAAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAg                                             <  2:4485/136‑1 (MQ=255)
                                                                                     ccTTGAGGCGGCGGTTAAAGGGCGTAAAAATTTAATTTTAATGTTTCAATAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                            <  6:229400/136‑1 (MQ=255)
                                                                                        tGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCCCCGCAGAtcc                                         >  2:325035/1‑134 (MQ=255)
                                                                                            gcggcgCGTTAACGCCGCAAAAATTAAAATTTATTGTTTCAAAAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGc                                      <  6:80085/135‑1 (MQ=255)
                                                                                                   gtaaaCGGCGTAAAAATTTAAATTCAATTTTTTAAAAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAAccc                              <  4:321807/133‑1 (MQ=255)
                                                                                                              aCAATTTAAAATTTATTTTTTTAAAAAAAAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCaa                    <  3:81079/135‑1 (MQ=255)
                                                                                                                aaaTTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCaaaag                 >  6:196925/1‑135 (MQ=255)
                                                                                                                     tAAATTTATTTTTTTAATAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGa             <  7:315792/135‑1 (MQ=255)
                                                                                                                            aaTGTTTCAATAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGcccc     <  6:309119/136‑1 (MQ=255)
                                                                                                                               gTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCAt  >  7:168283/1‑136 (MQ=255)
                                                                                                                                tataaaTAAAAAAACCACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCAt  <  8:71404/131‑1 (MQ=255)
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GATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTGAATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCCCCAT  >  ACB143/4028317‑4028579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑