Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 2,143,429 C→T E50K (GAA→AAA)  rne ← Ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,143,4290CT98.2% 190.3 / ‑4.2 55E50K (GAA→AAA) rneRibonuclease E
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (26/28);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GCTGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTCGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGTTGAGTCGCGTTAA  >  ACB143/2143298‑2143560
                                                                                                                                   |                                                                                                                                   
gCTGAGCTCTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTTAAGTACTCGCGGGAGTTTTCTTTGAGCGGCTTGTAGGCGTGAGGTTCGGAGCCGAAGTCGACGTAGGCGGCTTCGAGGCTGGGTTTGATg                                                                                                                                 <  3:130339/136‑1 (MQ=255)
gCTGAGCACTTCCTTGTTGTTTCCCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGTGATATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATg                                                                                                                                 <  7:313959/136‑1 (MQ=255)
 cTGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGc                                                                                                                                >  1:296567/1‑136 (MQ=255)
 cTGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGc                                                                                                                                <  1:323791/136‑1 (MQ=255)
 cTGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGc                                                                                                                                >  2:78498/1‑136 (MQ=255)
 cTGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTCGAt                                                                                                                                  >  8:245556/1‑134 (MQ=255)
  tGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGTCGGCTTCGAGGCTGGGTTTGATGCg                                                                                                                               >  7:80058/1‑136 (MQ=255)
           ccTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCt                                                                                                                       <  4:201324/135‑1 (MQ=255)
           ccTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCt                                                                                                                       <  5:280523/135‑1 (MQ=255)
           ccTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCt                                                                                                                       <  4:244111/135‑1 (MQ=255)
              tGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGTGTGATCTTGcc                                                                                                                   >  6:107643/1‑136 (MQ=255)
              tGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGcc                                                                                                                   >  7:279968/1‑136 (MQ=255)
                 tGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGCGGCTGGGGTTGATGCGGGTGAAGTTGCCt                                                                                                                  >  2:350525/1‑134 (MQ=255)
                        ccGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATg                                                                                                         >  4:59013/1‑136 (MQ=255)
                        ccGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATg                                                                                                         >  6:299880/1‑136 (MQ=255)
                        ccGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATg                                                                                                         >  2:211236/1‑136 (MQ=255)
                        ccGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATg                                                                                                         >  6:252347/1‑136 (MQ=255)
                        ccGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATg                                                                                                         >  5:188526/1‑136 (MQ=255)
                         cGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGt                                                                                                        >  3:96658/1‑136 (MQ=255)
                              ttCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGcc                                                                                                   >  8:87104/1‑136 (MQ=255)
                                   gggCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATATTGCCTTTGTAGATGTTTGCCtttt                                                                                               >  7:115754/1‑135 (MQ=255)
                                          cttGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTc                                                                                        <  2:330315/135‑1 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCgg                                                                             <  5:95344/134‑1 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGa                                                                            >  4:164762/1‑135 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           >  6:145021/1‑136 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           <  6:50131/136‑1 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           >  4:250083/1‑136 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           <  4:339493/136‑1 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           >  2:335762/1‑136 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           >  3:167845/1‑136 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           >  3:133310/1‑136 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTGCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           <  4:282142/136‑1 (MQ=255)
                                                      gcgGGAGATTTCTTTCAGCGGCAGGAAGCCGGGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGAc                                                                           <  6:208750/136‑1 (MQ=255)
                                                       cgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACt                                                                          >  2:254379/1‑136 (MQ=255)
                                                       cgGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACt                                                                          >  2:345749/1‑136 (MQ=255)
                                                            taTTTCTTTCAGCGGCAGGAAGCCGTGACGGTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATg                                                                     <  6:55477/135‑1 (MQ=255)
                                                              tttctttcAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCCGCTCGCGCGCGCCGGACTCGATGTc                                                                   >  2:296206/1‑136 (MQ=255)
                                                                                 gCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTgg                                                <  7:227026/136‑1 (MQ=255)
                                                                                 gCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTgg                                                <  6:50612/136‑1 (MQ=255)
                                                                                      gACGTTCGGAGCCGAAGGCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGCTGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTc                                           <  5:239050/136‑1 (MQ=255)
                                                                                       aCGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTcc                                          <  3:11646/136‑1 (MQ=255)
                                                                                       aCGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTc                                           <  1:110764/135‑1 (MQ=255)
                                                                                        cGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTcca                                         <  5:78283/136‑1 (MQ=255)
                                                                                         gTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTccac                                        <  8:14605/136‑1 (MQ=255)
                                                                                                  cGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTcc                                          >  4:211663/1‑125 (MQ=255)
                                                                                                  cGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGAAGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTcc                                          <  3:211663/125‑1 (MQ=255)
                                                                                                    aaGTCGACGAAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTacac                             <  3:247048/136‑1 (MQ=255)
                                                                                                         gacgaAGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAAc                        <  7:100410/136‑1 (MQ=255)
                                                                                                          ctgaAGGGGGGTTGGAGGCTGGGTTTGATGCTGGTGATCTTGCCTTTGTATTTGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAAc                        <  8:115754/133‑1 (MQ=255)
                                                                                                              aGGCGGCTTCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGAGCGCGCCGGACTCGATGTCCAGGTCGTAGACACGTTGGCCGTCAACCAGGGCTACACGCACATCTTc                   >  2:344661/1‑136 (MQ=255)
                                                                                                                   gcTTCGAGGCTGGGTTTGTTGGGGGTGTTCTTGCGTTTGTAGATGTTGGCGTTTTTCTGCTGGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGAGGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGtt              <  3:183817/136‑1 (MQ=255)
                                                                                                                      tCGAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGTTGAg           >  3:78143/1‑136 (MQ=255)
                                                                                                                        gAGGCTGGGTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGTCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGTTGAGTc         <  5:257516/136‑1 (MQ=255)
                                                                                                                               ggTTTGATGCTGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCTCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGTTGAGTCGCGTTaa  <  1:147431/136‑1 (MQ=255)
                                                                                                                               ggTTTGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGTTGAGTCGCGtt    <  2:23320/134‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
GCTGAGCACTTCCTTGATGTTGACCCGGCCTTCCGGGGCCTTCTTGAAGTACTCGCGGGAGATTTCTTTCAGCGGCAGGAAGCCGTGACGTTCGGAGCCGAAGTCGACGAAGGCGGCTTCGAGGCTGGGTTCGATGCGGGTGATCTTGCCTTTGTAGATGTTGGCCTTTTTCTGCTCGCGCGCGCCGGACTCGATGTCCAGGTCGTAGAGACGTTGGCCGTCCACCAGGGCTACACGCAACTCTTCGGGTTGAGTCGCGTTAA  >  ACB143/2143298‑2143560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑