Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	179,484	T→C	C316R (TGC→CGC)	CJ019_00182 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	179,484	0	T	C	100.0%	269.4 / NA	72	C316R (TGC→CGC)	CJ019_00182	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/58);  total (14/58)

TTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB143/179359‑179614                                                                                                                               |                                                                                                                                   ttCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  3:25136/136‑1 (MQ=255) ttCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  7:132109/136‑1 (MQ=255) ttCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  6:42438/136‑1 (MQ=255)    aGGGCCGGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcgg                                                                                                                       <  2:287617/136‑1 (MQ=255)             ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGCCCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTg                                                                                                               >  3:240595/1‑135 (MQ=255)             ctggcCTAAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTg                                                                                                               >  2:342411/1‑135 (MQ=255)              tggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCGCGCAGCCGCGGCGGCGAGTTTg                                                                                                               >  8:171209/1‑134 (MQ=255)                gcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCa                                                                                                             <  2:54864/134‑1 (MQ=255)                     aaCGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCACCGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  2:63386/136‑1 (MQ=255)                           cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:145488/136‑1 (MQ=255)                           cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:194068/136‑1 (MQ=255)                           cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:242112/136‑1 (MQ=255)                           cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:5399/136‑1 (MQ=255)                           cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:304869/136‑1 (MQ=255)                           cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  8:293418/136‑1 (MQ=255)                           cgccGTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:251380/136‑1 (MQ=255)                            gccTTGGCGTCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGGGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  8:79664/136‑1 (MQ=255)                            gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  2:28871/136‑1 (MQ=255)                              cTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGc                                                                                              >  1:94165/1‑135 (MQ=255)                              cTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGc                                                                                              <  2:94165/135‑1 (MQ=255)                                 ggcggcCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATg                                                                                           <  1:297006/135‑1 (MQ=255)                                     gcCAGCGTACCGACCAGTTGTTGCAAGCTGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCa                                                                                      <  5:156547/136‑1 (MQ=255)                                     gcCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCa                                                                                      <  3:149298/136‑1 (MQ=255)                                       cAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  2:117527/136‑1 (MQ=255)                                       cAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  4:133202/136‑1 (MQ=255)                                       cAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                    <  8:226242/136‑1 (MQ=255)                                        aGCGTACCGACCAGTTGTTGCAATCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGTCTGCGAGTTTGCAGTGCTGGCACCGTGCATGGTGCATga                                                                                   <  6:249581/136‑1 (MQ=255)                                        aGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   <  1:58033/136‑1 (MQ=255)                                              ccGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGc                                                                             <  5:138487/136‑1 (MQ=255)                                                 cccAGTTGTTGC‑ATCGGTACGTGTACACGCTGCGTCGTACTTGTGCCTGCTACCCGCAAACCAACGACATCA‑TTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTa                                                                         <  3:11475/135‑1 (MQ=255)                                                       tgttgCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCt                                                                    >  5:172271/1‑136 (MQ=255)                                                         ttgCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCCACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGGATGAGGAGGCAGTACACCTTg                                                                  <  6:210869/136‑1 (MQ=255)                                                         ttgCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  3:76361/136‑1 (MQ=255)                                                          tgCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  1:215892/135‑1 (MQ=255)                                                               gCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGc                                                                                                     <  6:252146/95‑1 (MQ=255)                                                               gCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGc                                                                                                     >  5:252146/1‑95 (MQ=255)                                                                   tAGGTGAACA‑TCTGCGTCGTAATTCTGCAACCTACCCGGAAACAAACGAACTCATTACACGCAGACGCGGCGCCGAGTTAGCAGTGCTGCCACCGCGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGctct                                                        >  6:92348/1‑136 (MQ=255)                                                                     ggTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTgg                                                      <  5:325855/136‑1 (MQ=255)                                                                     ggTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTgg                                                      <  6:139399/136‑1 (MQ=255)                                                                     ggTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTgg                                                      <  4:98603/136‑1 (MQ=255)                                                                     ggTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTgg                                                      <  3:349790/136‑1 (MQ=255)                                                                         aaCA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                   <  2:101854/135‑1 (MQ=255)                                                                               cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGt                                             <  2:127757/136‑1 (MQ=255)                                                                               cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGt                                             >  1:256727/1‑136 (MQ=255)                                                                                 gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCCGGCTCTGGAGGCAACGTTg                                           >  7:82746/1‑136 (MQ=255)                                                                                 gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTg                                           >  5:56536/1‑136 (MQ=255)                                                                                 gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTg                                           >  6:186135/1‑136 (MQ=255)                                                                                 gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTg                                           >  1:162716/1‑136 (MQ=255)                                                                                     cgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCCTTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACCCCTTGCCCAGGCTCTGGAGGCAACGGTGcc                                         >  8:25853/1‑133 (MQ=255)                                                                                     cgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCCGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCagc                                       >  4:32521/1‑135 (MQ=255)                                                                                            gtgCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGc                                 <  2:256727/135‑1 (MQ=255)                                                                                                cAGCTACCCGTAAACCAACGACCTCATTTCCCGCTGCGGCGGCGGCGTGTTTTGAGGGCTGGGACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCGCAGGCTCTGTAGCCAACGTTGCAGAGCCTGCACGaa                            <  4:4750/136‑1 (MQ=255)                                                                                                cAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGaa                            <  3:345182/136‑1 (MQ=255)                                                                                                   cTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGAGGCGGCGAGTTAGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACg                         <  6:8505/136‑1 (MQ=255)                                                                                                      cccGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACggg                       <  2:173919/135‑1 (MQ=255)                                                                                                      cccGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACggg                       <  2:178775/135‑1 (MQ=255)                                                                                                        cgccacccAACGACCTCATTTCCCGCAGCATCGGCGGCGAGGTTGCAGTTCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGACCAGGCTCTGGAGGCAACGTTGCATAGCCTGCACGAAACGGGGGc                    <  6:91161/130‑1 (MQ=255)                                                                                                        cGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGCGGCAACGTTGCAGAGCCTGCACGAAACGGGGGc                    <  3:340189/136‑1 (MQ=255)                                                                                                              ccAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCTGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGa              <  5:106042/136‑1 (MQ=255)                                                                                                                     ccTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  1:221817/136‑1 (MQ=255)                                                                                                                     ccTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  6:293528/136‑1 (MQ=255)                                                                                                                     ccTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  5:141528/136‑1 (MQ=255)                                                                                                                     ccTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGAc       <  1:124674/136‑1 (MQ=255)                                                                                                                          tttCCCGCCGCCGCGGCGGCGAGTGTGCAGTGCTGGCACCGGGCATGGTGCAGGAGGATGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  8:304299/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGGTGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTTAACGAATATTTTTCCAGTGATATCGACCAGGt  <  6:94531/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  1:88616/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  6:166505/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  4:92346/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  1:162546/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCAATAAACGGGGGCCAGTGATATCGACCCGGt  <  2:29272/136‑1 (MQ=255)                                                                                                                          tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGACGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  2:308981/136‑1 (MQ=255)                                                                                                                          gttCCCGCATCCGCGGCTGGTCGTTTGCAGTGCGGTCCCCGGGCATGGGGCATGAGGAGGCAGTAAACCTTGCCCAGGCTCTGGAGGCAACGTTGCATAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  8:299853/135‑1 (MQ=255)                                                                                                                               |                                                                                                                                   TTCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACA‑GCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  ACB143/179359‑179614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑