Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,067,3540AC46.0% 36.0 / 11.1 37V162G (GTG→GGG) cheB_5Protein‑glutamatemethylesterase/protein‑glutamin e glutaminase
Reads supporting (aligned to +/- strand):  ref base A (1/19);  new base C (17/0);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 1.13e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTA  >  ACB143/6067222‑6067483
                                                                                                                                    |                                                                                                                                 
aaCGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCt                                                                                                                                <  2:79479/136‑1 (MQ=255)
 aCGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCTCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGGCGGCGCCGCCACCtt                                                                                                                               <  8:264307/136‑1 (MQ=255)
   gCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCTGCGccccccccaagg                                                                                                                             >  8:292872/1‑132 (MQ=255)
     cTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCCCCGCCCCCTTGAcc                                                                                                                           >  8:231742/1‑136 (MQ=255)
       tGATCACACAGGGGTACCTGCCACGGGCCAGGAGAAGCTGGCCATGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCg                                                                                                                          <  6:272211/135‑1 (MQ=255)
        gATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCg                                                                                                                          <  1:25304/134‑1 (MQ=255)
           cacacaTGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATGAccccggag                                                                                                                     >  3:246081/1‑130 (MQ=255)
                      gCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCTAGACCGCATCGGGCTTGacccc                                                                                                          >  3:53460/1‑134 (MQ=255)
                      gCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGACGGTGCCGGTGTCGGCGCCGCCACATTGACCGCTTCGGGCTTGACCg                                                                                                           >  6:242817/1‑135 (MQ=255)
                          gCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCTTGACCGCGGAGGGCGTGCCCGCAggc                                                                                                      >  8:14613/1‑134 (MQ=255)
                          gCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCATGACCCCGTGGGGCGTGCCCGCAggc                                                                                                      >  4:317380/1‑134 (MQ=255)
                           ccACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGTTGTGGGCGCCGCCCCCATACCCTCATCGCGCTTGCGCGCacta                                                                                                      >  1:3614/1‑132 (MQ=255)
                           ccACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCCCCCCCATGGCCGCTGCGGGCTGTCCCGCCGtgc                                                                                                     >  6:1855/1‑134 (MQ=255)
                               tGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATTCCCCCGTCGGGCGTGCCCGCAGTgcgcgc                                                                                                 >  8:244254/1‑136 (MQ=255)
                               tGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGGGTCGGCGCCGCCCCCTTGCCCGCGTCGGGCGTGCCCGCCGGCAgggc                                                                                                 >  2:223053/1‑136 (MQ=255)
                                              ggccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGGCGCCCCCTTGACCGCGTCGGGCGTGCCCGCCGTCCGCGCGTTCAACGAGGCGtt                                                                                  >  6:274198/1‑136 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                                 <  1:103299/136‑1 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                                 <  2:178753/136‑1 (MQ=255)
                                               gccctggccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCAGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTg                                                                                 <  3:123432/136‑1 (MQ=255)
                                                     gccACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGTGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAg                                                                           <  7:261181/136‑1 (MQ=255)
                                                       cACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGACCGCGTCGGGGGTGCCCGGCGGCCGCGCGGTGAACGCGGCGTTGGGCAAGGc                                                                         >  8:307993/1‑136 (MQ=255)
                                                       cACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCATGGCCGCGTCGGGGTTGCCCGCAGTGAGCGGGGTGAACGAGTCGGTGGGAACGGc                                                                         >  1:282719/1‑136 (MQ=255)
                                                             cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTGGGCGCCGCCCCCATGACCGCGTGGGGCGTGCCCGCCCTCAGCGCGGTGAACGCGTGGGTTGGAAAGGCCggggt                                                                   >  2:103299/1‑134 (MQ=255)
                                                             cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTAGGCGCCGCCCACGTGACCGCGTCGGGCGTGGCCGCCGTGCGCGGGGTCACAGCGGGGTTGGGAAAGGGCGGGtg                                                                   >  6:21211/1‑135 (MQ=255)
                                                             cGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGGGGGCGCCGCCCCCGTGCCCGCGGCGGGCGGGGCCGCCGGCAGCGCGGGGGACGCGTCGTTGGGGAAGGGCggggt                                                                   >  5:116481/1‑134 (MQ=255)
                                                                  ggCGCGGGGGCGGCAGGTGCGGGCACCGCGACCAAAGGCTTGGCCGGTGCCGGTGTAGGCGCCGCCCCCTTGACCCCGTCGGGCATGCCCGCACTCAGCCCGTGCAACCCCTCGTTTGCAAAGGCCCGGGTGGcgg                                                              >  2:37111/1‑134 (MQ=255)
                                                                                 ggTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATgg                                               <  2:233183/136‑1 (MQ=255)
                                                                                     cGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCa                                            <  5:227744/135‑1 (MQ=255)
                                                                                                  cAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGc                              <  6:112596/136‑1 (MQ=255)
                                                                                                   aaaGGCTTGGCCGGTGCCTGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCt                             <  2:215764/136‑1 (MQ=255)
                                                                                                        cTTGGCCGGTGCCGGTGTCGGCGCCGCCCCCGTGGCCGCGTCGGGCGTGCCCGCCGTCAGCGCGGTGAACCCGTGGTTGGGCAAGGCCGGGGTGGGTCGCGCCCGGCCGCTGGCCCTCAGGGGGTCGGGCGTTCCg                        >  8:279354/1‑136 (MQ=255)
                                                                                                             ccggtgccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCtt                   <  7:249330/136‑1 (MQ=255)
                                                                                                                   ccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCa              <  8:55310/135‑1 (MQ=255)
                                                                                                                   ccggtgTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAg             <  7:170978/136‑1 (MQ=255)
                                                                                                                         tCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctt        <  8:299223/135‑1 (MQ=255)
                                                                                                                           ggCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctttct     <  1:159255/136‑1 (MQ=255)
                                                                                                                             cgccgcCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGctttcttt   <  1:223053/136‑1 (MQ=255)
                                                                                                                              gccgccACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTa  <  1:37616/136‑1 (MQ=255)
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AACGCCTTGATCACACATGGCTGCATGCCACTGGCCAGGCGCAGCTGGCCCTGGCCACGGCCGCTCGGCGCGGGGGCGGCAGGTGCGGGCGCCGCGACCAAAGGCTTGGCCGGTGCCGGTGTCGGCGCCGCCACCTTGACCGCTTCGGGCTTGCCCGCAGTCAGCGCGTTCAACGAGTCGTTGGCAAAGGCCGGGTTGGCTCGCGCCGGGCCGATGGCCATCAGGCTGTCGAGCTTACCGATCTTGGTCAGGGCTTTCTTTA  >  ACB143/6067222‑6067483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑